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Literature summary for 4.1.99.14 extracted from
- High-pressure liquid chromatography assay for quantitatively monitoring spore photoproduct repair mediated by spore photoproduct lyase during germination of uv-irradiated Bacillus subtilis spores (1994), Anal. Biochem., 221, 61-65.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | a rapid separation technique for detecting and quantitating SP by chromatography : tritiated thymine-containing photoproducts from trifluoroacetic acid-hydrolyzed DNA purified from UV-irradiated cells or spores of Bacillus subtilis are identified and isolated from paper chromatograms, subjected to HPLC on a Microsorb phenyl 5-micrometer column using 100% water as the mobile phase, and detected by scintillation counting of collected fractions | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-thyminyl-5,6 dihydrothymine + S-adenosyl-L-methionine | - |
Bacillus subtilis | thymidylyl-(3'-5')-thymidylate + 5'-deoxyadenosine + L-methionine | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SP lyase | - |
Bacillus subtilis |
| SPL | - |
Clostridium acetobutylicum |
| spore photoproduct lyase | - |
Bacillus subtilis |
| spore photoproduct lyase | - |
Clostridium acetobutylicum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| S-adenosyl-L-methionine | - |
Bacillus subtilis | |
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