Cloned (Comment) | Organism |
---|---|
gene VPA1471 or phr, DNA and amino acid sequence determination and analysis, quantitative real-time PCR expression analysis, functional recombinant expression of His6-tagged enzyme in Escherichia coli from pET-30a vector | Vibrio parahaemolyticus RIMD 2210633 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclobutadipyrimidine (in DNA) | Vibrio parahaemolyticus RIMD 2210633 | - |
2 pyrimidine residues (in DNA) | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio parahaemolyticus RIMD 2210633 | Q87G46 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli | Vibrio parahaemolyticus RIMD 2210633 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclobutadipyrimidine (in DNA) | - |
Vibrio parahaemolyticus RIMD 2210633 | 2 pyrimidine residues (in DNA) | - |
? | |
additional information | usage of salmon sperm DNA with introduced CPDs (UVC-irradiation) as assay substrate | Vibrio parahaemolyticus RIMD 2210633 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 57000, recombinant His6-tagged enzyme, SDS-PAGE | Vibrio parahaemolyticus RIMD 2210633 |
Synonyms | Comment | Organism |
---|---|---|
CPD photolyase | - |
Vibrio parahaemolyticus RIMD 2210633 |
deoxyribodipyrimidine photolyase | - |
Vibrio parahaemolyticus RIMD 2210633 |
PHR | - |
Vibrio parahaemolyticus RIMD 2210633 |
VPA1471 | - |
Vibrio parahaemolyticus RIMD 2210633 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Vibrio parahaemolyticus RIMD 2210633 |
Organism | Comment | Expression |
---|---|---|
Vibrio parahaemolyticus RIMD 2210633 | the VPA1471 gene expression level increases during UVA illumination following UVC irradiation | up |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the cryptochrome/photolyase family (CPF) | Vibrio parahaemolyticus RIMD 2210633 |
physiological function | UV-induced lesions can be repaired by enzymes called photolyases. DNA photolyases use blue/near-UV light to remove these lesions using a process referred to as photoreactivation. CPD photolyases specifically repair CPD lesions in DNA | Vibrio parahaemolyticus RIMD 2210633 |