Protein Variants | Comment | Organism |
---|---|---|
E185X | site-directed mutagenesis, inactive mutant | Methanocaldococcus jannaschii |
H147S | site-directed mutagenesis, the mutant enzyme shows about 10% of the wild-type enzyme activity | Methanocaldococcus jannaschii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | binding structure, overview | Methanocaldococcus jannaschii | |
Mg2+ | binding structure, overview | Methanocaldococcus jannaschii | |
additional information | the enzyme depends on divalent metal ions, a dimetal center is involved in substrate binding | Methanocaldococcus jannaschii | |
Zn2+ | binding structure, overview | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose 5-phosphate | Methanocaldococcus jannaschii | step in the biosynthesis of riboflavin, vitamin B2, ribulose 5-phosphate is converted into 3,4-dihydroxy-2-butanone 4-phosphate while its C4 atom is released as formate in a sequence of metal-dependent reactions | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | Q60364 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate | active site and reaction mechanism, structure-function relationship, structural coordination spheres are important, residues of the first coordination sphere involved in metal binding are indispensable for catalytic activity, Glu185 is essential for catalytic activity | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose 5-phosphate | step in the biosynthesis of riboflavin, vitamin B2, ribulose 5-phosphate is converted into 3,4-dihydroxy-2-butanone 4-phosphate while its C4 atom is released as formate in a sequence of metal-dependent reactions | Methanocaldococcus jannaschii | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? | |
D-ribulose 5-phosphate | a dimetal center is involved in substrate binding, structure-function relationship, overview | Methanocaldococcus jannaschii | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structure-function relationship, overview | Methanocaldococcus jannaschii |
Synonyms | Comment | Organism |
---|---|---|
3,4-dihydroxy-2-butanone 4-phosphate synthase | - |
Methanocaldococcus jannaschii |
DBPS | - |
Methanocaldococcus jannaschii |