Application | Comment | Organism |
---|---|---|
biotechnology | a new class of photolyases with specificity for cyclobutane pyrimidine dimers in ssDNA is defined. Members of these branch are found in bacteria, plants, and animals, and are designated Cry-DASH, because of the lack of significant photorepair activity on dsDNA | Arabidopsis thaliana |
biotechnology | a new class of photolyases with specificity for cyclobutane pyrimidine dimers in ssDNA is defined. Members of these branch are found in bacteria, plants, and animals, and are designated Cry-DASH, because of the lack of significant photorepair activity on dsDNA | Xenopus laevis |
biotechnology | a new class of photolyases with specificity for cyclobutane pyrimidine dimers in ssDNA is defined. Members of these branch are found in bacteria, plants, and animals, and are designated Cry-DASH, because of the lack of significant photorepair activity on dsDNA | Vibrio cholerae serotype O1 |
Cloned (Comment) | Organism |
---|---|
AtCry1 belonging to the cryptochrome/photolyase class of enzymes called Cry-DASH proteins is shown to possess no photorepair activity under any condition | Arabidopsis thaliana |
AtCry3 belonging to the cryptochrome/photolyase class of enzymes called Cry-DASH proteins is shown to be photolyases with high degree of specificity for cyclobutane pyrimidine dimers in ssDNA | Arabidopsis thaliana |
BsUvrC belonging to the cryptochrome/photolyase class of enzymes called Cry-DASH proteins is shown to possess no photorepair activity under any condition | Bacillus subtilis |
VcCry1 belonging to the cryptochrome/photolyase class of enzymes called Cry-DASH proteins is shown to be photolyases with high degree of specificity for cyclobutane pyrimidine dimers in ssDNA | Vibrio cholerae serotype O1 |
XlCry-DASH belonging to the cryptochrome/photolyase class of enzymes called Cry-DASH proteins is shown to be photolyases with high degree of specificity for cyclobutane pyrimidine dimers in ssDNA | Xenopus laevis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Bacillus subtilis | - |
- |
- |
Vibrio cholerae serotype O1 | - |
- |
- |
Xenopus laevis | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
AtCry1 shows no photorepair activity under any condition | Arabidopsis thaliana |
additional information | - |
BsUvrC shows no photorepair activity under any condition | Bacillus subtilis |
additional information | - |
it is shown by in vitro and in vivo by photoreactivation assays and electrophoretic mobility shift assays that AtCry3, as a member of the Cry-DASH enzymes, is specific for cyclobutane pyrimidine dimers in ssDNA | Arabidopsis thaliana |
additional information | - |
it is shown by in vitro and in vivo by photoreactivation assays and electrophoretic mobility shift assays that VcCry1, as a member of the Cry-DASH enzymes,is specific for cyclobutane pyrimidine dimers in ssDNA | Vibrio cholerae serotype O1 |
additional information | - |
it is shown by in vitro and in vivo by photoreactivation assays and electrophoretic mobility shift assays that XlCry-DASH, as a member of the Cry-DASH enzymes, is specific for cyclobutane pyrimidine dimers in ssDNA | Xenopus laevis |
additional information | - |
it is shown that VcCry1 repairs photodimers in the deoxyribonucleotide homopolymers poly dU (uracil cyclobutane dimer) and poly dT much more efficiently than the dimers in the ribopolynucleotide homopolymer | Vibrio cholerae serotype O1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclobutadipyrimidine in DNA | AtCry3 repairs the dimer but only in ssDNA | Arabidopsis thaliana | 2 pyrimidine residues in DNA | - |
? | |
cyclobutadipyrimidine in DNA | VcCry1 repairs the dimer but only in ssDNA | Vibrio cholerae serotype O1 | 2 pyrimidine residues in DNA | - |
? | |
cyclobutadipyrimidine in DNA | XlCry-DASH repairs the dimer but only in ssDNA | Xenopus laevis | 2 pyrimidine residues in DNA | - |
? | |
cyclobutadipyrimidine in RNA | - |
Arabidopsis thaliana | 2 pyrimidine residues in RNA | - |
? | |
cyclobutadipyrimidine in RNA | - |
Xenopus laevis | 2 pyrimidine residues in RNA | - |
? | |
cyclobutadipyrimidine in RNA | - |
Vibrio cholerae serotype O1 | 2 pyrimidine residues in RNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AtCry1 | - |
Arabidopsis thaliana |
AtCry3 | - |
Arabidopsis thaliana |
BsUvrC | - |
Bacillus subtilis |
Cry-DASH | - |
Bacillus subtilis |
Cry-DASH | - |
Arabidopsis thaliana |
Cry-DASH | - |
Xenopus laevis |
Cry-DASH | - |
Vibrio cholerae serotype O1 |
VcCry1 | - |
Vibrio cholerae serotype O1 |
XlCry-DASH | - |
Xenopus laevis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Arabidopsis thaliana |
7.5 | - |
assay at | Xenopus laevis |
7.5 | - |
assay at | Vibrio cholerae serotype O1 |