Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Porphyromonas gingivalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
L-Trp | purified recombinant tryptophanase in 200 mM sodium phosphate buffer (pH 7.5), at 37°C | Porphyromonas gingivalis | |
0.2 | - |
L-Trp | purified recombinant tryptophanase in 200 mM potassium phosphate buffer (pH 7.5), at 37°C | Porphyromonas gingivalis | |
0.26 | - |
S-ethyl-L-cysteine | purified recombinant tryptophanase in 200 mM potassium phosphate buffer (pH 7.5), at 37°C | Porphyromonas gingivalis | |
2.04 | - |
S-methyl-L-cysteine | purified recombinant tryptophanase in 200 mM potassium phosphate buffer (pH 7.5), at 37°C | Porphyromonas gingivalis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | TnaA is not affected by K+ or Na+ | Porphyromonas gingivalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52500 | - |
calculated from amino acid sequence | Porphyromonas gingivalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Porphyromonas gingivalis | - |
- |
- |
Porphyromonas gingivalis W83 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
glutathione-Sepharose column chromatography | Porphyromonas gingivalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Trp + H2O | - |
Porphyromonas gingivalis | indole + pyruvate + NH4+ | - |
? | |
L-Trp + H2O | - |
Porphyromonas gingivalis W83 | indole + pyruvate + NH4+ | - |
? | |
additional information | tryptophanase does not degrade L-alanine, L-serine or L-cysteine | Porphyromonas gingivalis | ? | - |
? | |
additional information | tryptophanase does not degrade L-alanine, L-serine or L-cysteine | Porphyromonas gingivalis W83 | ? | - |
? | |
S-ethyl-L-cysteine + H2O | - |
Porphyromonas gingivalis | ? | - |
? | |
S-ethyl-L-cysteine + H2O | - |
Porphyromonas gingivalis W83 | ? | - |
? | |
S-methyl-L-cysteine + H2O | - |
Porphyromonas gingivalis | ? | - |
? | |
S-methyl-L-cysteine + H2O | - |
Porphyromonas gingivalis W83 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TnaA | - |
Porphyromonas gingivalis |
tryptophan indole-lyase | - |
Porphyromonas gingivalis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 70 | the enzyme is stable up to 40°C, but only 40% of the activity remains at 60°C, the enzyme is completely inactivated at temperatures above 70°C | Porphyromonas gingivalis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
L-Trp | purified recombinant tryptophanase in 200 mM sodium phosphate buffer (pH 7.5), at 37°C | Porphyromonas gingivalis | |
0.5 | - |
S-methyl-L-cysteine | purified recombinant tryptophanase in 200 mM potassium phosphate buffer (pH 7.5), at 37°C | Porphyromonas gingivalis | |
1.37 | - |
L-Trp | purified recombinant tryptophanase in 200 mM potassium phosphate buffer (pH 7.5), at 37°C | Porphyromonas gingivalis | |
1.87 | - |
S-ethyl-L-cysteine | purified recombinant tryptophanase in 200 mM potassium phosphate buffer (pH 7.5), at 37°C | Porphyromonas gingivalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Porphyromonas gingivalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Porphyromonas gingivalis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
S-methyl-L-cysteine | purified recombinant tryptophanase in 200 mM potassium phosphate buffer (pH 7.5), at 37°C | Porphyromonas gingivalis | |
6.87 | - |
L-Trp | purified recombinant tryptophanase in 200 mM potassium phosphate buffer (pH 7.5), at 37°C | Porphyromonas gingivalis | |
7.15 | - |
S-ethyl-L-cysteine | purified recombinant tryptophanase in 200 mM potassium phosphate buffer (pH 7.5), at 37°C | Porphyromonas gingivalis |