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Literature summary for 4.1.3.44 extracted from

  • Grell, T.; Young, A.; Drennan, C.; Bandarian, V.
    Biochemical and structural characterization of a Schiff Base in the radical-mediated biosynthesis of 4-demethylwyosine by TYW1 (2018), J. Am. Chem. Soc., 140, 6842-6852 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 100 mM KH2PO4/Na2HPO4 pH 6.2, 10% (w/v) PEG 3000 Methanocaldococcus jannaschii

Protein Variants

Protein Variants Comment Organism
C195S the mutant shows reduced activity compared tot he wild type enzyme Methanocaldococcus jannaschii
K41A inactive Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N1-methylguanine37 in tRNAPhe + pyruvate + S-adenosyl-L-methionine Methanocaldococcus jannaschii
-
4-demethylwyosine37 in tRNAPhe + L-methionine + 5'-deoxyadenosine + CO2 + H2O
-
ir
N1-methylguanine37 in tRNAPhe + pyruvate + S-adenosyl-L-methionine Methanocaldococcus jannaschii DSM 2661
-
4-demethylwyosine37 in tRNAPhe + L-methionine + 5'-deoxyadenosine + CO2 + H2O
-
ir

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q57705
-
-
Methanocaldococcus jannaschii DSM 2661 Q57705
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, butyl Sepharose column chromatography, and HiTrap chelating column chromatography Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N1-methylguanine37 in tRNAPhe + pyruvate + S-adenosyl-L-methionine
-
Methanocaldococcus jannaschii 4-demethylwyosine37 in tRNAPhe + L-methionine + 5'-deoxyadenosine + CO2 + H2O
-
ir
N1-methylguanine37 in tRNAPhe + pyruvate + S-adenosyl-L-methionine
-
Methanocaldococcus jannaschii DSM 2661 4-demethylwyosine37 in tRNAPhe + L-methionine + 5'-deoxyadenosine + CO2 + H2O
-
ir

Synonyms

Synonyms Comment Organism
TYW1
-
Methanocaldococcus jannaschii

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
Methanocaldococcus jannaschii
[4Fe-4S]-center
-
Methanocaldococcus jannaschii