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Literature summary for 4.1.99.11 extracted from
- Evidence for benzylsuccinate synthase subtypes obtained by using stable isotope tools (2013), J. Bacteriol., 195, 4660-4667.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene bssA, phylogenetic analysis | Thauera aromatica |
| gene bssA, phylogenetic analysis | Azoarcus sp. |
| gene bssA, phylogenetic analysis | Desulfosarcina cetonica |
| gene bssA, phylogenetic analysis | Geobacter metallireducens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzylsuccinate | Thauera aromatica | - |
toluene + fumarate | - |
? |  |
| benzylsuccinate | Azoarcus sp. | - |
toluene + fumarate | - |
? | |
| benzylsuccinate | Desulfosarcina cetonica | - |
toluene + fumarate | - |
? | |
| benzylsuccinate | Geobacter metallireducens | - |
toluene + fumarate | - |
? | |
| benzylsuccinate | Geobacter metallireducens GS-15 / ATCC 53774 / DSM 7210 | - |
toluene + fumarate | - |
? | |
| benzylsuccinate | Desulfosarcina cetonica DSM 7267 | - |
toluene + fumarate | - |
? | |
| benzylsuccinate | Azoarcus sp. T | - |
toluene + fumarate | - |
? | |
| benzylsuccinate | Thauera aromatica K172 | - |
toluene + fumarate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Azoarcus sp. | Q8VPT7 | gene bssA | - |
| Azoarcus sp. T | Q8VPT7 | gene bssA | - |
| Desulfosarcina cetonica | A2TBP7 | gene bssA | - |
| Desulfosarcina cetonica DSM 7267 | A2TBP7 | gene bssA | - |
| Geobacter metallireducens | Q39VF1 | gene bssA | - |
| Geobacter metallireducens GS-15 / ATCC 53774 / DSM 7210 | Q39VF1 | gene bssA | - |
| Thauera aromatica | O87943 | gene bssA | - |
| Thauera aromatica K172 | O87943 | gene bssA | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.004 | - |
cell extract, pH 7.5, 30°C | Desulfosarcina cetonica |
| 0.015 | - |
cell extract, pH 7.5, 30°C | Geobacter metallireducens |
| 0.017 | - |
cell extract, pH 7.5, 30°C | Azoarcus sp. |
| 0.0195 | - |
cell extract, pH 7.5, 30°C | Thauera aromatica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzylsuccinate | - |
Thauera aromatica | toluene + fumarate | - |
? | |
| benzylsuccinate | - |
Azoarcus sp. | toluene + fumarate | - |
? | |
| benzylsuccinate | - |
Desulfosarcina cetonica | toluene + fumarate | - |
? | |
| benzylsuccinate | - |
Geobacter metallireducens | toluene + fumarate | - |
? | |
| benzylsuccinate | - |
Geobacter metallireducens GS-15 / ATCC 53774 / DSM 7210 | toluene + fumarate | - |
? | |
| benzylsuccinate | - |
Desulfosarcina cetonica DSM 7267 | toluene + fumarate | - |
? | |
| benzylsuccinate | - |
Azoarcus sp. T | toluene + fumarate | - |
? | |
| benzylsuccinate | - |
Thauera aromatica K172 | toluene + fumarate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BSSA | - |
Thauera aromatica |
| BSSA | - |
Azoarcus sp. |
| BSSA | - |
Desulfosarcina cetonica |
| BSSA | - |
Geobacter metallireducens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Thauera aromatica |
| 30 | - |
assay at | Azoarcus sp. |
| 30 | - |
assay at | Desulfosarcina cetonica |
| 30 | - |
assay at | Geobacter metallireducens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Thauera aromatica |
| 7.5 | - |
assay at | Azoarcus sp. |
| 7.5 | - |
assay at | Desulfosarcina cetonica |
| 7.5 | - |
assay at | Geobacter metallireducens |
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Release 2023.1 (January 2023)
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