BRENDA - Enzyme Database
show all sequences of 4.1.3.43

Rational design of stereoselectivity in the class II pyruvate aldolase BphI

Baker, P.; Seah, S.Y.K.; J. Am. Chem. Soc. 134, 507-513 (2012)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
variants of BphI expressed in recombinant Escherichia coli BL21, lambdaDE3 cells
Paraburkholderia xenovorans
Engineering
Protein Variants
Commentary
Organism
L87N
mutant created by site-specific mutagenesis
Paraburkholderia xenovorans
L87N/Y290F
exhibits stereoselectivity opposite to that of the wild type and alkyl chain length of the aldehyde does not affect stereochemical control
Paraburkholderia xenovorans
L87W
mutant created by site-specific mutagenesis
Paraburkholderia xenovorans
L87W/Y290F
exhibits stereoselectivity opposite to that of the wild type and alkyl chain length of the aldehyde does not affect stereochemical control
Paraburkholderia xenovorans
additional information
mutants created by site-specific mutagenesis
Paraburkholderia xenovorans
Y290F
mutant is able to completely degrade racemic mixtures of 4-hydroxy-2-oxoacids i.e. is not stereospecific
Paraburkholderia xenovorans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.013
-
(4R)-4-hydroxy-2-oxopentanoate
Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.013
-
(4S)-4-hydroxy-2-oxopentanoate
Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.089
-
(4S)-4-hydroxy-2-oxopentanoate
wild-type BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.21
-
oxaloacetate
L87N variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.435
-
(4R)-4-hydroxy-2-oxopentanoate
L87W/Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.757
-
(4R)-4-hydroxy-2-oxopentanoate
L87N/Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.84
-
oxaloacetate
L87N/Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.92
-
oxaloacetate
Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
6.2
-
oxaloacetate
L87W/Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
7.4
-
oxaloacetate
wild-type BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
13
-
pyruvate
wild-type BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
20
-
pyruvate
Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
24.4
-
propionaldehyde
L87W/Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
30.2
-
pyruvate
L87N/Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
38.2
-
pyruvate
L87W/Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
54.9
-
propionaldehyde
L87N/Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
64.2
-
acetaldehyde
wild-type BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
70.7
-
acetaldehyde
L87N/Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
80.5
-
pentaldehyde
Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
81
-
acetaldehyde
Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
81.2
-
propionaldehyde
Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
86.3
-
acetaldehyde
L87W/Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
104
-
Butyraldehyde
Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
136
-
propionaldehyde
wild-type BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
Organism
Organism
UniProt
Commentary
Textmining
Paraburkholderia xenovorans
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(4R)-4-hydroxy-2-oxohexanoate
reaction catalyzed by BphI variants Y290F, L87N/Y290F and L87W/Y290F
722458
Paraburkholderia xenovorans
pyruvate + propionaldehyde
-
-
-
?
(4R)-4-hydroxy-2-oxopentanoate
-
722458
Paraburkholderia xenovorans
pyruvate + acetaldehyde
-
-
-
?
(4S)-4-hydroxy-2-oxohexanoate
double variants L87N/Y290F and L87W/Y290F inactive toward (4S)-4-hydroxy-2-oxohexanoate
722458
Paraburkholderia xenovorans
pyruvate + propionaldehyde
-
-
-
?
(4S)-4-hydroxy-2-oxopentanoate
-
722458
Paraburkholderia xenovorans
pyruvate + acetaldehyde
-
-
-
?
Oxaloacetate
oxaloacetate decarboxylation is a secondary reaction of pyruvate aldolases
722458
Paraburkholderia xenovorans
CO2 + pyruvate
-
-
-
?
pyruvate + acetaldehyde
-
722458
Paraburkholderia xenovorans
(4R)-4-hydroxy-2-oxopentanoate
-
-
-
?
pyruvate + acetaldehyde
-
722458
Paraburkholderia xenovorans
(4S)-4-hydroxy-2-oxopentanoate
-
-
-
?
pyruvate + butyraldehyde
-
722458
Paraburkholderia xenovorans
?
-
-
-
?
pyruvate + pentaldehyde
-
722458
Paraburkholderia xenovorans
?
-
-
-
?
pyruvate + propionaldehyde
-
722458
Paraburkholderia xenovorans
4-hydroxy-2-oxohexanoate
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
BphI
-
Paraburkholderia xenovorans
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.043
-
(4R)-4-hydroxy-2-oxopentanoate
L87W/Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.05
-
oxaloacetate
L87N variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.08
-
oxaloacetate
L87N/Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.098
-
(4R)-4-hydroxy-2-oxopentanoate
L87N/Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.1
-
propionaldehyde
L87N/Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.11
-
pyruvate
L87N/Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.131
-
(4R)-4-hydroxy-2-oxopentanoate
Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.132
-
(4S)-4-hydroxy-2-oxopentanoate
Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.14
-
pentaldehyde
Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.14
-
propionaldehyde
L87W/Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.16
-
Butyraldehyde
Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.16
-
propionaldehyde
Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.16
-
pyruvate
L87W/Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.19
-
pyruvate
Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.2
-
acetaldehyde
Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.27
-
oxaloacetate
Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.3
-
acetaldehyde
L87N/Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.4
-
oxaloacetate
L87W/Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.59
-
acetaldehyde
L87W/Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.86
-
acetaldehyde
wild-type BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
1.2
-
pyruvate
wild-type BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
1.79
-
propionaldehyde
wild-type BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
4
-
(4S)-4-hydroxy-2-oxopentanoate
wild-type BphI, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
16.3
-
oxaloacetate
wild-type BphI, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
Cloned(Commentary) (protein specific)
Commentary
Organism
variants of BphI expressed in recombinant Escherichia coli BL21, lambdaDE3 cells
Paraburkholderia xenovorans
Engineering (protein specific)
Protein Variants
Commentary
Organism
L87N
mutant created by site-specific mutagenesis
Paraburkholderia xenovorans
L87N/Y290F
exhibits stereoselectivity opposite to that of the wild type and alkyl chain length of the aldehyde does not affect stereochemical control
Paraburkholderia xenovorans
L87W
mutant created by site-specific mutagenesis
Paraburkholderia xenovorans
L87W/Y290F
exhibits stereoselectivity opposite to that of the wild type and alkyl chain length of the aldehyde does not affect stereochemical control
Paraburkholderia xenovorans
additional information
mutants created by site-specific mutagenesis
Paraburkholderia xenovorans
Y290F
mutant is able to completely degrade racemic mixtures of 4-hydroxy-2-oxoacids i.e. is not stereospecific
Paraburkholderia xenovorans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.013
-
(4R)-4-hydroxy-2-oxopentanoate
Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.013
-
(4S)-4-hydroxy-2-oxopentanoate
Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.089
-
(4S)-4-hydroxy-2-oxopentanoate
wild-type BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.21
-
oxaloacetate
L87N variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.435
-
(4R)-4-hydroxy-2-oxopentanoate
L87W/Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.757
-
(4R)-4-hydroxy-2-oxopentanoate
L87N/Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.84
-
oxaloacetate
L87N/Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.92
-
oxaloacetate
Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
6.2
-
oxaloacetate
L87W/Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
7.4
-
oxaloacetate
wild-type BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
13
-
pyruvate
wild-type BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
20
-
pyruvate
Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
24.4
-
propionaldehyde
L87W/Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
30.2
-
pyruvate
L87N/Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
38.2
-
pyruvate
L87W/Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
54.9
-
propionaldehyde
L87N/Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
64.2
-
acetaldehyde
wild-type BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
70.7
-
acetaldehyde
L87N/Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
80.5
-
pentaldehyde
Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
81
-
acetaldehyde
Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
81.2
-
propionaldehyde
Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
86.3
-
acetaldehyde
L87W/Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
104
-
Butyraldehyde
Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
136
-
propionaldehyde
wild-type BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(4R)-4-hydroxy-2-oxohexanoate
reaction catalyzed by BphI variants Y290F, L87N/Y290F and L87W/Y290F
722458
Paraburkholderia xenovorans
pyruvate + propionaldehyde
-
-
-
?
(4R)-4-hydroxy-2-oxopentanoate
-
722458
Paraburkholderia xenovorans
pyruvate + acetaldehyde
-
-
-
?
(4S)-4-hydroxy-2-oxohexanoate
double variants L87N/Y290F and L87W/Y290F inactive toward (4S)-4-hydroxy-2-oxohexanoate
722458
Paraburkholderia xenovorans
pyruvate + propionaldehyde
-
-
-
?
(4S)-4-hydroxy-2-oxopentanoate
-
722458
Paraburkholderia xenovorans
pyruvate + acetaldehyde
-
-
-
?
Oxaloacetate
oxaloacetate decarboxylation is a secondary reaction of pyruvate aldolases
722458
Paraburkholderia xenovorans
CO2 + pyruvate
-
-
-
?
pyruvate + acetaldehyde
-
722458
Paraburkholderia xenovorans
(4R)-4-hydroxy-2-oxopentanoate
-
-
-
?
pyruvate + acetaldehyde
-
722458
Paraburkholderia xenovorans
(4S)-4-hydroxy-2-oxopentanoate
-
-
-
?
pyruvate + butyraldehyde
-
722458
Paraburkholderia xenovorans
?
-
-
-
?
pyruvate + pentaldehyde
-
722458
Paraburkholderia xenovorans
?
-
-
-
?
pyruvate + propionaldehyde
-
722458
Paraburkholderia xenovorans
4-hydroxy-2-oxohexanoate
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.043
-
(4R)-4-hydroxy-2-oxopentanoate
L87W/Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.05
-
oxaloacetate
L87N variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.08
-
oxaloacetate
L87N/Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.098
-
(4R)-4-hydroxy-2-oxopentanoate
L87N/Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.1
-
propionaldehyde
L87N/Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.11
-
pyruvate
L87N/Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.131
-
(4R)-4-hydroxy-2-oxopentanoate
Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.132
-
(4S)-4-hydroxy-2-oxopentanoate
Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.14
-
pentaldehyde
Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.14
-
propionaldehyde
L87W/Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.16
-
Butyraldehyde
Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.16
-
propionaldehyde
Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.16
-
pyruvate
L87W/Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.19
-
pyruvate
Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.2
-
acetaldehyde
Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.27
-
oxaloacetate
Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.3
-
acetaldehyde
L87N/Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.4
-
oxaloacetate
L87W/Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.59
-
acetaldehyde
L87W/Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.86
-
acetaldehyde
wild-type BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
1.2
-
pyruvate
wild-type BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
1.79
-
propionaldehyde
wild-type BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
4
-
(4S)-4-hydroxy-2-oxopentanoate
wild-type BphI, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
16.3
-
oxaloacetate
wild-type BphI, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.00004
-
pentaldehyde
L87N/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.000051
-
pentaldehyde
L87W/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.00016
-
Butyraldehyde
L87N/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.00031
-
Butyraldehyde
L87W/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.00047
-
pentaldehyde
wild-type BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.0015
-
Butyraldehyde
Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.0017
-
pentaldehyde
Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.0018
-
propionaldehyde
L87N/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.002
-
propionaldehyde
Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.00219
-
Butyraldehyde
wild-type BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.0025
-
acetaldehyde
Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.0036
-
pyruvate
L87N/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.0043
-
acetaldehyde
L87N/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.0043
-
pyruvate
L87W/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.0057
-
propionaldehyde
L87W/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.0058
-
oxaloacetate
L87W variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.0069
-
acetaldehyde
L87W/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.0084
-
pyruvate
Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.0132
-
propionaldehyde
wild-type BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.0134
-
acetaldehyde
wild-type BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.064
-
oxaloacetate
L87W/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.0915
-
pyruvate
wild-type BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.098
-
oxaloacetate
L87N/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.1
-
(4R)-4-hydroxy-2-oxopentanoate
L87W/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.13
-
(4R)-4-hydroxy-2-oxopentanoate
L87N/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.254
-
oxaloacetate
L87N variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.53
-
oxaloacetate
Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
2.2
-
oxaloacetate
wild-type BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
10.4
-
(4R)-4-hydroxy-2-oxopentanoate
Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
12.6
-
(4S)-4-hydroxy-2-oxopentanoate
Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
44.7
-
(4S)-4-hydroxy-2-oxopentanoate
wild-type BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.00004
-
pentaldehyde
L87N/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.000051
-
pentaldehyde
L87W/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.00016
-
Butyraldehyde
L87N/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.00031
-
Butyraldehyde
L87W/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.00047
-
pentaldehyde
wild-type BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.0015
-
Butyraldehyde
Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.0017
-
pentaldehyde
Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.0018
-
propionaldehyde
L87N/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.002
-
propionaldehyde
Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.00219
-
Butyraldehyde
wild-type BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.0025
-
acetaldehyde
Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.0036
-
pyruvate
L87N/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.0043
-
acetaldehyde
L87N/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.0043
-
pyruvate
L87W/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.0057
-
propionaldehyde
L87W/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.0058
-
oxaloacetate
L87W variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.0069
-
acetaldehyde
L87W/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.0084
-
pyruvate
Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.0132
-
propionaldehyde
wild-type BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.0134
-
acetaldehyde
wild-type BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.064
-
oxaloacetate
L87W/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.0915
-
pyruvate
wild-type BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.098
-
oxaloacetate
L87N/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.1
-
(4R)-4-hydroxy-2-oxopentanoate
L87W/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.13
-
(4R)-4-hydroxy-2-oxopentanoate
L87N/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.254
-
oxaloacetate
L87N variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
0.53
-
oxaloacetate
Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
2.2
-
oxaloacetate
wild-type BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
10.4
-
(4R)-4-hydroxy-2-oxopentanoate
Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
12.6
-
(4S)-4-hydroxy-2-oxopentanoate
Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
44.7
-
(4S)-4-hydroxy-2-oxopentanoate
wild-type BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
Paraburkholderia xenovorans
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721659
Baker
Protein-protein interactions a ...
Thermus thermophilus
Biochemistry
51
1942-1952
2012
-
-
1
-
1
-
-
16
-
-
7
-
-
4
-
-
1
-
-
-
-
-
4
1
1
-
-
1
15
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
16
-
-
7
-
-
-
-
1
-
-
-
-
4
1
-
-
1
15
-
-
-
-
-
1
1
-
15
15
722458
Baker
Rational design of stereoselec ...
Paraburkholderia xenovorans
J. Am. Chem. Soc.
134
507-513
2012
-
-
1
-
6
-
-
24
-
-
-
-
-
1
-
-
-
-
-
-
-
-
10
-
1
-
-
-
24
-
-
-
-
-
-
-
-
-
1
-
-
6
-
-
-
-
24
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
-
24
-
-
-
-
-
-
-
-
31
31
714244
Baker
Probing the molecular basis of ...
Paraburkholderia xenovorans
Biochemistry
50
3559-3569
2011
-
-
1
-
7
-
-
20
-
-
-
-
-
1
-
-
-
-
-
-
-
-
9
-
1
-
-
-
21
-
-
-
-
-
-
-
-
-
1
-
-
7
-
-
-
-
20
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
21
-
-
-
-
-
1
1
-
31
31
721651
Carere
Investigating the molecular de ...
Paraburkholderia xenovorans
Biochemistry
50
8407-8416
2011
-
-
1
-
10
-
-
19
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
1
-
-
-
19
-
-
-
-
-
-
-
-
-
1
-
-
10
-
-
-
-
19
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
19
-
-
-
-
-
1
1
-
-
-
702377
Wang
Comparison of two metal-depend ...
Paraburkholderia xenovorans
Biochemistry
49
3774-3782
2010
-
-
-
-
-
-
5
5
-
1
-
-
-
1
-
-
-
-
-
-
-
-
6
-
1
-
-
-
4
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
5
6
5
-
1
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
4
-
-
-
-
-
-
-
-
6
6
721615
Baker
Characterization of an aldolas ...
Paraburkholderia xenovorans
Biochemistry
48
6551-6558
2009
1
-
1
-
-
-
6
5
-
-
3
1
-
1
-
-
1
-
-
-
-
1
5
1
1
-
-
-
5
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
6
1
5
-
-
3
1
-
-
-
1
-
-
-
1
5
1
-
-
-
5
-
-
-
-
-
1
1
-
5
5