BRENDA - Enzyme Database
show all sequences of 4.1.99.5

Alkane biosynthesis by decarbonylation of aldehyde catalyzed by a microsomal preparation from Botryococcus braunii

Dennis, M.W.; Kolattukubdy, P.E.; Arch. Biochem. Biophys. 287, 268-275 (1991)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
ascorbate
2 mM, stimulates nearly 3fold
Botryococcus braunii
Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
strong
Botryococcus braunii
8-hydroxyquinoline
strong
Botryococcus braunii
EDTA
strong
Botryococcus braunii
metal ion chelators
strong
Botryococcus braunii
O2
-
Botryococcus braunii
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.065
-
octadecanal
25C
Botryococcus braunii
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
membrane-associated
Botryococcus braunii
16020
-
microsome
membrane-associated
Botryococcus braunii
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
probably a metalloenzyme
Botryococcus braunii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Botryococcus braunii
final step in alkane biosynthesis
?
-
-
-
additional information
Botryococcus braunii Austin
final step in alkane biosynthesis
?
-
-
-
Organism
Organism
UniProt
Commentary
Textmining
Botryococcus braunii
-
belonging to the A race
-
Botryococcus braunii Austin
-
belonging to the A race
-
Oxidation Stability
Oxidation Stability
Organism
inhibited in the presence of O2
Botryococcus braunii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
additional information
final step in alkane biosynthesis
648393
Botryococcus braunii
?
-
-
-
-
additional information
final step in alkane biosynthesis
648393
Botryococcus braunii Austin
?
-
-
-
-
octadecanal
-
648393
Botryococcus braunii
heptadecane + CO
-
648393
Botryococcus braunii
-
octadecanal
-
648393
Botryococcus braunii Austin
heptadecane + CO
-
648393
Botryococcus braunii Austin
-
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
-
Botryococcus braunii
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
100
-
boiling destroys enzyme activity
Botryococcus braunii
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
very sharp
Botryococcus braunii
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
8
pH 6: about 10% of activity maximum, pH 8: about 30% of activity maximum
Botryococcus braunii
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no cofactor requirement
Botryococcus braunii
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
ascorbate
2 mM, stimulates nearly 3fold
Botryococcus braunii
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no cofactor requirement
Botryococcus braunii
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
strong
Botryococcus braunii
8-hydroxyquinoline
strong
Botryococcus braunii
EDTA
strong
Botryococcus braunii
metal ion chelators
strong
Botryococcus braunii
O2
-
Botryococcus braunii
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.065
-
octadecanal
25C
Botryococcus braunii
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
membrane-associated
Botryococcus braunii
16020
-
microsome
membrane-associated
Botryococcus braunii
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
probably a metalloenzyme
Botryococcus braunii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Botryococcus braunii
final step in alkane biosynthesis
?
-
-
-
additional information
Botryococcus braunii Austin
final step in alkane biosynthesis
?
-
-
-
Oxidation Stability (protein specific)
Oxidation Stability
Organism
inhibited in the presence of O2
Botryococcus braunii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
additional information
final step in alkane biosynthesis
648393
Botryococcus braunii
?
-
-
-
-
additional information
final step in alkane biosynthesis
648393
Botryococcus braunii Austin
?
-
-
-
-
octadecanal
-
648393
Botryococcus braunii
heptadecane + CO
-
648393
Botryococcus braunii
-
octadecanal
-
648393
Botryococcus braunii Austin
heptadecane + CO
-
648393
Botryococcus braunii Austin
-
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
-
Botryococcus braunii
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
100
-
boiling destroys enzyme activity
Botryococcus braunii
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
very sharp
Botryococcus braunii
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
8
pH 6: about 10% of activity maximum, pH 8: about 30% of activity maximum
Botryococcus braunii
Other publictions for EC 4.1.99.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747409
Wang
Identification of residues im ...
Synechococcus elongatus PCC 7942 = FACHB-805, Synechococcus elongatus PCC 7942 = FACHB-805 R2, Synechocystis sp. PCC 6803
BMC Biotechnol.
17
31-39
2017
-
-
-
-
21
-
-
8
-
2
-
3
-
9
-
-
-
-
-
-
-
-
11
1
7
-
-
-
28
-
-
-
1
-
-
-
-
-
-
1
-
21
-
-
-
-
8
-
2
-
3
-
-
-
-
-
-
-
-
11
1
-
-
-
28
-
-
-
-
-
2
2
-
9
9
748564
Patrikainen
Comparison of orthologous cya ...
Nostoc punctiforme, Nostoc punctiforme ATCC 29133 / PCC 73102, Prochlorococcus marinus, Prochlorococcus marinus MIT 9313, Synechococcus sp. RS9917, Synechocystis sp. PCC 6803
Metab. Eng. Commun.
5
9-18
2017
-
-
4
-
5
-
-
-
-
-
-
6
-
13
-
-
-
-
-
-
-
-
30
-
14
-
-
-
-
-
-
-
4
-
-
-
-
-
4
4
-
5
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
30
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
746976
Park
Crystal structures of aldehyd ...
Limnothrix redekei, Limnothrix redekei KNUA012, Oscillatoria sp. KNUA011
Biochem. Biophys. Res. Commun.
477
395-400
2016
-
2
2
2
-
-
-
-
-
-
-
3
-
5
-
-
-
-
-
-
-
-
6
2
4
2
-
-
-
2
-
-
2
-
-
-
-
2
2
2
2
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
6
2
2
-
-
-
2
-
-
-
-
4
4
-
-
-
747351
Bao
Structure-oriented substrate ...
Synechococcus elongatus PCC 7942 = FACHB-805, Synechococcus elongatus PCC 7942 = FACHB-805 R2
Biotechnol. Biofuels
9
185
2016
-
-
1
-
13
-
-
7
-
-
-
2
-
6
-
-
1
-
-
-
-
-
14
-
6
1
-
-
8
1
-
-
1
-
-
-
-
-
1
1
-
13
-
-
-
-
7
-
-
-
2
-
-
-
1
-
-
-
-
14
-
1
-
-
8
1
-
-
-
-
2
2
-
8
8
747353
Zhang
Microbial synthesis of propan ...
Prochlorococcus marinus, Prochlorococcus marinus MIT 9313
Biotechnol. Biofuels
9
80
2016
-
-
1
-
3
-
-
-
-
-
-
2
-
7
-
-
-
-
-
-
-
-
4
-
3
1
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
747081
Warui
Efficient delivery of long-ch ...
Nostoc punctiforme, Nostoc punctiforme ATCC 29133 / PCC 73102
Biochemistry
54
1006-1015
2015
-
-
-
-
-
-
-
-
-
-
-
2
-
9
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
748027
Rajakovich
Rapid reduction of the diferr ...
Nostoc punctiforme, Nostoc punctiforme ATCC 29133 / PCC 73102
J. Am. Chem. Soc.
137
11695-11709
2015
-
-
-
-
-
-
-
-
-
1
-
2
-
8
-
-
-
1
-
-
-
-
10
-
2
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
10
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
748032
Shokri
Conversion of aldehyde to alk ...
Prochlorococcus marinus, Prochlorococcus marinus MIT 9313
J. Am. Chem. Soc.
137
7686-7691
2015
-
1
-
-
-
-
-
-
-
1
-
2
-
6
-
-
-
1
-
-
-
-
8
1
3
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
8
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
749051
Hayashi
Role of cysteine residues in ...
Nostoc punctiforme, Nostoc punctiforme ATCC 29133 / PCC 73102
PLoS ONE
10
e0122217
2015
-
-
1
-
8
-
-
1
-
1
-
2
-
7
-
-
1
-
-
-
-
-
10
-
2
1
-
5
-
-
-
-
1
-
-
-
-
-
1
1
-
8
-
-
-
-
1
-
1
-
2
-
-
-
1
-
-
-
-
10
-
1
-
5
-
-
-
-
-
-
3
3
-
-
-
749212
Jia
Structural insights into the ...
Synechococcus elongatus PCC 7942 = FACHB-805, Synechococcus elongatus PCC 7942 = FACHB-805 R2
Protein Cell
6
55-67
2015
-
-
1
1
3
-
-
-
-
1
-
2
-
6
-
-
1
1
-
-
-
-
6
1
5
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
1
3
-
-
-
-
-
-
1
-
2
-
-
-
1
-
-
-
-
6
1
1
-
-
-
1
-
-
-
-
4
4
-
-
-
726552
Das
Mechanistic insights from reac ...
Nostoc punctiforme
ACS Chem. Biol.
9
570-577
2014
-
-
1
-
-
-
1
-
-
1
-
1
-
2
-
-
1
1
-
-
-
-
6
-
3
1
-
-
3
1
-
-
2
-
-
-
-
-
1
2
-
-
-
-
1
-
-
-
1
-
1
-
-
-
1
-
-
-
-
6
-
1
-
-
3
1
-
-
-
-
1
1
-
-
-
746597
Buer
Insights into substrate and m ...
Prochlorococcus marinus, Prochlorococcus marinus MIT 9313
ACS Chem. Biol.
9
2584-2593
2014
-
-
-
1
1
-
1
-
-
1
-
2
-
6
-
-
-
1
-
-
-
-
8
-
4
-
-
-
1
-
-
-
1
-
-
-
-
-
-
1
1
1
-
-
1
-
-
-
1
-
2
-
-
-
-
-
-
-
-
8
-
-
-
-
1
-
-
-
-
-
2
2
-
-
-
747074
Waugh
Solvent isotope effects on al ...
Prochlorococcus marinus, Prochlorococcus marinus MIT9313
Biochemistry
53
5537-5543
2014
-
-
1
-
-
-
-
1
-
1
-
2
1
3
-
-
1
1
-
-
-
-
6
-
2
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
1
-
1
-
2
1
-
-
1
-
-
-
-
6
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
726548
Aukema
Cyanobacterial aldehyde deform ...
Prochlorococcus marinus, Prochlorococcus marinus MIT 9313
ACS Catal.
3
2228-2238
2013
-
-
1
-
-
-
-
-
-
-
-
-
-
6
-
-
1
-
-
-
-
-
8
-
3
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
8
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
727215
Zhang
Conversion of fatty aldehydes ...
Synechococcus elongatus, Synechococcus elongatus PCC 7942
Biotechnol. Biofuels
6
86
2013
-
-
1
-
-
-
-
1
-
-
1
2
-
4
-
-
1
-
-
-
-
-
10
1
3
1
-
-
1
1
-
-
3
-
-
-
-
-
1
3
-
-
-
-
-
-
1
-
-
1
2
-
-
-
1
-
-
-
-
10
1
1
-
-
1
1
-
-
-
-
-
-
-
-
-
727312
Khara
Production of propane and othe ...
Prochlorococcus marinus, Prochlorococcus marinus MIT9313
ChemBioChem
14
1204-1208
2013
-
-
1
-
4
-
-
-
-
1
-
2
-
3
-
-
1
1
-
-
-
-
8
-
3
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
4
-
-
-
-
-
-
1
-
2
-
-
-
1
-
-
-
-
8
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
727690
Pandelia
Substrate-triggered addition o ...
Nostoc punctiforme
J. Am. Chem. Soc.
135
15801-15812
2013
1
-
1
-
-
-
-
1
-
1
-
1
-
2
-
-
1
1
-
-
-
-
2
-
2
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
727693
Paul
Probing the mechanism of cyano ...
Nostoc punctiforme
J. Am. Chem. Soc.
135
5234-5237
2013
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
2
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
727001
Li
Evidence for only oxygenative ...
Prochlorococcus marinus
Biochemistry
51
7908-7916
2012
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
3
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
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3
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1
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1
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1
1
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715293
Warui
Detection of formate, rather t ...
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1
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1
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4
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1
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1
1
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715300
Li
Conversion of fatty aldehydes ...
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1
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1
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1
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726957
Eser
Oxygen-independent alkane form ...
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15
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8
4
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4
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4
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4
4
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4
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15
-
4
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4
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4
4
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713511
Schirmer
Microbial biosynthesis of alka ...
Nostoc punctiforme
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1
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1
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1
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1
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1
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1
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1
1
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Schneider-Belhaddad
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4
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2
1
1
1
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4
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1
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1
1
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2
1
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1
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4
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7
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2
1
1
1
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1
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1
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1
1
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2
1
1
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648396
Kobayashi
Cobalt proteins ...
Botryococcus braunii
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1-9
1999
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1
2
1
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1
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1
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648394
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A cobalt-porphyrin enzyme conv ...
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1992
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1
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1
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2
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1
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1
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4
1
1
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1
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4
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1
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1
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1
1
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1
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5
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1
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1
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4
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1
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1
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1
1
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Cheesbrough
Microsomal preparation from an ...
Podiceps nigricollis
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2
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1
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1
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1
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1
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9
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1
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1
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1
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2
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1
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1
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1
1
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648391
Cheesbrough
Alkane biosynthesis by decarbo ...
Pisum sativum
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1984
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-
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2
4
1
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1
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1
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3
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1
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1
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1
1
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1
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1
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