Any feedback?
Literature summary for 4.1.99.13 extracted from
- Purification and characterization of five members of photolyase/cryptochrome family from Cyanidioschyzon merolae (2012), Plant Sci., 185-186, 190-198.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| phylogenetic analysis, DNA and amino acid sequence determination and analysis, expression of isozymes in Escherichia coli strain UNC 523 from pMal-c2x vector | Cyanidioschyzon merolae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (6-4) photoproduct (in DNA) | Cyanidioschyzon merolae | (6-4) photolyases repair (6-4) pyrimidine-pyrimidone photoproducts. CmPHR1 exhibits a repair activity of both (6-4)-ssDNA and (6-4)-dsDNA. Cryptochromes CmPHR2 and CmPHR5 show CPD repair activity only on ssDNA, and have no repair activity when CPD-damaged dsDNA is used as a substrate | 2 pyrimidine residues (in DNA) | - |
? |  |
| (6-4) photoproduct (in DNA) | Cyanidioschyzon merolae 10D | (6-4) photolyases repair (6-4) pyrimidine-pyrimidone photoproducts. CmPHR1 exhibits a repair activity of both (6-4)-ssDNA and (6-4)-dsDNA. Cryptochromes CmPHR2 and CmPHR5 show CPD repair activity only on ssDNA, and have no repair activity when CPD-damaged dsDNA is used as a substrate | 2 pyrimidine residues (in DNA) | - |
? | |
| additional information | Cyanidioschyzon merolae | the repair function requires to transfer a functional group (OH in the case T(6-4)T) from the 5' to the 3' base, in addition to intradimer bond cleavage | ? | - |
? | |
| additional information | Cyanidioschyzon merolae 10D | the repair function requires to transfer a functional group (OH in the case T(6-4)T) from the 5' to the 3' base, in addition to intradimer bond cleavage | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cyanidioschyzon merolae | M1V623 | CmPHR1; gene CmPHR1 | - |
| Cyanidioschyzon merolae 10D | M1V623 | CmPHR1; gene CmPHR1 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (6-4) photoproduct (in DNA) | (6-4) photolyases repair (6-4) pyrimidine-pyrimidone photoproducts. CmPHR1 exhibits a repair activity of both (6-4)-ssDNA and (6-4)-dsDNA. Cryptochromes CmPHR2 and CmPHR5 show CPD repair activity only on ssDNA, and have no repair activity when CPD-damaged dsDNA is used as a substrate | Cyanidioschyzon merolae | 2 pyrimidine residues (in DNA) | - |
? | |
| (6-4) photoproduct (in DNA) | substrate is radio-labeled 48-bp DNA substrates that contains CPD or (6-4) damages within MseI restriction sites (TTAA), pyrimidine(6-4)pyrimidone photoptoducts | Cyanidioschyzon merolae | 2 pyrimidine residues (in DNA) | - |
? | |
| (6-4) photoproduct (in DNA) | (6-4) photolyases repair (6-4) pyrimidine-pyrimidone photoproducts. CmPHR1 exhibits a repair activity of both (6-4)-ssDNA and (6-4)-dsDNA. Cryptochromes CmPHR2 and CmPHR5 show CPD repair activity only on ssDNA, and have no repair activity when CPD-damaged dsDNA is used as a substrate | Cyanidioschyzon merolae 10D | 2 pyrimidine residues (in DNA) | - |
? | |
| (6-4) photoproduct (in DNA) | substrate is radio-labeled 48-bp DNA substrates that contains CPD or (6-4) damages within MseI restriction sites (TTAA), pyrimidine(6-4)pyrimidone photoptoducts | Cyanidioschyzon merolae 10D | 2 pyrimidine residues (in DNA) | - |
? | |
| additional information | the repair function requires to transfer a functional group (OH in the case T(6-4)T) from the 5' to the 3' base, in addition to intradimer bond cleavage | Cyanidioschyzon merolae | ? | - |
? | |
| additional information | the repair function requires to transfer a functional group (OH in the case T(6-4)T) from the 5' to the 3' base, in addition to intradimer bond cleavage | Cyanidioschyzon merolae 10D | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| (6-4) photolyase | - |
Cyanidioschyzon merolae |
| CmPHR1 | - |
Cyanidioschyzon merolae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at room temperature | Cyanidioschyzon merolae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Cyanidioschyzon merolae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 5,10-methenyltetrahydrofolate | required | Cyanidioschyzon merolae | |
| FAD | an essential cofactor, the enzyme contains FAD at a concentration of less than 0.2% | Cyanidioschyzon merolae | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the photolyase/cryptochrome family, a large family of flavoproteins that possess different functions and use blue light as an energy source, phylogenetic analysis. Of the seven members of this gene family, three (CmPHR2, CmPHR5 and CmPHR6) fall within the clade of cryptochrome DASH, three (CmPHR3, CmPHR4 and CmPHR7) group with plant cryptochromes, and one (CmPHR1) is a homologue of (6-4) photolyase. Photolyases repair UV-induced DNA damage, whereas cryptochromes regulate the growth and development of plants in a blue-light dependent manner | Cyanidioschyzon merolae |
| physiological function | the photolyase repairs UV-induced DNA damage | Cyanidioschyzon merolae |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
