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Literature summary for 4.1.99.3 extracted from

  • Liu, Z.; Tan, C.; Guo, X.; Kao, Y.T.; Li, J.; Wang, L.; Sancar, A.; Zhong, D.
    Dynamics and mechanism of cyclobutane pyrimidine dimer repair by DNA photolyase (2011), Proc. Natl. Acad. Sci. USA, 108, 14831-14836.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.99.3

Protein Variants

Protein Variants Comment Organism
E274A site-directed mutagenesis of a residue near the substrate side Escherichia coli
E274A site-directed mutagenesis of an active site residue Escherichia coli
M345A site-directed mutagenesis of an active site residue Escherichia coli
N378C site-directed mutagenesis of a residue near the flavin cofactor side Escherichia coli
N378C site-directed mutagenesis of an active site residue Escherichia coli
R226A site-directed mutagenesis of an active site residue Escherichia coli
R342A site-directed mutagenesis of an active site residue Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cyclobutadipyrimidine (in DNA) Escherichia coli
-
 2 pyrimidine residues (in DNA)
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Reaction

Reaction Comment Organism Reaction ID
cyclobutadipyrimidine (in DNA) = 2 pyrimidine residues (in DNA) cyclic electron-transfer radical mechanism with two fundamental processes, electron-tunneling pathways and cyclobutane ring splitting, the cyclobutane pyrimidine dimer splits in two sequential steps within 90 ps and the electron tunnels between the cofactor and substrate through a remarkable route with an intervening adenine, dynamics and mechanism of cyclobutane pyrimidine dimer repair by DNA photolyase, overview Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyclobutadipyrimidine (in DNA)
-
 Escherichia coli 2 pyrimidine residues (in DNA)
-
 ?
cyclobutadipyrimidine (in DNA) various CPD substrates, T-T, T-U, U-T, U-U dimers Escherichia coli 2 pyrimidine residues (in DNA)
-
 ?

Synonyms

Synonyms Comment Organism
CPD photolyase
-
 Escherichia coli
DNA photolyase
-
 Escherichia coli
thymine dimer by photolyase
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
FAD absorption spectra of FADH+, FADH radical, and FADH- of wild-type and mutant enzymes, overview. All three flavin species and decays to zero upon completion of repair Escherichia coli

General Information

General Information Comment Organism
additional information electron-tunneling pathways and functional role of adenine moiety of wild-type and mutant enzymes, overview Escherichia coli