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Literature summary for 4.1.99.1 extracted from
- The tryptophanase from Proteus rettgeri, improved purification and properties of crystalline holotryptophanase (1975), Biochim. Biophys. Acta, 391, 494-503.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Providencia rettgeri |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| guanidine hydrochloride | - |
Providencia rettgeri |  |
| SDS | - |
Providencia rettgeri | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.26 | - |
L-Trp | - |
Providencia rettgeri | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
4 * 55000, SDS-PAGE | Providencia rettgeri |
| 222000 | - |
ultracentrifugation and sedimentation measurement | Providencia rettgeri |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Providencia rettgeri | inducible enzyme | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Providencia rettgeri | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Providencia rettgeri |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 9.01 | - |
- |
Providencia rettgeri |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 5°C, stored as a suspension in 0.1 M potassium phosphate buffer, pH 8.0, containing 20% v/v glycerol, 0.1 mM pyridoxal 5'-phosphate, 10 mM mercaptoethanol and 60% saturated ammonium sulfate, stable for more than 5 months | Providencia rettgeri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Trp + H2O | - |
Providencia rettgeri | indole + pyruvate + NH4+ | - |
? | |
| additional information | inducible enzyme | Providencia rettgeri | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 55000, SDS-PAGE | Providencia rettgeri |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | contains 4 mol of pyridoxal 5'-phosphate per mol of enzyme | Providencia rettgeri | |
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Release 2023.1 (January 2023)
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