Literature summary for 4.1.99.22 extracted from

Kanaujia, S.P.; Jeyakanthan, J.; Shinkai, A.; Kuramitsu, S.; Yokoyama, S.; Sekar, K.
Crystal structures, dynamics and functional implications of molybdenum-cofactor biosynthesis protein MogA from two thermophilic organisms (2011), Acta Crystallogr. Sect. F, 67, 2-16.

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Cloned(Commentary)

Cloned (Comment)	Organism
gene mog, phylogenetic tree, expression of His-tagged enzyme in Escherichia coli strain BL21-CodonPlus(DE3)-RIL	Aquifex aeolicus
gene mogA, phylogenetic tree, recombinant expression	Thermus thermophilus

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme, mixing of 0.001 ml of 24 mg/ml protein in 20 mM Tris-HCl, pH 8.0, and 0.2 M NaCl, with 0.001 ml of reservoir solution containing 40% v/v PEG 600, 100 mM CHES buffer, pH 9.5, or 0.2 M ammonium acetate, 0.1 M Bis-Tris, pH 5.5, 25% w/v PEG 3350, 1 week, two different crystal forms, X-ray diffraction structure determination and analysis at 1.7-1.9 A resolution, molecular replacement method	Aquifex aeolicus
purified recombinant enzyme, X-ray diffraction structure determination and analysis at 1.64 A resolution, molecular replacement method	Thermus thermophilus

Crystallization (Comment)

Organism

purified recombinant enzyme, mixing of 0.001 ml of 24 mg/ml protein in 20 mM Tris-HCl, pH 8.0, and 0.2 M NaCl, with 0.001 ml of reservoir solution containing 40% v/v PEG 600, 100 mM CHES buffer, pH 9.5, or 0.2 M ammonium acetate, 0.1 M Bis-Tris, pH 5.5, 25% w/v PEG 3350, 1 week, two different crystal forms, X-ray diffraction structure determination and analysis at 1.7-1.9 A resolution, molecular replacement method

Aquifex aeolicus

purified recombinant enzyme, X-ray diffraction structure determination and analysis at 1.64 A resolution, molecular replacement method

Thermus thermophilus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	molecular dynamics and docking studies with several small molecules, including substrate and product, binding affinities towards AaMogA, overview	Aquifex aeolicus
additional information	-	additional information	molecular dynamics and docking studies with several small molecules, including substrate and product, binding affinities towards TtMogA, overview	Thermus thermophilus

Organism

Organism	UniProt	Comment	Textmining
Aquifex aeolicus	O66472	mog gene, aq_061	-
Thermus thermophilus	Q5SLF2	gene TTHA0341	-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579	Q5SLF2	gene TTHA0341	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme	Thermus thermophilus
recombinant enzyme from Escherichia coli strain BL21-CodonPlus(DE3)-RIL by two different times of gel filtration and anion exchange chromatography, followed by gel filtration and hydroxyapatite chromatography, and a last step of gel filtration	Aquifex aeolicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
additional information	binding energetics of substrates and compounds, e.g. GTP, GMP, GDP, AMP, ADP, ATP, and molydopterin, docking study, overview	Thermus thermophilus	?	-	?
additional information	binding energetics of substrates and compounds, e.g. GTP, GMP, GDP, AMP, ADP, ATP, and molydopterin, docking study, overview	Aquifex aeolicus	?	-	?
additional information	binding energetics of substrates and compounds, e.g. GTP, GMP, GDP, AMP, ADP, ATP, and molydopterin, docking study, overview	Thermus thermophilus HB8 / ATCC 27634 / DSM 579	?	-	?

Subunits

Subunits	Comment	Organism
More	homology structure comparisons for identification of residues involved in protein oligomerization, overview	Thermus thermophilus
More	homology structure comparisons for identification of residues involved in protein oligomerization, overview	Aquifex aeolicus

Synonyms

Synonyms	Comment	Organism
Moco-biosynthesis protein	-	Thermus thermophilus
Moco-biosynthesis protein	-	Aquifex aeolicus
MogA	-	Thermus thermophilus
MogA	-	Aquifex aeolicus
molybdenum-cofactor biosynthesis protein	-	Thermus thermophilus
molybdenum-cofactor biosynthesis protein	-	Aquifex aeolicus