Literature summary for 4.1.99.20 extracted from

Kawaguchi, H.; Sasaki, K.; Uematsu, K.; Tsuge, Y.; Teramura, H.; Okai, N.; Nakamura-Tsuruta, S.; Katsuyama, Y.; Sugai, Y.; Ohnishi, Y.; Hirano, K.; Sazuka, T.; Ogino, C.; Kondo, A.
3-Amino-4-hydroxybenzoic acid production from sweet sorghum juice by recombinant Corynebacterium glutamicum (2015), Bioresour. Technol., 198, 410-417 .

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Application

Application	Comment	Organism
synthesis	sweet sorghum juice is a potentially suitable feedstock for 3,4-AHBA production by recombinant Corynebacterium glutamicum expressing enzyme GriH from Streptomyces griseus. 3-Amino-4-hydroxybenzoic acid (3,4-AHBA) is a precursor for production of the bioplastic	Streptomyces griseus

Cloned(Commentary)

Cloned (Comment)	Organism
gene griH, functional recombinant expression of the enzyme in Corynebacterium glutamicum strain KT01, leucine specifically enhances 3-amino-4-hydroxybenzoate production by strain KT01	Streptomyces griseus

Protein Variants

Protein Variants	Comment	Organism
additional information	recombinant Corynebacterium glutamicum strain KT01 expressing griH and griI derived from Streptomyces griseus produces 3-amino-4-hydroxybenzoate from the sweet sorghum juice of cultivar SIL-05 at a final concentration (1.0 g/l) that is 5fold higher than that from pure sucrose. Fractionation of sweet sorghum juice by nanofiltration (NF) membrane separation (molecular weight cut-off 150) reveals that the NF-concentrated fraction, which contains the highest concentrations of amino acids, increases 3-amino-4-hydroxybenzoate production, whereas the NF-filtrated fraction inhibits 3,4-AHBA biosynthesis. Amino acid supplementation experiments reveal that leucine specifically enhances 3-amino-4-hydroxybenzoate production by strain KT01. Recombinant strain KT01 was aerobically cultured. Method optimization and evaluation, overview	Streptomyces griseus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate	Streptomyces griseus	-	3-amino-4-hydroxybenzoate + phosphate + 2 H2O	-	?
additional information	Streptomyces griseus	3-amino-4-hydroxybenzoic acid production from sweet Sorghum juice by recombinant Corynebacterium glutamicum	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Streptomyces griseus	A0JC76	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate	-	Streptomyces griseus	3-amino-4-hydroxybenzoate + phosphate + 2 H2O	-	?
additional information	3-amino-4-hydroxybenzoic acid production from sweet Sorghum juice by recombinant Corynebacterium glutamicum	Streptomyces griseus	?	-	?

Synonyms

Synonyms	Comment	Organism
griH	-	Streptomyces griseus

Expression

Organism	Comment	Expression
Streptomyces griseus	leucine induces the recombinant enzyme expression in Corynebacterium glutamicum strain KT01	up

General Information

General Information	Comment	Organism
physiological function	3-amino-4-hydroxybenzoic acid (3,4-AHBA) is a metabolic intermediate of grixazone biosynthesis in Streptomyces griseus and is synthesized by the activities of the enzymes GriI and GriH, which are encoded by griI and griH, respectively. GriI catalyzes an aldol condensation reaction between L-aspartate-4-semialdehyde and dihydroxyacetone phosphate, and GriH converts the resulting C7 metabolite into 3,4-AHBA. Through this two-step reaction, these two enzymes can produce an aromatic ring from C4 (L-aspartate-4-semialdehyde) and C3 (dihydroxyacetone phosphate) primary metabolites, whereas most aromatic compounds, including aromatic amino acids, are formed in a multistep reaction via the shikimate pathway	Streptomyces griseus

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Release 2023.1 (January 2023)