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Literature summary for 4.1.3.6 extracted from
- Purification and properties of citrate lyase from Escherichia coli (1983), Biochemistry, 22, 4657-4663.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| pantothenate | contains 3.5 mol pantothenate per mol of enzyme in the acyl-carrier-protein gamma-subunit | Escherichia coli |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | optimal concentration: 10 mM | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
alpha6beta6gamma, 6 * 32000, acyllyase beta-subunit, + 6 * 54000, acyltransferase alpha-subunit + 1 * 85000,acyl-carrier-protein gamma-subunit, SDS-PAGE | Escherichia coli |
| 54000 | - |
alpha6beta6gamma, 6 * 32000, acyllyase beta-subunit, + 6 * 54000, acyltransferase alpha-subunit + 1 * 85000,acyl-carrier-protein gamma-subunit, SDS-PAGE | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| side-chain modification | acetyl-enzyme | Escherichia coli |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.12 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C or -20°C, stable for at least 2-3 days | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| citrate | - |
Escherichia coli | acetate + oxaloacetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| oligomer | alpha6beta6gamma, 6 * 32000, acyllyase beta-subunit, + 6 * 54000, acyltransferase alpha-subunit + 1 * 85000,acyl-carrier-protein gamma-subunit, SDS-PAGE | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Escherichia coli |
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