BRENDA - Enzyme Database show
show all sequences of 4.1.99.5

Solubilization, partial purification, and characterization of a fatty aldehyde decarbonylase from a higher plant, Pisum sativum

Schneider-Belhaddad, F.; Kolattukudy, P.; Arch. Biochem. Biophys. 377, 341-349 (2000)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
phopshatidylserine
requirement, phosphatidylcholine is preferred, phopshatidylserine and phopshatidylethanolamine substitute less effectively
Pisum sativum
phosphatidylcholine
requirement, preferred lipid, phopshatidylserine and phopshatidylethanolamine substitute less effectively
Pisum sativum
phosphatidylethanolamine
requirement, phosphatidylcholine is preferred, phopshatidylserine and phopshatidylethanolamine substitute less effectively
Pisum sativum
Phospholipid
requirement, dose-dependent stimulation, phosphatidylcholine is preferred, phopshatidylserine and phopshatidylethanolamine substitute less effectively
Pisum sativum
Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
1 mM, complete inhibition, CuCl2 restores, CoCl2 and ZnCl2 partially restore activity; strong
Pisum sativum
dithioerythritol
5 mM, complete inhibition
Pisum sativum
EDTA
10 mM, 58% inhibition
Pisum sativum
metal ion chelators
Cu2+ fully restores activity; strong
Pisum sativum
NADH
presence causes 50% inhibition
Pisum sativum
NADPH
presence causes 50% inhibition
Pisum sativum
O2
complete inhibition
Pisum sativum
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
membrane-associated
Pisum sativum
16020
-
microsome
membrane-associated
Pisum sativum
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Cu2+
probably a copper enzyme
Pisum sativum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
67000
-
x * 67000, SDS-PAGE
Pisum sativum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Pisum sativum
alkane biosynthesis
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pisum sativum
-
L. V Early Frosty
-
Oxidation Stability
Oxidation Stability
Organism
inhibited in the presence of O2
Pisum sativum
Purification (Commentary)
Commentary
Organism
partial, 1306fold, solubilized with beta-octyl glucoside
Pisum sativum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
young rapidly growing
Pisum sativum
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.000235
-
at room temperature
Pisum sativum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
alkane biosynthesis
648395
Pisum sativum
?
-
-
-
-
octadecanal
-
648395
Pisum sativum
heptadecane + CO
-
648395
Pisum sativum
-
Subunits
Subunits
Commentary
Organism
?
x * 67000, SDS-PAGE
Pisum sativum
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
additional information
-
assay at room temperature
Pisum sativum
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
phopshatidylserine
requirement, phosphatidylcholine is preferred, phopshatidylserine and phopshatidylethanolamine substitute less effectively
Pisum sativum
phosphatidylcholine
requirement, preferred lipid, phopshatidylserine and phopshatidylethanolamine substitute less effectively
Pisum sativum
phosphatidylethanolamine
requirement, phosphatidylcholine is preferred, phopshatidylserine and phopshatidylethanolamine substitute less effectively
Pisum sativum
Phospholipid
requirement, dose-dependent stimulation, phosphatidylcholine is preferred, phopshatidylserine and phopshatidylethanolamine substitute less effectively
Pisum sativum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
1 mM, complete inhibition, CuCl2 restores, CoCl2 and ZnCl2 partially restore activity; strong
Pisum sativum
dithioerythritol
5 mM, complete inhibition
Pisum sativum
EDTA
10 mM, 58% inhibition
Pisum sativum
metal ion chelators
Cu2+ fully restores activity; strong
Pisum sativum
NADH
presence causes 50% inhibition
Pisum sativum
NADPH
presence causes 50% inhibition
Pisum sativum
O2
complete inhibition
Pisum sativum
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
membrane-associated
Pisum sativum
16020
-
microsome
membrane-associated
Pisum sativum
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Cu2+
probably a copper enzyme
Pisum sativum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
67000
-
x * 67000, SDS-PAGE
Pisum sativum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Pisum sativum
alkane biosynthesis
?
-
-
-
Oxidation Stability (protein specific)
Oxidation Stability
Organism
inhibited in the presence of O2
Pisum sativum
Purification (Commentary) (protein specific)
Commentary
Organism
partial, 1306fold, solubilized with beta-octyl glucoside
Pisum sativum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
young rapidly growing
Pisum sativum
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.000235
-
at room temperature
Pisum sativum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
alkane biosynthesis
648395
Pisum sativum
?
-
-
-
-
octadecanal
-
648395
Pisum sativum
heptadecane + CO
-
648395
Pisum sativum
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 67000, SDS-PAGE
Pisum sativum
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
additional information
-
assay at room temperature
Pisum sativum
Other publictions for EC 4.1.99.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747409
Wang
Identification of residues im ...
Synechococcus elongatus PCC 7942, Synechococcus elongatus PCC 7942 R2, Synechocystis sp. PCC 6803
BMC Biotechnol.
17
31-39
2017
-
-
-
-
21
-
-
8
-
2
-
3
-
9
-
-
-
-
-
-
-
-
11
1
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-
-
28
-
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1
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1
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21
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8
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2
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3
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11
1
-
-
-
28
-
-
-
-
-
2
2
-
9
9
748564
Patrikainen
Comparison of orthologous cya ...
Nostoc punctiforme, Nostoc punctiforme ATCC 29133 / PCC 73102, Prochlorococcus marinus, Prochlorococcus marinus MIT 9313, Synechococcus sp. RS9917, Synechocystis sp. PCC 6803
Metab. Eng. Commun.
5
9-18
2017
-
-
4
-
5
-
-
-
-
-
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6
-
13
-
-
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30
-
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-
-
-
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4
-
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4
4
-
5
-
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-
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6
-
-
-
-
-
-
-
-
30
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
746976
Park
Crystal structures of aldehyd ...
Limnothrix redekei, Limnothrix redekei KNUA012, Oscillatoria sp. KNUA011
Biochem. Biophys. Res. Commun.
477
395-400
2016
-
2
2
2
-
-
-
-
-
-
-
3
-
5
-
-
-
-
-
-
-
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6
2
2
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2
-
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2
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2
2
2
2
-
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-
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3
-
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-
-
-
-
-
6
2
2
-
-
-
2
-
-
-
-
4
4
-
-
-
747351
Bao
Structure-oriented substrate ...
Synechococcus elongatus PCC 7942, Synechococcus elongatus PCC 7942 R2
Biotechnol. Biofuels
9
185
2016
-
-
1
-
13
-
-
7
-
-
-
2
-
6
-
-
1
-
-
-
-
-
14
-
1
-
-
8
1
-
-
1
-
-
-
-
-
1
1
-
13
-
-
-
-
7
-
-
-
2
-
-
-
1
-
-
-
-
14
-
1
-
-
8
1
-
-
-
-
2
2
-
8
8
747353
Zhang
Microbial synthesis of propan ...
Prochlorococcus marinus, Prochlorococcus marinus MIT 9313
Biotechnol. Biofuels
9
80
2016
-
-
1
-
3
-
-
-
-
-
-
2
-
7
-
-
-
-
-
-
-
-
4
-
1
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1
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1
1
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3
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2
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-
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4
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
747081
Warui
Efficient delivery of long-ch ...
Nostoc punctiforme, Nostoc punctiforme ATCC 29133 / PCC 73102
Biochemistry
54
1006-1015
2015
-
-
-
-
-
-
-
-
-
-
-
2
-
9
-
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2
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1
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1
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2
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-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
748027
Rajakovich
Rapid reduction of the diferr ...
Nostoc punctiforme, Nostoc punctiforme ATCC 29133 / PCC 73102
J. Am. Chem. Soc.
137
11695-11709
2015
-
-
-
-
-
-
-
-
-
1
-
2
-
8
-
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1
-
-
-
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10
-
1
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-
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1
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2
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2
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1
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2
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-
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-
-
10
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
748032
Shokri
Conversion of aldehyde to alk ...
Prochlorococcus marinus, Prochlorococcus marinus MIT 9313
J. Am. Chem. Soc.
137
7686-7691
2015
-
1
-
-
-
-
-
-
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1
-
2
-
6
-
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1
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8
1
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1
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1
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1
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1
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2
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8
1
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-
-
-
2
2
-
-
-
749051
Hayashi
Role of cysteine residues in ...
Nostoc punctiforme, Nostoc punctiforme ATCC 29133 / PCC 73102
PLoS ONE
10
e0122217
2015
-
-
1
-
8
-
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1
-
1
-
2
-
7
-
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1
-
-
-
-
-
10
-
1
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5
-
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1
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1
1
-
8
-
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1
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1
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2
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1
-
-
-
-
10
-
1
-
5
-
-
-
-
-
-
3
3
-
-
-
749212
Jia
Structural insights into the ...
Synechococcus elongatus PCC 7942, Synechococcus elongatus PCC 7942 R2
Protein Cell
6
55-67
2015
-
-
1
1
3
-
-
-
-
1
-
2
-
6
-
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1
1
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6
1
1
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1
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1
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1
1
1
3
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1
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2
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1
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6
1
1
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-
1
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4
4
-
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-
726552
Das
Mechanistic insights from reac ...
Nostoc punctiforme
ACS Chem. Biol.
9
570-577
2014
-
-
1
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1
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1
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1
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2
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1
1
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6
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1
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3
1
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2
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1
2
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1
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1
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1
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1
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6
-
1
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3
1
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1
1
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746597
Buer
Insights into substrate and m ...
Prochlorococcus marinus, Prochlorococcus marinus MIT 9313
ACS Chem. Biol.
9
2584-2593
2014
-
-
-
1
1
-
1
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1
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2
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6
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1
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8
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1
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1
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1
1
1
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1
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1
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2
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8
-
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1
-
-
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-
-
2
2
-
-
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747074
Waugh
Solvent isotope effects on al ...
Prochlorococcus marinus, Prochlorococcus marinus MIT9313
Biochemistry
53
5537-5543
2014
-
-
1
-
-
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-
1
-
1
-
2
1
3
-
-
1
1
-
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6
-
1
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1
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1
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1
1
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1
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1
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2
1
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1
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6
-
1
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1
-
-
-
-
1
1
-
-
-
726548
Aukema
Cyanobacterial aldehyde deform ...
Prochlorococcus marinus, Prochlorococcus marinus MIT 9313
ACS Catal.
3
2228-2238
2013
-
-
1
-
-
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-
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6
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1
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8
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1
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1
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1
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1
1
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1
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8
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1
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1
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727215
Zhang
Conversion of fatty aldehydes ...
Synechococcus elongatus
Biotechnol. Biofuels
6
86
2013
-
-
1
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1
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1
1
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3
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1
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5
1
1
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1
1
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3
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1
3
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1
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1
1
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1
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5
1
1
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1
1
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727312
Khara
Production of propane and othe ...
Prochlorococcus marinus, Prochlorococcus marinus MIT9313
ChemBioChem
14
1204-1208
2013
-
-
1
-
4
-
-
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1
-
2
-
3
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1
1
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8
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1
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1
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1
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1
1
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4
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1
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2
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1
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8
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1
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1
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1
1
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727690
Pandelia
Substrate-triggered addition o ...
Nostoc punctiforme
J. Am. Chem. Soc.
135
15801-15812
2013
1
-
1
-
-
-
-
1
-
1
-
1
-
2
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1
1
-
-
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2
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1
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1
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2
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1
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1
1
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727693
Paul
Probing the mechanism of cyano ...
Nostoc punctiforme
J. Am. Chem. Soc.
135
5234-5237
2013
-
-
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1
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1
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1
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3
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1
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1
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727001
Li
Evidence for only oxygenative ...
Prochlorococcus marinus
Biochemistry
51
7908-7916
2012
-
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1
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-
-
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4
-
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3
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3
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1
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4
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726957
Eser
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4
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4
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15
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4
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4
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713511
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4
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7
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1
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1
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2
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1
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Cheesbrough
Alkane biosynthesis by decarbo ...
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