Literature summary for 4.1.99.13 extracted from

Li, J.; Liu, Z.; Tan, C.; Guo, X.; Wang, L.; Sancar, A.; Zhong, D.
Dynamics and mechanism of repair of ultraviolet-induced (6-4) photoproduct by photolyase (2010), Nature, 466, 887-890.

Search for more literature for 4.1.99.13

Cloned(Commentary)

Cloned (Comment)	Organism
expressed as a His-tagged fusion protein	Arabidopsis thaliana

Protein Variants

Protein Variants	Comment	Organism
H364A	compared to wild-type mutant similar electron transfer dynamics in the range of 70-260 ps but decay to zero without any long plateaus, H364 is irreplaceable: Steady-state quantum yield measurements reveal a total lack of repair with the mutant	Arabidopsis thaliana
H364D	compared to wild-type mutant similar electron transfer dynamics in the range of 70-260 ps but decay to zero without any long plateaus, H364 is irreplaceable: Steady-state quantum yield measurements reveal a total lack of repair with the mutant	Arabidopsis thaliana
H364K	compared to wild-type mutant similar electron transfer dynamics in the range of 70-260 ps but decay to zero without any long plateaus, H364 is irreplaceable: Steady-state quantum yield measurements reveal a total lack of repair with the mutant	Arabidopsis thaliana
H364M	compared to wild-type mutant similar electron transfer dynamics in the range of 70-260 ps but decay to zero without any long plateaus, H364 is irreplaceable: Steady-state quantum yield measurements reveal a total lack of repair with the mutant	Arabidopsis thaliana
H364N	compared to wild-type mutant similar electron transfer dynamics in the range of 70-260 ps but decay to zero without any long plateaus, H364 is irreplaceable: Steady-state quantum yield measurements reveal a total lack of repair with the mutant	Arabidopsis thaliana
H364Y	compared to wild-type mutant similar electron transfer dynamics in the range of 70-260 ps but decay to zero without any long plateaus, H364 is irreplaceable: Steady-state quantum yield measurements reveal a total lack of repair with the mutant	Arabidopsis thaliana

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Arabidopsis thaliana

Reaction

Reaction	Comment	Organism	Reaction ID
(6-4) photoproduct (in DNA) = 2 pyrimidine residues (in DNA)	ultrafast spectroscopy is used to show that the key step in the repair photocycle is a cyclic proton transfer between the enzyme and the substrate. By femtosecond synchronization of the enzymatic dynamics with the repair function, direct electron transfer from the excited flavin cofactor to the 6-4 photoproduct is observed in 225 ps but fast back electron transfer in 50 ps without repair. The catalytic proton transfer between a histidine residue in the active site and the 6-4 photoproduct, induced by the initial photoinduced electron transfer from the excited flavin cofactor to 6-4 photoproduct, occurs in 425 ps and leads to 6-4 photoproduct repair in tens of nanoseconds	Arabidopsis thaliana	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(6-4) photoproduct (in DNA)	-	Arabidopsis thaliana	2 pyrimidine residues (in DNA)	-	?

Synonyms

Synonyms	Comment	Organism
(6-4) photolyase	-	Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
additional information	-	assay carried out at room temperature	Arabidopsis thaliana

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Arabidopsis thaliana

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Arabidopsis thaliana

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)