Literature summary for 4.1.99.13 extracted from

Todo, T.; Ryo, H.; Borden, A.; Lawrence, C.; Sakaguchi, K.; Hirata, H.; Nomura, T.
Non-mutagenic repair of (6-4)photoproducts by (6-4)photolyase purified from Drosophila melanogaster (1997), Mutat. Res., 385, 83-93.

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Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
62000	-	SDS-PAGE	Drosophila melanogaster

Organism

Organism	UniProt	Comment	Textmining
Drosophila melanogaster	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
purified to near homogeneity from Drosophila embryonic cells by using NH4SO4 precipitation, SP sepharose column, UV DNA affinity beads, heparin sepharose column and Mono S column	Drosophila melanogaster

Reaction

Reaction	Comment	Organism	Reaction ID
(6-4) photoproduct (in DNA) = 2 pyrimidine residues (in DNA)	The overall repair reaction consists of two distinct steps, one of which is light-independent and the other one light-dependent. In the initial light-independent step, a 6-iminium ion is thought to be generated via proton transfer induced by two histidines highly conserved among the (6-4) photolyases.This intermediate spontaneously rearranges to form an oxetane intermediate by intramolecular nucleophilic attack. In the subsequent light-driven reaction, one electron is believed to be transferred from the fully reduced FAD cofactor (FADH-) to the oxetane intermediate thus forming a neutral FADH radical and an anionic oxetane radical, which spontaneously fractures. The excess electron is then back-transferred to the flavin radical restoring the fully reduced flavin cofactor and a pair of pyrimidine bases	Drosophila melanogaster	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
C(6-4)C photoproduct (in DNA)	-	Drosophila melanogaster	2 cytosine residues (in DNA)	-	?
T(6-4)C photoproduct (in DNA)	-	Drosophila melanogaster	2 thymidine + cytosine (in DNA)	-	?
T(6-4)T photoproduct (in DNA)	-	Drosophila melanogaster	2 thymidine resdiues (in DNA)	-	?

Synonyms

Synonyms	Comment	Organism
(6-4) photolyase	-	Drosophila melanogaster

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Drosophila melanogaster

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Release 2023.1 (January 2023)