Cloned (Comment) | Organism |
---|---|
gene PMT1231, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain BW25113(DE3) DELTA13 | Prochlorococcus marinus |
Protein Variants | Comment | Organism |
---|---|---|
I127G | site-directed mutagenesis, increased activity compared to wild-type | Prochlorococcus marinus |
I127G/A48G | site-directed mutagenesis, increased activity compared to wild-type | Prochlorococcus marinus |
additional information | screening for Prochlorococcus marinus enzyme ADO mutants generated by engineering the active center to accommodate branched-chain isobutyraldehyde, identification of two ADO mutants, I127G and I127G/A48G, which exhibit higher catalytic activity for isobutyraldehyde and 3fold improved propane productivity compared to wild-type, propane biosynthesis generation | Prochlorococcus marinus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a long-chain aldehyde + O2 + 2 NADPH + 2 H+ | Prochlorococcus marinus | - |
an alkane + formate + H2O + 2 NADP+ | - |
? | |
a long-chain aldehyde + O2 + 2 NADPH + 2 H+ | Prochlorococcus marinus MIT 9313 | - |
an alkane + formate + H2O + 2 NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Prochlorococcus marinus | Q7V6D4 | - |
- |
Prochlorococcus marinus MIT 9313 | Q7V6D4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a long-chain aldehyde + O2 + 2 NADPH + 2 H+ | - |
Prochlorococcus marinus | an alkane + formate + H2O + 2 NADP+ | - |
? | |
a long-chain aldehyde + O2 + 2 NADPH + 2 H+ | - |
Prochlorococcus marinus MIT 9313 | an alkane + formate + H2O + 2 NADP+ | - |
? | |
isobutyraldehyde + O2 + 2 NADPH + 2 H+ | low activity with the wild-type enzyme, but increased activity with enzyme mutants I127G and I127G/A48G | Prochlorococcus marinus | propane + formate + H2O + 2 NADP+ | - |
? | |
isobutyraldehyde + O2 + 2 NADPH + 2 H+ | low activity with the wild-type enzyme, but increased activity with enzyme mutants I127G and I127G/A48G | Prochlorococcus marinus MIT 9313 | propane + formate + H2O + 2 NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADO | - |
Prochlorococcus marinus |
aldehyde-deformylating oxygenase | - |
Prochlorococcus marinus |
PMT1231 | - |
Prochlorococcus marinus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Prochlorococcus marinus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Prochlorococcus marinus |
General Information | Comment | Organism |
---|---|---|
physiological function | enzyme ADO natively catalyzes the conversion of long-chain aldehydes into corresponding alkanes. To convert short-chain isobutyraldehyde into propane efficiently, the substrate specificity of ADO has to be modified for the utilization of the short-chain aldehydes | Prochlorococcus marinus |