Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.99.17 extracted from

  • Chatterjee, A.; Li, Y.; Zhang, Y.; Grove, T.L.; Lee, M.; Krebs, C.; Booker, S.J.; Begley, T.P.; Ealick, S.E.
    Reconstitution of ThiC in thiamine pyrimidine biosynthesis expands the radical SAM superfamily (2008), Nat. Chem. Biol., 4, 758-765.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.99.17

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Caulobacter vibrioides

Crystallization (Commentary)

Crystallization (Comment) Organism
selenomethionine HMP-P synthase/HMP crystals are grown using the sitting drop vapor diffusion method at 25°C. The crystals are monoclinic, space group P2(1), with unit cell dimension a = 63.3 A, b = 103.4 A, c = 95.4 A and beta = 91.6° Caulobacter vibrioides

Metals/Ions

Metals/Ions Comment Organism Structure
4Fe-4S center binds 1 4Fe-4S cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine Caulobacter vibrioides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine Caulobacter vibrioides the enzyme is involved in the biosynthesis of thiamine diphosphate 4-amino-2-methyl-5-phosphomethylpyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO
-
 ?

Organism

Organism UniProt Comment Textmining
Caulobacter vibrioides Q9A6Q5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine
-
 Caulobacter vibrioides 4-amino-2-methyl-5-phosphomethylpyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO
-
 ?
5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine the enzyme is involved in the biosynthesis of thiamine diphosphate Caulobacter vibrioides 4-amino-2-methyl-5-phosphomethylpyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO
-
 ?

Subunits

Subunits Comment Organism
homodimer
-
 Caulobacter vibrioides

Synonyms

Synonyms Comment Organism
4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase
-
 Caulobacter vibrioides
HMP-P synthase
-
 Caulobacter vibrioides
thiC
-
 Caulobacter vibrioides

Cofactor

Cofactor Comment Organism Structure
[4Fe-4S]-center binds 1 4Fe-4S cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine Caulobacter vibrioides

General Information

General Information Comment Organism
physiological function the enzyme is involved in the biosynthesis of thiamine diphosphate Caulobacter vibrioides