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Literature summary for 4.1.3.B3 extracted from
- Assimilation of aromatic compounds by Comamonas testosteroni: characterization and spreadability of protocatechuate 4,5-cleavage pathway in bacteria (2013), Appl. Microbiol. Biotechnol., 97, 6031-6041.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene pmdF, orf CtCNB1_2744, DNA and amino acid sequence determination and analysis, genetic organization, phylogenetic analysis, expression in Escherichia coli | Comamonas testosteroni |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | gene disruption and complementation of genes pmdE, pmdF, and pmdU in Comamonas testosteroni wild-type and mutants, overview | Comamonas testosteroni |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-carboxy-4-hydroxy-2-oxoadipate | Comamonas testosteroni | - |
oxaloacetate + pyruvate | - |
? |  |
| 4-carboxy-4-hydroxy-2-oxoadipate | Comamonas testosteroni CNB-1 | - |
oxaloacetate + pyruvate | - |
? | |
| additional information | Comamonas testosteroni | the bifunctional enzyme PmdF shows obvious aldolase activity when 4-carboxy-4-hydroxy-2-oxoadipate is used as substrate and obvious oxalacetate decarboxylase activity when oxaloacetate is used as substrate | ? | - |
? | |
| additional information | Comamonas testosteroni CNB-1 | the bifunctional enzyme PmdF shows obvious aldolase activity when 4-carboxy-4-hydroxy-2-oxoadipate is used as substrate and obvious oxalacetate decarboxylase activity when oxaloacetate is used as substrate | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Comamonas testosteroni | Q93PS8 | gene pmdF | - |
| Comamonas testosteroni CNB-1 | Q93PS8 | gene pmdF | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli | Comamonas testosteroni |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | strain CNB-1 grows on phenol, gentisate, vanillate, 3-hydroxybenzoate, and 4-hydroxybenzoate as carbon and energy sources | Comamonas testosteroni | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-carboxy-4-hydroxy-2-oxoadipate | - |
Comamonas testosteroni | oxaloacetate + pyruvate | - |
? | |
| 4-carboxy-4-hydroxy-2-oxoadipate | - |
Comamonas testosteroni CNB-1 | oxaloacetate + pyruvate | - |
? | |
| additional information | the bifunctional enzyme PmdF shows obvious aldolase activity when 4-carboxy-4-hydroxy-2-oxoadipate is used as substrate and obvious oxalacetate decarboxylase activity when oxaloacetate is used as substrate | Comamonas testosteroni | ? | - |
? | |
| additional information | the bifunctional enzyme PmdF shows obvious aldolase activity when 4-carboxy-4-hydroxy-2-oxoadipate is used as substrate and obvious oxalacetate decarboxylase activity when oxaloacetate is used as substrate | Comamonas testosteroni CNB-1 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 4-carboxy-4-hydroxy-2-oxoadipate aldolase/oxaloacetate decarboxylase | - |
Comamonas testosteroni |
| CHA aldolase | - |
Comamonas testosteroni |
| PmdF | - |
Comamonas testosteroni |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at room temperature | Comamonas testosteroni |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the protocatechuate 4,5-cleavage pathway is mainly distributed in alpha- and beta-proteobacteria, gene clusters of protocatechuate 4,5-cleavage pathway in strain CNB-1 and other bacterial strains, genotyping and phylogenetic analysis, overview. Two types of genetic organization/cluster are recognized for PCA 4,5-cleavage pathway: The Sphingobium-type gene cluster constitutes several transcriptional units. The Comamonas-Pseudomonas type constitutes only one transcriptional unit | Comamonas testosteroni |
| metabolism | the enzyme catalyzes the last step of the protocatechuate 4,5-cleavage pathway. Vanillate, 3-hydroxybenzoate, and 4-hydroxybenzoate are degraded via protocatechuate 4,5-cleavage pathway | Comamonas testosteroni |
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