Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Xenopus laevis |
Protein Variants | Comment | Organism |
---|---|---|
H354A | almost complete loss of repair activity | Xenopus laevis |
H358A | almost complete loss of repair activity, suggesting that His354 and His358 are essential for catalytic activity | Xenopus laevis |
L355A | large decrease in the affinity to the (6-4) photoproduct substrate, suggesting a hydrophobic interaction with the (6-4)photoproduct | Xenopus laevis |
Q288A | repair activity is not reduced | Xenopus laevis |
W291A | some enzymatic activity is retained | Xenopus laevis |
W398A | some enzymatic activity is retained | Xenopus laevis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xenopus laevis | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(6-4) photoproduct (in DNA) = 2 pyrimidine residues (in DNA) | A mechanism is proposed in which the histidines residues His 354 and His 358 catalyze the formation of the four-membered ring intermediate in the repair process of this enzyme. When deuterium oxide is used as a solvent, the repair activity is decreased. The proton transfer shown by this isotope effect supports the proposed mechanism | Xenopus laevis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
T(6-4)T photoproduct (in DNA) | - |
Xenopus laevis | 2 thymidine residues (in DNA) | - |
? |
Synonyms | Comment | Organism |
---|---|---|
(6-4) photolyase | - |
Xenopus laevis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Xenopus laevis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9 | - |
Xenopus laevis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Xenopus laevis |