Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Staphylococcus aureus |
Protein Variants | Comment | Organism |
---|---|---|
D198A | mutation in GG motif, decrease in affinity for S-adenosine-L-methionine | Staphylococcus aureus |
G339A | mutation in GG motif, loss of activity | Staphylococcus aureus |
G340A | mutation in GG motif, loss of activity | Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0031 | - |
GTP | wild-type, pH 7.6, 25°C | Staphylococcus aureus | |
0.0051 | - |
S-adenosyl-L-methionine | wild-type, pH 7.6, 25°C | Staphylococcus aureus | |
0.0069 | - |
GTP | mutant D198A, pH 7.6, 25°C | Staphylococcus aureus | |
0.036 | - |
S-adenosyl-L-methionine | mutant D198A, pH 7.6, 25°C | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + S-adenosyl-L-methionine + reduced electron acceptor | Staphylococcus aureus | - |
(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + oxidized electron acceptor | - |
? | |
GTP + S-adenosyl-L-methionine + reduced electron acceptor | Staphylococcus aureus N315 | - |
(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + oxidized electron acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | P65388 | - |
- |
Staphylococcus aureus N315 | P65388 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + S-adenosyl-L-methionine + reduced electron acceptor | - |
Staphylococcus aureus | (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + oxidized electron acceptor | - |
? | |
GTP + S-adenosyl-L-methionine + reduced electron acceptor | - |
Staphylococcus aureus N315 | (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + oxidized electron acceptor | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MoaA | - |
Staphylococcus aureus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00007 | - |
S-adenosyl-L-methionine | mutant D198A, pH 7.6, 25°C | Staphylococcus aureus | |
0.00008 | - |
GTP | mutant D198A, pH 7.6, 25°C | Staphylococcus aureus | |
0.0007 | - |
GTP | wild-type, pH 7.6, 25°C | Staphylococcus aureus | |
0.00075 | - |
S-adenosyl-L-methionine | wild-type, pH 7.6, 25°C | Staphylococcus aureus |
General Information | Comment | Organism |
---|---|---|
physiological function | the GG motif is essential for the activity of MoaA to produce (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate from GTP, and synthetic peptides corresponding to the C-terminal region of wild-type MoaA rescue the GTP 3',8-cyclase activity of the GG-motif mutants. The C-terminal tail containing the GG motif interacts with the SAM-binding pocket of MoaA, and is essential for the binding of SAM and subsequent radical initiation | Staphylococcus aureus |