Protein Variants | Comment | Organism |
---|---|---|
C327A | the mutation leads to decreased activity (6%) compared to the wild type enzyme and results in a complete loss of product selectivity | Streptomyces hygroscopicus |
C327A | the Hyg5 mutant shows 6% decreased activity compared to the wild-type, and results in a complete loss of product selectivity. The FkbO product (4R,5R)-4,5-dihydroxycyclohexa-1(6),2-diene-1-carboxylate as well as the XanB2 product 4-hydroxybenzoate are produced | Streptomyces hygroscopicus |
C327A/G240A | the Hyg5 double mutant shows the same behavior as the single mutants, but along with a more dramatic loss of activity | Streptomyces hygroscopicus |
G240A | the mutation leads to decreased activity (55%) compared to the wild type enzyme and results in a complete loss of product selectivity | Streptomyces hygroscopicus |
G240A | the Hyg5 mutant shows 55% decreased activity compared to the wild-type, and results in a complete loss of product selectivity. The FkbO product (4R,5R)-4,5-dihydroxycyclohexa-1(6),2-diene-1-carboxylate as well as the XanB2 product 4-hydroxybenzoate are produced | Streptomyces hygroscopicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-(2-carboxyethyl) benzoate | - |
Streptomyces hygroscopicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chorismate | Streptomyces hygroscopicus | - |
3-hydroxybenzoate + pyruvate | - |
? | |
chorismate | Streptomyces hygroscopicus | - |
3-hydroxybenzoate + pyruvate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces hygroscopicus | O30478 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
chorismate = 3-hydroxybenzoate + pyruvate | a hydrolysis-elimination mechanism for CH-Hyg5 is observed. No external oxygen is incorporated into the Hyg5 products indicated an intramolecular mechanism for the Hyg5 reaction. A pericyclic mechanism, as described for chorismate-converting enzymes producing the 2- and 4-regioisomeric hydroxybenzoates, can also be ruled out due to the position of the hydroxyl group in 3-hydroxybenzoate. Arene oxide reaction mechansim analysis, overview | Streptomyces hygroscopicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chorismate | - |
Streptomyces hygroscopicus | 3-hydroxybenzoate + pyruvate | - |
? | |
chorismate | - |
Streptomyces hygroscopicus | 3-hydroxybenzoate + pyruvate | - |
ir | |
additional information | no activity with 3,4-trans-dihydroxy-cyclohexa-1,5-diene-1-carboxylate and enoyl benzoate by enzyme Hyg5 | Streptomyces hygroscopicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-hydroxybenzoate synthase | - |
Streptomyces hygroscopicus |
CH-Hyg5 | - |
Streptomyces hygroscopicus |
chorismatase | - |
Streptomyces hygroscopicus |
chorismatase/3-hydroxybenzoate synthase | - |
Streptomyces hygroscopicus |
hyg5 | - |
Streptomyces hygroscopicus |
General Information | Comment | Organism |
---|---|---|
evolution | three subfamilies of chorismatases are described that convert chorismate into different (dihydro-)benzoate derivatives (CH-FkbO, CH-Hyg5, and CH-XanB2). The CH-FkbO and CH-Hyg5 subfamilies share the same protein fold, but employ fundamentally different reaction mechanisms, comparisons of the reaction mechanism of CH-FkbO and CH-Hyg5, and structure-function analysis, overview | Streptomyces hygroscopicus |
additional information | involvement of residue C327 in directing the selectivity of product formation is underlined by the crystal structure of the mutant Hyg5 C327S with the product 3-hydroxybenzoate | Streptomyces hygroscopicus |