BRENDA - Enzyme Database show
show all sequences of 4.1.3.6

Citrate in urine determined with a new citrate lyase method

Petrarulo, M.; Facchini, P.; Cerelli, E.; Marangella, M.; Linari, F.; Clin. Chem. 41, 1518-1521 (1995)

Data extracted from this reference:

Application
Application
Commentary
Organism
analysis
determination of citrate in urine with a new citrate lyase method
Klebsiella pneumoniae
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Klebsiella pneumoniae
-
-
-
Storage Stability
Storage Stability
Organism
-20°C, stable for at least 6 months
Klebsiella pneumoniae
4°C, stable for at least 1 month
Klebsiella pneumoniae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
citrate
-
33372
Klebsiella pneumoniae
acetate + oxaloacetate
-
33372
Klebsiella pneumoniae
?
Application (protein specific)
Application
Commentary
Organism
analysis
determination of citrate in urine with a new citrate lyase method
Klebsiella pneumoniae
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, stable for at least 6 months
Klebsiella pneumoniae
4°C, stable for at least 1 month
Klebsiella pneumoniae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
citrate
-
33372
Klebsiella pneumoniae
acetate + oxaloacetate
-
33372
Klebsiella pneumoniae
?
Other publictions for EC 4.1.3.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747969
Ouattara
Lactic acid bacteria involved ...
Lactobacillus casei, Lactobacillus casei T10G5, Lactobacillus plantarum, Lactobacillus plantarum T10AG26, Lactobacillus plantarum T11G3, Lactobacillus plantarum T8N10, Leuconostoc mesenteroides, Leuconostoc mesenteroides T8AB6
Int. J. Food Microbiol.
256
11-19
2017
-
-
-
-
-
-
-
-
-
-
-
8
-
8
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
3
-
-
3
-
-
747566
Chung
Induced self-assembly of plat ...
Klebsiella pneumoniae
Chemistry
20
13016-13027
2014
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726740
Mortera
Ca2+-citrate uptake and metabo ...
Lactobacillus casei, Lactobacillus casei ATCC 334
Appl. Environ. Microbiol.
79
4603-4612
2013
-
-
-
-
-
-
-
-
-
1
-
2
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
694565
Srivastava
Genetic correlates of olanzapi ...
Homo sapiens
Pharmacogenomics
9
1055-1068
2008
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
674281
Hu
Citric acid cycle in the hyper ...
Pyrobaculum islandicum
J. Bacteriol.
188
4350-4355
2006
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
662015
Martin
Acid-inducible transcription o ...
Lactococcus lactis
J. Bacteriol.
186
5649-5660
2004
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
492186
Schneider
Identification of a gene clust ...
Klebsiella pneumoniae
J. Bacteriol.
184
2439-2446
2002
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
649579
Morikawa
Molecular cloning of novel mou ...
Homo sapiens, Mus musculus
Biochem. Biophys. Res. Commun.
289
1282-1286
2001
-
-
2
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
8
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
8
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
653146
de Figueroa
Influence of temperature on fl ...
Lactobacillus rhamnosus
Microbiol. Res.
155
257-262
2001
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
441599
Schneider
Biosynthesis of the prosthetic ...
Escherichia coli, Klebsiella pneumoniae
Biochemistry
39
9438-9450
2000
-
-
1
-
-
-
-
-
-
-
8
2
-
2
-
-
2
-
-
-
1
-
2
1
1
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
8
2
-
-
-
2
-
-
1
-
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
33346
Bekal
Purification of Leuconostoc me ...
Leuconostoc mesenteroides
J. Bacteriol.
180
647-654
1998
-
-
1
-
-
-
-
-
-
-
3
-
-
1
-
-
1
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
1
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
33349
Bekal
-
Citrate lyases of lactic acid ...
Clostridium sphenoides, Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae, Lactococcus lactis, Lactococcus lactis subsp. diacetylactis, Lactococcus lactis subsp. lactis, Leuconostoc mesenteroides, Rubrivivax gelatinosus
Lait
78
3-10
1998
-
-
-
-
-
-
-
-
-
-
17
-
-
9
-
3
-
-
-
-
-
-
9
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
17
-
-
-
3
-
-
-
-
-
9
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
33367
Matsumoto
-
Simultaneous quantitation of c ...
Klebsiella pneumoniae
Anal. Chim. Acta
321
157-164
1996
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
33368
David
-
Quantification of citrate lyas ...
Lactococcus lactis
Appl. Microbiol. Biotechnol.
44
68-74
1995
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
33372
Petrarulo
Citrate in urine determined wi ...
Klebsiella pneumoniae
Clin. Chem.
41
1518-1521
1995
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
33370
Bott
Klebsiella pneumoniae genes fo ...
Klebsiella pneumoniae
Mol. Microbiol.
14
347-356
1994
-
-
1
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
698
Quentmeier
Characterization of citrate ly ...
Clostridium sporosphaeroides
Arch. Microbiol.
141
85-90
1985
1
-
-
-
-
-
-
1
-
5
4
-
-
1
-
-
1
-
-
-
2
1
1
1
1
-
2
-
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
5
4
-
-
-
-
1
-
-
2
1
1
1
1
-
2
-
1
1
-
-
-
-
-
-
-
-
33363
Subramanian
-
Bacterial citrate lyase ...
Aerobacter indologenes, Clostridium indolis, Clostridium rectum, Clostridium sphenoides, Clostridium sporogenes, Clostridium sporosphaeroides, Clostridium subterminale, Clostridium symbiosum, Enterobacter cloacae, Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae, Lactococcus lactis subsp. lactis, Leuconostoc citrovorum, no activity in Chlorobium thiosulfatophilum, no activity in Rhodospirillum rubrum, Providencia rettgeri, Rhodopseudomonas palustris, Rubrivivax gelatinosus, Salmonella enterica subsp. enterica serovar Typhimurium
J. Biosci.
6
379-401
1984
1
-
-
-
-
3
3
-
-
21
6
40
-
20
-
-
-
-
-
-
6
6
62
6
-
-
-
-
6
-
-
-
-
-
-
1
-
-
-
-
-
3
-
3
-
-
-
21
6
40
-
-
-
-
-
-
6
6
62
6
-
-
-
-
6
-
-
-
-
-
-
-
-
-
33364
Nilekani
Purification and properties of ...
Escherichia coli
Biochemistry
22
4657-4663
1983
1
-
-
-
-
-
-
-
-
1
2
-
-
1
-
1
1
-
-
-
1
1
1
1
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
1
1
-
-
1
1
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
33362
Antranikian
Purification of L-glutamate-de ...
Clostridium sphenoides, Lactococcus lactis subsp. lactis, Rubrivivax gelatinosus
Eur. J. Biochem.
126
35-42
1982
1
-
-
-
-
1
-
1
-
5
1
1
-
3
-
-
1
-
-
-
3
3
4
-
-
-
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
1
-
5
1
1
-
-
-
1
-
-
3
3
4
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
33361
Sivaraman
Cooperative binding of mangane ...
Klebsiella pneumoniae
FEBS Lett.
105
267-270
1979
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
33360
Giffhorn
Crystallization and subunit co ...
Rubrivivax gelatinosus
FEBS Lett.
96
175-178
1978
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
33357
Hiremath
Purification and properties of ...
Enterococcus faecalis
Biochem. Biophys. Res. Commun.
72
1122-1128
1976
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
693
Kummel
Citrate lyase from Streptococc ...
Klebsiella pneumoniae, Lactococcus lactis subsp. lactis, Leuconostoc citrovorum, Rubrivivax gelatinosus
Arch. Microbiol.
102
111-116
1975
-
-
-
-
-
-
4
-
-
-
-
-
-
4
-
-
1
-
-
-
3
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
1
-
-
3
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
33356
Carpenter
On the molecular weights of th ...
Klebsiella pneumoniae
J. Biol. Chem.
250
3254-3260
1975
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
33358
Srere
The enzymology of the formatio ...
Klebsiella pneumoniae, Lactococcus lactis subsp. lactis
Adv. Enzymol. Relat. Areas Mol. Biol.
43
57-101
1975
1
-
-
-
-
-
2
-
-
-
-
1
-
2
-
1
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
33369
Dimroth
Isolation of subunits of citra ...
Klebsiella pneumoniae
Proc. Natl. Acad. Sci. USA
72
3458-3462
1975
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
33353
Singh
Nonidentical subunits of citra ...
Klebsiella pneumoniae
Biochem. Biophys. Res. Commun.
59
1211-1218
1974
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
33354
Beuscher
Citrate lyase from Rhodopseudo ...
Rubrivivax gelatinosus
Arch. Microbiol.
100
307-328
1974
-
-
-
-
-
-
-
2
-
-
4
-
-
1
-
-
1
-
-
-
1
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
4
-
-
-
-
1
-
-
1
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
33351
Srere
Citrate lyase: a pantothenate- ...
Klebsiella pneumoniae
Proc. Natl. Acad. Sci. USA
69
1201-1202
1972
1
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
33352
Spector
-
Citrate cleavage and related e ...
Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae, Lactococcus lactis subsp. lactis
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
7
357-389
1972
-
-
-
-
-
-
4
2
-
14
1
-
-
4
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
2
-
14
1
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
33350
Bowen
Sub-units of citrate oxaloacet ...
Klebsiella pneumoniae
Eur. J. Biochem.
23
262-266
1971
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-