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Literature summary for 4.1.99.12 extracted from

  • Steinbacher, S.; Schiffmann, S.; Richter, G.; Huber, R.; Bacher, A.; Fischer, M.
    Structure of 3,4-dihydroxy-2-butanone 4-phosphate synthase from Methanococcus jannaschii in complex with divalent metal ions and the substrate ribulose 5-phosphate: implications for the catalytic mechanism (2003), J. Biol. Chem., 278, 42256-42265.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
E185X site-directed mutagenesis, inactive mutant Methanocaldococcus jannaschii
H147S site-directed mutagenesis, the mutant enzyme shows about 10% of the wild-type enzyme activity Methanocaldococcus jannaschii

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ binding structure, overview Methanocaldococcus jannaschii
Mg2+ binding structure, overview Methanocaldococcus jannaschii
additional information the enzyme depends on divalent metal ions, a dimetal center is involved in substrate binding Methanocaldococcus jannaschii
Zn2+ binding structure, overview Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-ribulose 5-phosphate Methanocaldococcus jannaschii step in the biosynthesis of riboflavin, vitamin B2, ribulose 5-phosphate is converted into 3,4-dihydroxy-2-butanone 4-phosphate while its C4 atom is released as formate in a sequence of metal-dependent reactions formate + L-3,4-dihydroxy-2-butanone-4-phosphate
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Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q60364
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Reaction

Reaction Comment Organism Reaction ID
D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate active site and reaction mechanism, structure-function relationship, structural coordination spheres are important, residues of the first coordination sphere involved in metal binding are indispensable for catalytic activity, Glu185 is essential for catalytic activity Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribulose 5-phosphate step in the biosynthesis of riboflavin, vitamin B2, ribulose 5-phosphate is converted into 3,4-dihydroxy-2-butanone 4-phosphate while its C4 atom is released as formate in a sequence of metal-dependent reactions Methanocaldococcus jannaschii formate + L-3,4-dihydroxy-2-butanone-4-phosphate
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D-ribulose 5-phosphate a dimetal center is involved in substrate binding, structure-function relationship, overview Methanocaldococcus jannaschii formate + L-3,4-dihydroxy-2-butanone-4-phosphate
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Subunits

Subunits Comment Organism
More structure-function relationship, overview Methanocaldococcus jannaschii

Synonyms

Synonyms Comment Organism
3,4-dihydroxy-2-butanone 4-phosphate synthase
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Methanocaldococcus jannaschii
DBPS
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Methanocaldococcus jannaschii