Cloned (Comment) | Organism |
---|---|
gene thiC, recombinant expression of His-tagged N-terminally truncated mutant DELTAN71-AtTHIC in Escherichia coli strain BL21(DE3) | Arabidopsis thaliana |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged DELTAN71-AtTHIC mutant, by a proteolysis assay (especially alpha-chymotrypsin) coupled with the sitting drop vapor diffusion technique at 18°C, using 0.01 M cobalt (II) chloride hexahydrate, 0.1 M sodium acetate trihydrate, pH 4.6, 1 M 1,6-hexanediol, X-ray diffraction structure determination and analysis at 1.6 A resolution | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of an N-terminally truncated version of Arabidopsis thaliana THIC, lacking the first 71 amino acids, a chloroplastidial targeting peptide. The truncated AtTHIC is functionally active | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | a metal ion with octahedral coordination (two strictly conserved histidine residues (H426 and H490) and four water molecules) at the same location as a zinc ion in the bacterial enzyme, and a metal ion with multiple coordinated water molecules in the close vicinity of the substrate binding sites, binding structures, overview | Arabidopsis thaliana | |
Fe2+ | the enzyme contains a [4Fe-4S] cluster | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65451 | - |
2 * 65451, recombinant His-tagged DELTAN71-AtTHIC mutant, mass spectrometry and crystal structure | Arabidopsis thaliana |
130000 | - |
recombinant His-tagged DELTAN71-AtTHIC mutant, gel filtration | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine | Arabidopsis thaliana | - |
4-amino-2-methyl-5-phosphomethylpyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | O82392 | gene thiC | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged N-terminally truncated mutant DELTAN71-AtTHIC from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine | - |
Arabidopsis thaliana | 4-amino-2-methyl-5-phosphomethylpyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO | - |
? | |
5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine | the enzyme uses an iron-sulfur cluster as well as a 5'-deoxyadenosyl radical as cofactors to rearrange the 5-amino-imidazole ribonucleotide (AIR) substrate to the pyrimidine ring | Arabidopsis thaliana | 4-amino-2-methyl-5-phosphomethylpyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 65451, recombinant His-tagged DELTAN71-AtTHIC mutant, mass spectrometry and crystal structure | Arabidopsis thaliana |
More | enzyme three-dimensional structure analysis, overview | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
HMP-P synthase | - |
Arabidopsis thaliana |
thiC | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | a S-adenosyl-L-methionine radical enzyme | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is important in thiamine diphosphate, vitamin B1, biosynthesis, an essential cofactor for key cellular metabolic enzymes in all forms of life | Arabidopsis thaliana |
additional information | enzyme three-dimensional structure analysis and comparison, overview | Arabidopsis thaliana |