BRENDA - Enzyme Database
show all sequences of 4.1.99.16

Cloning and expression analysis of geosmin synthase gene from Aphanizomenon gracile

Zhou, W.; Li, G.; Gao, X.; Dai, L.; Guo, L.; Wang, S.; Chin. J. Appl. Environ. Biol. 21, 824-829 (2015)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene geo, cloning of full-length of geosmin synthase gene from Aphanizomenon gracile strain WH-1, DNA and amino acid sequence determination and analysis, phylogenetic tree, real time PCR is applied to quantify the geosmin production in different light and temperature. Low temperature (15C), high light intensity (0.035 mmol/s/m) and continuous light illumination are beneficial to the expression of geo. The successful amplification of geosmin synthase gene verifies that hiTAIL-PCR is an effective and simple procedure of low cost
Aphanizomenon gracile
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + H2O
Aphanizomenon gracile
-
(-)-geosmin + acetone
-
-
?
(1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + H2O
Aphanizomenon gracile WH-1
-
(-)-geosmin + acetone
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Aphanizomenon gracile
A0A0D5W9R1
-
-
Aphanizomenon gracile WH-1
A0A0D5W9R1
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + H2O
-
747590
Aphanizomenon gracile
(-)-geosmin + acetone
-
-
-
?
(1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + H2O
-
747590
Aphanizomenon gracile WH-1
(-)-geosmin + acetone
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
geo
-
Aphanizomenon gracile
Cloned(Commentary) (protein specific)
Commentary
Organism
gene geo, cloning of full-length of geosmin synthase gene from Aphanizomenon gracile strain WH-1, DNA and amino acid sequence determination and analysis, phylogenetic tree, real time PCR is applied to quantify the geosmin production in different light and temperature. Low temperature (15C), high light intensity (0.035 mmol/s/m) and continuous light illumination are beneficial to the expression of geo. The successful amplification of geosmin synthase gene verifies that hiTAIL-PCR is an effective and simple procedure of low cost
Aphanizomenon gracile
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + H2O
Aphanizomenon gracile
-
(-)-geosmin + acetone
-
-
?
(1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + H2O
Aphanizomenon gracile WH-1
-
(-)-geosmin + acetone
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + H2O
-
747590
Aphanizomenon gracile
(-)-geosmin + acetone
-
-
-
?
(1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + H2O
-
747590
Aphanizomenon gracile WH-1
(-)-geosmin + acetone
-
-
-
?
General Information
General Information
Commentary
Organism
physiological function
the enzyme is responsible for geosmin biosynthesis. Geosmin is a secondary metabolite responsible for earthy odors. The occurrence of geosmin has great impact on the quality of water environment. Analysis of the geosmin production under d ifferent environments
Aphanizomenon gracile
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the enzyme is responsible for geosmin biosynthesis. Geosmin is a secondary metabolite responsible for earthy odors. The occurrence of geosmin has great impact on the quality of water environment. Analysis of the geosmin production under d ifferent environments
Aphanizomenon gracile
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743387
Schifrin
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3385-3393
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747590
Zhou
-
Cloning and expression analys ...
Aphanizomenon gracile, Aphanizomenon gracile WH-1
Chin. J. Appl. Environ. Biol.
21
824-829
2015
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1
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728186
Giglio
Expression of the geosmin synt ...
Dolichospermum circinale, Dolichospermum circinale AWQC318
J. Phycol.
47
1338-1343
2011
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1
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4
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712642
Singh
Exploration of geosmin synthas ...
Streptomyces peucetius, Streptomyces peucetius ATCC 27952
J. Ind. Microbiol. Biotechnol.
36
1257-1265
2009
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6
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1
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692761
Jiang
Geosmin biosynthesis. Mechanis ...
Streptomyces coelicolor
J. Am. Chem. Soc.
130
428-429
2008
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680396
Cane
Geosmin biosynthesis in Strept ...
Streptomyces avermitilis
J. Antibiot.
59
471-479
2006
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7
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7
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