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Literature summary for 4.1.99.5 extracted from
- Alkane biosynthesis by decarbonylation of aldehyde catalyzed by a microsomal preparation from Botryococcus braunii (1991), Arch. Biochem. Biophys., 287, 268-275.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ascorbate | 2 mM, stimulates nearly 3fold | Botryococcus braunii |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | strong | Botryococcus braunii | |
| 8-hydroxyquinoline | strong | Botryococcus braunii | |
| EDTA | strong | Botryococcus braunii | |
| metal ion chelators | strong | Botryococcus braunii | |
| O2 | - |
Botryococcus braunii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.065 | - |
octadecanal | 25°C | Botryococcus braunii | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | membrane-associated | Botryococcus braunii | 16020 | - |
| microsome | membrane-associated | Botryococcus braunii | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | probably a metalloenzyme | Botryococcus braunii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Botryococcus braunii | final step in alkane biosynthesis | ? | - |
? | |
| additional information | Botryococcus braunii Austin | final step in alkane biosynthesis | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Botryococcus braunii | - |
belonging to the A race | - |
| Botryococcus braunii Austin | - |
belonging to the A race | - |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| inhibited in the presence of O2 | Botryococcus braunii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | final step in alkane biosynthesis | Botryococcus braunii | ? | - |
? | |
| additional information | final step in alkane biosynthesis | Botryococcus braunii Austin | ? | - |
? | |
| octadecanal | - |
Botryococcus braunii | heptadecane + CO | - |
? | |
| octadecanal | - |
Botryococcus braunii Austin | heptadecane + CO | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Botryococcus braunii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
boiling destroys enzyme activity | Botryococcus braunii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
very sharp | Botryococcus braunii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | pH 6: about 10% of activity maximum, pH 8: about 30% of activity maximum | Botryococcus braunii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no cofactor requirement | Botryococcus braunii |
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Release 2023.1 (January 2023)
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