Protein Variants | Comment | Organism |
---|---|---|
N341A | life-time of 2.9 ns compared to 1.3 ns for the wild-type enzyme | Escherichia coli |
W395R | fully binds methenyltetrahydrofolate but not fully reduced FADH-, life-time of 2.9 ns | Caulobacter vibrioides |
General Stability | Organism |
---|---|
life-time of wild-type enzyme is 1.3 ns | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caulobacter vibrioides | - |
- |
- |
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Caulobacter vibrioides |
purified with neutral radical FADH and/or oxidized FAD forms and then converted to a fully reduced FADH- form under anaerobic conditions | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclobutadipyrimidine in DNA | - |
Caulobacter vibrioides | ? | - |
? | |
cyclobutadipyrimidine in DNA | presence of a very rigid antenna binding site, a relatively rigid active site in CPD photolyase but with large local orientation flexibility | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CPD photolyase | - |
Escherichia coli |
CPD photolyase | - |
Caulobacter vibrioides |
PHR | - |
Escherichia coli |
PHR | - |
Caulobacter vibrioides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FADH2 | - |
Caulobacter vibrioides | |
FADH2 | heterogeneous dynamics continuously tune local configurations to optimize photolyase's function through resonance energy transfer from the antenna to the cofactor for energy efficiency and then electron transfer between the cofactor and the substrate for repair of damaged DNA | Escherichia coli | |
methenyltetrahydrofolate | - |
Escherichia coli | |
methenyltetrahydrofolate | - |
Caulobacter vibrioides |