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Literature summary for 4.1.99.24 extracted from
- Three-dimensional structures of Pseudomonas aeruginosa PvcA and PvcB, two proteins involved in the synthesis of 2-isocyano-6,7-dihydroxycoumarin (2008), J. Mol. Biol., 384, 193-205 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21(DE3) | Pseudomonas aeruginosa |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| crystals are grown at 20°C by hanging drop vapor diffusion using a 1:1 mixture of protein and precipitant containing 41-45% PEG 1000, 35-50 mM KH2PO4, and 100 mM Tris (pH 9.0). The structure of PvcA demonstrates an enzyme architecture that is built upon a Rossmann fold | Pseudomonas aeruginosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | Q9I1L5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas aeruginosa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pvcA | - |
Pseudomonas aeruginosa |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | strains harboring a disruption in the pvcA or pvcB genes are able to grow in iron depleted conditions and to produce pyoverdine | Pseudomonas aeruginosa |
| metabolism | the enzyme is involved in the synthesis of 2-isocyano-6,7-dihydroxycoumarin | Pseudomonas aeruginosa |
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