Literature summary for 4.1.99.17 extracted from

Palmer, L.D.; Paxhia, M.D.; Downs, D.M.
Induction of the sugar-phosphate stress response allows Saccharomyces cerevisiae 2-methyl-4-amino-5-hydroxymethylpyrimidine phosphate synthase to function in Salmonella enterica (2015), J. Bacteriol., 197, 3554-3562 .

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Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	one of the mechanisms of Thi5p activation requires decreased PtsG function and an undefined role of SgrR	Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment)	Organism
gene YFL058w, recombinant expression of GST-tagged enzyme mutant in Salmonella enterica strain LT2, one mechanism to allow Thi5p function in Salmonella enterica is by remodeling the metabolic network associated with the sugar phosphate stress response regulator, transcription factor SgrR (formerly YabN), integration between the sugar-phosphate stress response regulator and Thi5p activity in Salmonella enterica. SgrR belongs to a distinct class of transcription regulators (COG4533) and has a predicted N-terminal DNA-binding domain and C-terminal solute-binding domain, expression from the sgrS promoter (sgrSp) is used as a reporter of SgrR activity. Thi5p must be activated in order to function in Salmonella enterica, possibly by phosphorylation or another posttranslational modification	Saccharomyces cerevisiae

Cloned (Comment)

Organism

gene YFL058w, recombinant expression of GST-tagged enzyme mutant in Salmonella enterica strain LT2, one mechanism to allow Thi5p function in Salmonella enterica is by remodeling the metabolic network associated with the sugar phosphate stress response regulator, transcription factor SgrR (formerly YabN), integration between the sugar-phosphate stress response regulator and Thi5p activity in Salmonella enterica. SgrR belongs to a distinct class of transcription regulators (COG4533) and has a predicted N-terminal DNA-binding domain and C-terminal solute-binding domain, expression from the sgrS promoter (sgrSp) is used as a reporter of SgrR activity. Thi5p must be activated in order to function in Salmonella enterica, possibly by phosphorylation or another posttranslational modification

Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
M37I/A138V/G152D	site-directed mutagenesis	Saccharomyces cerevisiae
additional information	disruption of glycolysis allows Thi5p-dependent growth on glucose. Generation of an insertion mutation in ptsG allowing Thi5p-dependent thiamine synthesis in a DELTAthiC strain, the effect of the ptsG mutation is independent of sgrS, consistent with a role for sgrS in decreasing PtsG activity. Induction of the sugar-phosphate stress response is required to decrease PtsG activity. Disrupting ptsG independently restores thiamine synthesis revealing one mechanism connecting Thi5p function and induction of the sugar-phosphate stress response	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine	Saccharomyces cerevisiae	-	4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO	-	?
5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine	Saccharomyces cerevisiae ATCC 204508 / S288c	-	4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	P43534	-	-
Saccharomyces cerevisiae ATCC 204508 / S288c	P43534	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine	-	Saccharomyces cerevisiae	4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO	-	?
5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine	-	Saccharomyces cerevisiae ATCC 204508 / S288c	4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO	-	?

Synonyms

Synonyms	Comment	Organism
2-methyl-4-amino-5-hydroxymethylpyrimidine phosphate synthase	-	Saccharomyces cerevisiae
HMP-P synthase	-	Saccharomyces cerevisiae
Thi5p	-	Saccharomyces cerevisiae

Cofactor

Cofactor	Comment	Organism	Structure
S-adenosyl-L-methionine	-	Saccharomyces cerevisiae

General Information

General Information	Comment	Organism
malfunction	one mechanism to allow Thi5p function in Salmonella enterica is by remodeling the metabolic network associated with the sugar phosphate stress response regulator, transcription factor SgrR (formerly YabN), integration between the sugar-phosphate stress response regulator and Thi5p activity in Salmonella enterica. SgrR belongs to a distinct class of transcription regulators (COG4533) and has a predicted N-terminal DNA-binding domain and C-terminal solute-binding domain, expression from the sgrS promoter (sgrSp) is used as a reporter of SgrR activity	Saccharomyces cerevisiae
metabolism	one of the mechanisms of Thi5p activation requires decreased PtsG function and an undefined role of SgrR	Saccharomyces cerevisiae