Cloned (Comment) | Organism |
---|---|
gene geo, cloning of full-length of geosmin synthase gene from Aphanizomenon gracile strain WH-1, DNA and amino acid sequence determination and analysis, phylogenetic tree, real time PCR is applied to quantify the geosmin production in different light and temperature. Low temperature (15°C), high light intensity (0.035 mmol/s/m) and continuous light illumination are beneficial to the expression of geo. The successful amplification of geosmin synthase gene verifies that hiTAIL-PCR is an effective and simple procedure of low cost | Aphanizomenon gracile |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + H2O | Aphanizomenon gracile | - |
(-)-geosmin + acetone | - |
? | |
(1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + H2O | Aphanizomenon gracile WH-1 | - |
(-)-geosmin + acetone | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aphanizomenon gracile | A0A0D5W9R1 | - |
- |
Aphanizomenon gracile WH-1 | A0A0D5W9R1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + H2O | - |
Aphanizomenon gracile | (-)-geosmin + acetone | - |
? | |
(1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + H2O | - |
Aphanizomenon gracile WH-1 | (-)-geosmin + acetone | - |
? |
Synonyms | Comment | Organism |
---|---|---|
geo | - |
Aphanizomenon gracile |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is responsible for geosmin biosynthesis. Geosmin is a secondary metabolite responsible for earthy odors. The occurrence of geosmin has great impact on the quality of water environment. Analysis of the geosmin production under d ifferent environments | Aphanizomenon gracile |