BRENDA - Enzyme Database show
show all sequences of 4.1.99.2

Conversion of tyrosine phenol-lyase to dicarboxylic amino acid beta-lyase, an enzyme not found in nature

Mouratou, B.; Kasper, P.; Gehring, H.; Christen, P.; J. Biol. Chem. 274, 1320-1325 (1999)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
R100T/V283R
increases the beta-elimination activity towards dicarboxylic amino acids, L-Asp, L-Glu and L-2-aminoadipate at least 10000-fold
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
L-Tyr
wild-type enzyme
Escherichia coli
0.21
-
L-Asp
mutant enzyme R100T/V283R
Escherichia coli
0.32
-
L-Tyr
mutant enzyme R100T/V283R
Escherichia coli
1.7
-
3-chloro-L-Ala
wild-type enzyme
Escherichia coli
5.3
-
L-Glu
mutant enzyme R100T/V283R
Escherichia coli
46
-
3-chloro-L-Ala
mutant enzyme R100T/V283R
Escherichia coli
54
-
L-Asp
mutant enzyme R100T/V283R
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
SV370; wild-type enzyme, mutant enzyme R100T, mutant enzyme R100T/V283R
-
Escherichia coli SV370
-
SV370
-
Purification (Commentary)
Commentary
Organism
-
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-chloro-L-Ala + H2O
-
33472
Escherichia coli
?
-
-
-
?
3-chloro-L-Ala + H2O
-
33472
Escherichia coli SV370
?
-
-
-
?
L-2-aminoadipate + H2O
no activity with wild-type enzyme, activity with mutant enzyme R100T/V283R
33472
Escherichia coli
?
-
-
-
?
L-2-aminoadipate + H2O
no activity with wild-type enzyme, activity with mutant enzyme R100T/V283R
33472
Escherichia coli SV370
?
-
-
-
?
L-Asp + H2O
no activity with wild-type enzyme, activity with mutant enzyme R100T/V283R
33472
Escherichia coli
formate + pyruvate + NH3
-
33472
Escherichia coli
?
L-Asp + H2O
no activity with wild-type enzyme, activity with mutant enzyme R100T/V283R
33472
Escherichia coli SV370
formate + pyruvate + NH3
-
33472
Escherichia coli SV370
?
L-Glu + H2O
no activity with wild-type enzyme, activity with mutant enzyme R100T/V283R
33472
Escherichia coli
?
-
-
-
?
L-Glu + H2O
no activity with wild-type enzyme, activity with mutant enzyme R100T/V283R
33472
Escherichia coli SV370
?
-
-
-
?
Tyr + H2O
-
33472
Escherichia coli
phenol + pyruvate + NH3
-
-
-
-
Tyr + H2O
-
33472
Escherichia coli SV370
phenol + pyruvate + NH3
-
-
-
-
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.11
-
L-Tyr
mutant enzyme R100T/V283R
Escherichia coli
1.13
-
3-chloro-L-Ala
mutant enzyme R100T/V283R
Escherichia coli
3
-
3-chloro-L-Ala
wild-type enzyme
Escherichia coli
3.7
-
L-Tyr
-
Escherichia coli
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
R100T/V283R
increases the beta-elimination activity towards dicarboxylic amino acids, L-Asp, L-Glu and L-2-aminoadipate at least 10000-fold
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
L-Tyr
wild-type enzyme
Escherichia coli
0.21
-
L-Asp
mutant enzyme R100T/V283R
Escherichia coli
0.32
-
L-Tyr
mutant enzyme R100T/V283R
Escherichia coli
1.7
-
3-chloro-L-Ala
wild-type enzyme
Escherichia coli
5.3
-
L-Glu
mutant enzyme R100T/V283R
Escherichia coli
46
-
3-chloro-L-Ala
mutant enzyme R100T/V283R
Escherichia coli
54
-
L-Asp
mutant enzyme R100T/V283R
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-chloro-L-Ala + H2O
-
33472
Escherichia coli
?
-
-
-
?
3-chloro-L-Ala + H2O
-
33472
Escherichia coli SV370
?
-
-
-
?
L-2-aminoadipate + H2O
no activity with wild-type enzyme, activity with mutant enzyme R100T/V283R
33472
Escherichia coli
?
-
-
-
?
L-2-aminoadipate + H2O
no activity with wild-type enzyme, activity with mutant enzyme R100T/V283R
33472
Escherichia coli SV370
?
-
-
-
?
L-Asp + H2O
no activity with wild-type enzyme, activity with mutant enzyme R100T/V283R
33472
Escherichia coli
formate + pyruvate + NH3
-
33472
Escherichia coli
?
L-Asp + H2O
no activity with wild-type enzyme, activity with mutant enzyme R100T/V283R
33472
Escherichia coli SV370
formate + pyruvate + NH3
-
33472
Escherichia coli SV370
?
L-Glu + H2O
no activity with wild-type enzyme, activity with mutant enzyme R100T/V283R
33472
Escherichia coli
?
-
-
-
?
L-Glu + H2O
no activity with wild-type enzyme, activity with mutant enzyme R100T/V283R
33472
Escherichia coli SV370
?
-
-
-
?
Tyr + H2O
-
33472
Escherichia coli
phenol + pyruvate + NH3
-
-
-
-
Tyr + H2O
-
33472
Escherichia coli SV370
phenol + pyruvate + NH3
-
-
-
-
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.11
-
L-Tyr
mutant enzyme R100T/V283R
Escherichia coli
1.13
-
3-chloro-L-Ala
mutant enzyme R100T/V283R
Escherichia coli
3
-
3-chloro-L-Ala
wild-type enzyme
Escherichia coli
3.7
-
L-Tyr
-
Escherichia coli
Other publictions for EC 4.1.99.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747293
Tang
Process development for effic ...
Fusobacterium nucleatum subsp. nucleatum, Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131
Bioprocess Biosyst. Eng.
41
1347-1354
2018
-
1
1
-
1
-
-
-
-
-
-
2
-
5
-
-
1
-
-
-
-
-
4
-
1
-
-
-
1
-
-
1
-
-
-
-
1
1
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
747513
Kim
Genetic incorporation of l-di ...
Citrobacter freundii, Citrobacter freundii ATCC 8090
Chem. Commun. (Camb.)
54
3002-3005
2018
-
1
1
-
1
-
-
-
-
-
-
2
-
3
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
1
1
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
747667
Zheng
Biochemical characterization ...
Fusobacterium nucleatum subsp. nucleatum, Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131
Enzyme Microb. Technol.
112
88-93
2018
1
1
1
-
-
-
-
4
-
-
-
2
-
5
-
-
1
-
-
-
-
-
17
-
1
1
-
4
1
1
-
1
-
-
-
1
1
1
1
-
-
-
-
-
-
4
-
-
-
2
-
-
-
1
-
-
-
-
17
-
1
1
-
4
1
1
-
-
-
-
-
-
3
3
746580
Phillips
-
Ground-state destabilization ...
Citrobacter freundii
ACS Catal.
6
6770-6779
2016
-
-
1
-
5
-
-
1
-
-
-
1
-
1
-
-
1
1
-
-
-
-
6
1
1
-
-
15
1
-
-
1
-
-
-
-
-
1
1
-
5
-
-
-
-
1
-
-
-
1
-
-
-
1
-
-
-
-
6
1
1
-
-
15
1
-
-
-
-
-
-
-
15
15
746722
Do
Inhibition of tyrosine phenol ...
Citrobacter freundii
Amino Acids
48
2243-2251
2016
-
-
1
-
-
-
5
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
2
-
1
-
-
-
1
-
-
1
5
-
-
-
-
1
1
-
-
-
-
5
5
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
746892
Miao
Construction of a novel pheno ...
Citrobacter freundii
Appl. Microbiol. Biotechnol.
99
5163-5173
2015
-
-
-
-
1
-
3
15
-
-
-
1
-
3
-
-
-
1
-
-
-
-
8
-
1
-
-
14
1
-
-
1
1
-
-
-
-
-
1
-
1
-
-
3
1
15
-
-
-
1
-
-
-
-
-
-
-
-
8
-
1
-
-
14
1
-
-
-
-
1
1
-
14
14
727217
Kim
Metabolic engineering of Esche ...
Escherichia coli
Biotechnol. J.
9
621-629
2014
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
747245
Phillips
The role of substrate strain ...
Citrobacter freundii
Bioorg. Chem.
57
198-205
2014
-
-
-
1
5
-
1
1
-
1
-
1
-
1
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
5
-
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
726642
Azmi
Paraffin as oxygen vector modu ...
Citrobacter freundii, Citrobacter freundii MTCC 2424
Acta Microbiol. Immunol. Hung.
60
145-154
2013
-
1
-
-
-
-
-
-
-
-
-
4
-
4
-
-
-
-
-
2
-
-
4
-
1
-
-
-
1
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
2
-
-
4
-
1
-
-
-
1
-
-
-
1
-
-
1
-
-
726700
Chandel
Purification and characterizat ...
Citrobacter freundii
Appl. Biochem. Biotechnol.
171
2040-2052
2013
-
1
-
-
-
-
-
1
2
-
2
2
-
2
-
-
1
-
-
-
1
-
7
2
1
1
3
-
1
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
1
2
-
2
2
-
-
-
1
-
-
1
-
7
2
1
1
3
-
1
-
-
-
-
1
1
-
-
-
727663
Milic
Crystallographic snapshots of ...
Citrobacter freundii
J. Am. Chem. Soc.
133
16468-16476
2011
-
-
1
1
3
-
-
-
-
-
-
1
-
2
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1
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4
1
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1
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1
1
1
3
-
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1
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-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
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713693
Seisser
-
Cutting long syntheses short: ...
Citrobacter freundii
Adv. Synth. Catal.
352
731-736
2010
-
-
1
-
5
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
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9
-
-
-
-
-
1
-
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1
-
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1
1
-
5
-
-
-
-
-
-
-
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1
-
-
-
-
-
-
-
-
9
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
713730
Koulikova
Stereospecificity of isotopic ...
Citrobacter freundii
Amino Acids
41
1247-1256
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
1
-
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-
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1
-
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-
-
1
-
-
-
-
-
-
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-
-
-
-
-
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1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
691349
Chandel
Optimization of process parame ...
Citrobacter freundii, Citrobacter freundii MTCC 2424
Biores. Technol.
100
1840-1846
2009
-
1
-
-
-
-
-
-
-
-
-
2
-
5
-
-
-
-
-
-
-
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2
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1
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1
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1
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2
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-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
702744
Koyanagi
Hyperproduction of 3,4-dihydro ...
Pantoea agglomerans
Biosci. Biotechnol. Biochem.
73
1221-1223
2009
-
-
-
-
-
-
-
-
-
-
-
-
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3
-
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-
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2
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2
-
-
-
-
-
-
-
-
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1
1
1
1
-
-
702808
Kurt
Production of L-DOPA and dopam ...
Citrobacter freundii, Citrobacter freundii NRRL B-2643, Pantoea agglomerans, Pantoea agglomerans NRRL B-3466
Biotechnol. J.
4
1077-1088
2009
2
-
-
-
-
-
-
-
-
-
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9
-
-
-
-
-
-
9
-
4
-
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2
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9
-
4
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
703665
Rha
Simultaneous improvement of ca ...
Symbiobacterium toebii
FEBS J.
276
6187-6194
2009
-
-
1
-
14
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
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-
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-
-
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1
-
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14
-
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1
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1
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-
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-
692854
Wierckx
Transcriptome analysis of a ph ...
Pseudomonas putida
J. Bacteriol.
190
2822-2830
2008
-
-
1
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1
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1
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3
-
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1
4
-
1
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1
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1
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1
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1
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1
4
-
1
-
1
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-
693155
Milic
Insights into the catalytic me ...
Citrobacter freundii
J. Biol. Chem.
283
29206-29214
2008
-
-
1
1
-
-
-
-
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Ionization state of pyridoxal ...
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678198
Milic
Structures of apo- and holo-ty ...
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678212
Phillips
Aminoacrylate intermediates in ...
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Aspartic acid 214 in Citrobact ...
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664018
Phillips
Role of lysine-256 in Citrobac ...
Citrobacter freundii
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The mechanism of alpha-proton ...
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Effects of cyclodextrin deriva ...
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Phillips
Structure and mechanism of try ...
Citrobacter freundii
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Faleev
The role of acidic dissociatio ...
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Demidkina
Threonine-124 and phenylalanin ...
Citrobacter freundii
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Phillips
Crystals of tryptophan indole- ...
Citrobacter freundii
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650039
Watkins
Inhibition of tyrosine phenol- ...
Citrobacter freundii
Biochemistry
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651062
Bazhulina
Pyridoxal 5'-phoshate schiff b ...
Citrobacter freundii
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651086
Faleev
Interaction of tyrosine phenol ...
Citrobacter intermedius
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653702
Barbolina
Citrobacter freundii tyrosine ...
Citrobacter freundii
Protein Eng.
13
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Conversion of tyrosine phenol- ...
Escherichia coli, Escherichia coli SV370
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Yamada
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Screening of novel enzymes for ...
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33468
Lee
Thermostable tyrosine phenol-l ...
Symbiobacterium sp., Symbiobacterium sp. SC-1
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11
263-270
1997
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33470
Sundaraju
The crystal structure of Citro ...
Citrobacter freundii
Biochemistry
36
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1997
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Lee
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33461
Pletnev
Purification and crystals of t ...
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33467
Faleev
The catalytic mechanism of tyr ...
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1996
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33462
Chen
Site-directed mutagenesis of H ...
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Chen
Site-directed mutagenesis of t ...
Citrobacter freundii
Biochemistry
34
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33463
Foor
Production of L-dihydroxypheny ...
Pantoea agglomerans
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59
3070-3075
1993
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1
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33464
Hirahara
Cloning, nucleotide sequence, ...
Symbiobacterium thermophilum
Appl. Microbiol. Biotechnol.
39
341-346
1993
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1
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1
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4
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1
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33469
Chen
Binding of phenol and analogue ...
Citrobacter freundii
Biochemistry
32
11591-11599
1993
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1
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2
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1
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1
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33465
Suzuki
-
Purification and properties of ...
Symbiobacterium thermophilum
Biosci. Biotechnol. Biochem.
56
84-89
1992
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-
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3
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2
2
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1
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1
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1
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7
1
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1
3
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1
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1
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2
2
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1
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1
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7
1
1
1
3
-
1
-
1
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-
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33466
Anderson
Development of a multienzyme r ...
Pantoea agglomerans
Biotechnol. Bioeng.
39
781-789
1992
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1
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3
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3
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3
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33441
Kiick
Mechanistic deductions from ki ...
Citrobacter freundii, Pantoea agglomerans
Biochemistry
27
7333-7338
1988
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5
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2
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-
2
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33442
Demidkina
Crystallization and crystal da ...
Citrobacter freundii
FEBS Lett.
232
381-382
1988
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1
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33443
Demidkina
Transamination catalysed by ty ...
Citrobacter intermedius
Eur. J. Biochem.
170
311-316
1987
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3
6
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2
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6
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6
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6
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6
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33444
Palice
Stereochemistry and mechanism ...
Citrobacter intermedius
Z. Naturforsch. C
42
307-318
1987
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3
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33445
Nagasawa
-
Location of tyrosine phenol-ly ...
Citrobacter intermedius, Escherichia coli, Morganella morganii
FEMS Microbiol. Lett.
30
269-272
1985
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3
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33446
Pierson
Properties of stearylamine lip ...
Pantoea agglomerans
Biochim. Biophys. Acta
676
177-186
1981
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1
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33447
Nagasawa
Syntheses of L-tyrosine-relate ...
Citrobacter intermedius
Eur. J. Biochem.
117
33-40
1981
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11
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3
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1
1
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1
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21
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1
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1
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-
17
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11
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1
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1
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21
-
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33448
Faleev
-
Catalytic properties of extrac ...
Citrobacter intermedius
Enzyme Microb. Technol.
3
219-224
1981
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2
1
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1
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1
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2
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1
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2
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33449
McKenzie
-
The periplasmic location of ty ...
Pantoea agglomerans
FEMS Microbiol. Lett.
9
255-258
1980
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33450
Faleev
-
Reactions of partially purifie ...
Citrobacter intermedius
Enzyme Microb. Technol.
1
269-272
1979
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1
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1
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7
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1
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1
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1
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7
-
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33451
Muro
Elementary processes in the in ...
Citrobacter intermedius
J. Biochem.
84
633-640
1978
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1
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33452
Carman
Partial purification and some ...
Aeromonas phenologenes
Appl. Environ. Microbiol.
33
192-198
1977
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1
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1
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1
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1
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1
1
1
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4
-
1
1
-
1
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1
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1
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1
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-
1
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1
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1
1
1
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-
4
-
1
1
-
-
-
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-
-
-
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33453
Yamada
Synthesis of L-tyrosine-relate ...
Citrobacter intermedius, Pantoea agglomerans
Adv. Appl. Microbiol.
19
249-288
1975
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1
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2
2
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2
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1
2
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2
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-
2
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2
1
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2
-
2
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1
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-
2
-
-
-
-
-
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-
-
-
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-
-
-
-
33454
Enei
-
Elimination, replacement and i ...
Pantoea agglomerans, Pantoea agglomerans ATCC 21434
Agric. Biol. Chem.
36
1869-1876
1972
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2
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15
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-
15
-
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-
-
-
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-
-
-
-
33455
Enei
-
Distribution of tyrosine pheno ...
Bacillus cereus, Citrobacter freundii, Citrobacter intermedius, Escherichia coli, Klebsiella aerogenes, Morganella morganii, Pantoea agglomerans, Pseudomonas pelurida, Pseudomonas trifolii, Salmonella enterica subsp. enterica serovar Typhimurium, Xanthomonas campestris
Agric. Biol. Chem.
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1861-1868
1972
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11
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1
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20
-
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1
-
20
-
-
-
-
-
-
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33456
Kumagai
-
Purification, crystallization ...
Pantoea agglomerans
Agric. Biol. Chem.
36
472-482
1972
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1
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4
2
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1
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1
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-
1
-
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3
-
-
-
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-
1
-
-
1
-
-
-
-
-
-
1
1
-
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-
4
-
2
-
-
1
-
-
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-
1
-
-
-
-
3
-
-
-
-
-
1
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-
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33457
Yamada
Synthesis of L-tyrosine from p ...
Citrobacter intermedius
Biochem. Biophys. Res. Commun.
46
370-374
1972
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2
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1
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1
-
3
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-
1
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2
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1
-
3
-
-
-
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-
1
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33458
Kumagai
Tyrosine phenol lyase. I. Puri ...
Citrobacter intermedius
J. Biol. Chem.
245
1767-1772
1970
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1
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1
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5
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-
2
-
1
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-
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-
-
-
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-
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-
1
-
-
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1
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5
-
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-
2
-
1
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33459
Kumagai
Tyrosine phenol lyase. II. Cof ...
Citrobacter intermedius
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245
1773-1777
1970
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-
1
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-
1
-
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-
1
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-
-
2
-
-
-
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