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Literature summary for 4.1.99.3 extracted from

  • Jiang, T.; Wang, Y.; Tang, Z.; Huang, G.; Xu, H.; Qiao, D.; Cao, Y.
    Repair activity of Gln336 on the FAD binding domain of CPD photolyase in Dunaliella salina under stress (2017), Chin. J. Appl. Environ. Biol., 23, 238-243 .
No PubMed abstract available
Search for more literature for 4.1.99.3

Protein Variants

Protein Variants Comment Organism
additional information comparison of repair activity of the photolyase in the wild-type strain PGEX-4T-1-DsPHR2 and the mutant strain PGEX-4T-1-DsPHR2-Q336H in vitro and in vitro and under different salt concentrations, overview. The mutant shows reduced repair activity compared to wild-type, and the survival rate declines rapidly as salinity increased in the mutant Q336H, while in the wild-type strain, there is no change in the survival rate Dunaliella salina
Q336H site-directed mutagenesis of the alpha13 conserved amino acid residue Dunaliella salina

Inhibitors

Inhibitors Comment Organism Structure
glycerol the influence of glycerol concentration on the enzymatic activity in the Q336H mutant is significantly greater than that in wild-type CPD photolyase (P < 0.05). The repair activity of the mutant enzyme Q336H decreases gradually as glycerol concentration increases, while for wild-type CPD photolyase, the trend for repair activity under different glycerol concentrations first increases and then decreases Dunaliella salina

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cyclobutadipyrimidine (in DNA) Dunaliella salina
-
 2 pyrimidine residues (in DNA)
-
 ?

Organism

Organism UniProt Comment Textmining
Dunaliella salina A0T3C8 i.e. Protococcus salinus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyclobutadipyrimidine (in DNA)
-
 Dunaliella salina 2 pyrimidine residues (in DNA)
-
 ?
additional information comparison of repair activity of the photolyase in the wild-type strain PGEX-4T-1-DsPHR2 and the mutant strain PGEX-4T-1-DsPHR2-Q336H in vitro and in vitro and under different salt concentrations, overview. The mutant shows reduced repair activity compared to wild-type, and the survival rate declines rapidly as salinity increased in the mutant Q336H, while in the wild-type strain, there is no change in the survival rate Dunaliella salina ?
-
 ?

Subunits

Subunits Comment Organism
More secondary structure domain of CPD photolyase prediction Dunaliella salina

Synonyms

Synonyms Comment Organism
CPD photolyase
-
 Dunaliella salina
cyclobutane pyrimidine dimers photolyase
-
 Dunaliella salina
PHR2
-
 Dunaliella salina

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Dunaliella salina

General Information

General Information Comment Organism
physiological function residue Gln336 is important for the repair activity of CPD photolyase in Dunaliella salina and may represent key amino acid residues under salt stress Dunaliella salina