Cloned (Comment) | Organism |
---|---|
expressed as a GST-tagged fusion protein in Escherichia coli | Dunaliella salina |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme is also active under high salinity | Dunaliella salina | |
additional information | NH4Cl or NaH2PO4 increase activity of the enzyme. CH3COONa or Na2CO3 strongly decrease the enzymes activity. Maximum activity occurs in the presence of NaH2PO4, which is increased eight times than that in the presence of Na2CO3. When the ions that possess stronger ability to donate a proton are added to enzyme reaction buffer, the rate of photoreactivation increases | Dunaliella salina | |
Na+ | - |
Dunaliella salina |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dunaliella salina | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by using glutathione-sepharose columns | Dunaliella salina |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(6-4) photoproduct (in DNA) = 2 pyrimidine residues (in DNA) | The overall repair reaction consists of two distinct steps, one of which is light-independent and the other one light-dependent. In the initial light-independent step, a 6-iminium ion is thought to be generated via proton transfer induced by two histidines highly conserved among the (6-4) photolyases.This intermediate spontaneously rearranges to form an oxetane intermediate by intramolecular nucleophilic attack. In the subsequent light-driven reaction, one electron is believed to be transferred from the fully reduced FAD cofactor (FADH-) to the oxetane intermediate thus forming a neutral FADH radical and an anionic oxetane radical, which spontaneously fractures. The excess electron is then back-transferred to the flavin radical restoring the fully reduced flavin cofactor and a pair of pyrimidine bases | Dunaliella salina |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | expression levels of (64) photolyase in 24 h dark-maintained algae increase rapidly after 8-h exposure to white light, and also increase after being treated with NaCl for 8 h | Dunaliella salina | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme shows activity with the GST tag | Dunaliella salina |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(6-4) photoproduct (in DNA) | - |
Dunaliella salina | pyrimidine residues (in DNA) | - |
? |
Synonyms | Comment | Organism |
---|---|---|
(6-4) photolyase | - |
Dunaliella salina |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
maximum activity occurs at pH 8.5 and is 2.4fold higher than that at pH 7.0 | Dunaliella salina |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9 | highest activity at | Dunaliella salina |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Dunaliella salina |