Protein-protein interactions and substrate channeling in orthologous and chimeric aldolase-dehydrogenase complexes
Baker, P.; Hillis, C.; Carere, J.; Seah, S.Y.K.; Biochemistry 51, 1942-1952 (2012) 
Data extracted from this reference:
Cloned(Commentary)
Cloned (Commentary)
Organism
chimeric complexes of Burkholderia xenovorans and Thermus thermophilus enzymes, TTHB246-BphJ and BphI-TTHB247 created by coexpression of the relevant genes in Escherichia coli using separate expression plasmids; separate expression of TTHB246 in recombinant Escherichia coli
Thermus thermophilus
Engineering
Protein Variants
Commentary
Organism
A324G
increase of channeling efficiency of propionaldehyde to a value comparable to that of acetaldehyde
Thermus thermophilus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
with 4-hydroxy-2-oxopentanoate at 50°C Km-value is not affected
Thermus thermophilus
0.037
-
4-hydroxy-2-oxohexanoate
TTHB246-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
0.041
-
4-hydroxy-2-oxopentanoate
TTHB246-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
0.045
-
4-hydroxy-2-oxopentanoate
app. Km-value, with Mn2+ chloride salt, pH 8.0, 25°C
Thermus thermophilus
0.055
-
4-hydroxy-2-oxopentanoate
app. Km-value, with Co2+ chloride salt, pH 8.0, 25°C
Thermus thermophilus
0.074
-
4-hydroxy-2-oxopentanoate
app. Km-value, with Ni2+ chloride salt, pH 8.0, 25°C
Thermus thermophilus
0.08
-
4-hydroxy-2-oxopentanoate
app. Km-value, with Mg2+ chloride salt, pH 8.0, 25°C
Thermus thermophilus
0.089
-
4-hydroxy-2-oxopentanoate
BphI-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
0.095
-
4-hydroxy-2-oxopentanoate
app. Km-value, with Ca2+ chloride salt, pH 8.0, 25°C
Thermus thermophilus
0.117
-
4-hydroxy-2-oxohexanoate
BphI-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
0.199
-
4-hydroxy-2-oxohexanoate
BphI-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
0.206
-
4-hydroxy-2-oxopentanoate
single TTHB246, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
0.21
-
4-hydroxy-2-oxohexanoate
single TTHB246, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
0.218
-
4-hydroxy-2-oxohexanoate
TTHB246-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
0.257
-
4-hydroxy-2-oxopentanoate
TTHB246-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
0.295
-
4-hydroxy-2-oxopentanoate
BphI-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37000
-
estimated by SDS-PAGE, in agreement with predicted molecular mass calculated from amino acid sequences
Thermus thermophilus
72000
-
native molecular mass of TTHB246, determined by gel filtration
Thermus thermophilus
78000
-
native molecular mass of TTHB246, determined by static light scattering
Thermus thermophilus
142000
-
native molecular mass of TTHB246-TTHB247 complex, determined by gel filtration
Thermus thermophilus
144000
-
molecular mass of TTHB246-BphJ chimeric complex, determined by gel filtration
Thermus thermophilus
145000
-
molecular mass of TTHB246-BphJ chimeric complex, determined by static light scattering
Thermus thermophilus
152000
-
native molecular mass of TTHB246-TTHB247 complex, determined by static light scattering
Thermus thermophilus
Organism
Organism
UniProt
Commentary
Textmining
Thermus thermophilus
Q53WI0
-
-
Purification (Commentary)
Purification (Commentary)
Organism
purified to homogeneity using Ni2+-NTA chromatography
Thermus thermophilus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(4S)-4-hydroxy-2-oxopentanoate
-
721659
Thermus thermophilus
acetaldehyde + pyruvate
-
-
-
?
4-hydroxy-2-oxohexanoate
-
721659
Thermus thermophilus
propanal + pyruvate
-
-
-
?
4-hydroxy-2-oxopentanoate
-
721659
Thermus thermophilus
acetaldehyde + pyruvate
-
-
-
?
additional information
no activity with (4R)-4-hydroxy-2-oxopentanoate
721659
Thermus thermophilus
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
enzyme can form a stable heterotetrameric complex with TTHB247 in vitro, consisting of two aldolase and two dehydrogenase subunits
Thermus thermophilus
Synonyms
Synonyms
Commentary
Organism
TTHB246
-
Thermus thermophilus
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
half-life in complex with TTHB247 is 10 h; half-life of chimeric TTHB246-BphJ complex is 2.66 h for TTHB246 activity and 1.62 for BphJ activity; half-life of single enzyme is 42 h
Thermus thermophilus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
with 4-hydroxy-2-oxopentanoate at 50°C turnover number is increased approximately 4fold
Thermus thermophilus
0.08
-
4-hydroxy-2-oxopentanoate
with Ca2+ chloride salt, pH 8.0, 25°C
Thermus thermophilus
0.13
-
4-hydroxy-2-oxopentanoate
with Mg2+ chloride salt, pH 8.0, 25°C
Thermus thermophilus
0.5
-
4-hydroxy-2-oxohexanoate
TTHB246-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
0.57
-
4-hydroxy-2-oxopentanoate
with Mn2+ chloride salt, pH 8.0, 25°C
Thermus thermophilus
0.6
-
4-hydroxy-2-oxopentanoate
TTHB246-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
0.79
-
4-hydroxy-2-oxopentanoate
with Ni2+ chloride salt, pH 8.0, 25°C
Thermus thermophilus
1
-
4-hydroxy-2-oxopentanoate
single TTHB246, 1 mM MnCl2, pH 8.0, 25°C; with Co2+ chloride salt, pH 8.0, 25°C
Thermus thermophilus
1.1
-
4-hydroxy-2-oxohexanoate
single TTHB246, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
2.1
-
4-hydroxy-2-oxohexanoate
BphI-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
2.2
-
4-hydroxy-2-oxohexanoate
TTHB246-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
2.3
-
4-hydroxy-2-oxopentanoate
BphI-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
2.4
-
4-hydroxy-2-oxopentanoate
TTHB246-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
3.9
-
4-hydroxy-2-oxohexanoate
BphI-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
4.1
-
4-hydroxy-2-oxopentanoate
BphI-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
Cloned(Commentary) (protein specific)
Commentary
Organism
chimeric complexes of Burkholderia xenovorans and Thermus thermophilus enzymes, TTHB246-BphJ and BphI-TTHB247 created by coexpression of the relevant genes in Escherichia coli using separate expression plasmids; separate expression of TTHB246 in recombinant Escherichia coli
Thermus thermophilus
Engineering (protein specific)
Protein Variants
Commentary
Organism
A324G
increase of channeling efficiency of propionaldehyde to a value comparable to that of acetaldehyde
Thermus thermophilus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
with 4-hydroxy-2-oxopentanoate at 50°C Km-value is not affected
Thermus thermophilus
0.037
-
4-hydroxy-2-oxohexanoate
TTHB246-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
0.041
-
4-hydroxy-2-oxopentanoate
TTHB246-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
0.045
-
4-hydroxy-2-oxopentanoate
app. Km-value, with Mn2+ chloride salt, pH 8.0, 25°C
Thermus thermophilus
0.055
-
4-hydroxy-2-oxopentanoate
app. Km-value, with Co2+ chloride salt, pH 8.0, 25°C
Thermus thermophilus
0.074
-
4-hydroxy-2-oxopentanoate
app. Km-value, with Ni2+ chloride salt, pH 8.0, 25°C
Thermus thermophilus
0.08
-
4-hydroxy-2-oxopentanoate
app. Km-value, with Mg2+ chloride salt, pH 8.0, 25°C
Thermus thermophilus
0.089
-
4-hydroxy-2-oxopentanoate
BphI-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
0.095
-
4-hydroxy-2-oxopentanoate
app. Km-value, with Ca2+ chloride salt, pH 8.0, 25°C
Thermus thermophilus
0.117
-
4-hydroxy-2-oxohexanoate
BphI-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
0.199
-
4-hydroxy-2-oxohexanoate
BphI-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
0.206
-
4-hydroxy-2-oxopentanoate
single TTHB246, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
0.21
-
4-hydroxy-2-oxohexanoate
single TTHB246, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
0.218
-
4-hydroxy-2-oxohexanoate
TTHB246-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
0.257
-
4-hydroxy-2-oxopentanoate
TTHB246-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
0.295
-
4-hydroxy-2-oxopentanoate
BphI-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37000
-
estimated by SDS-PAGE, in agreement with predicted molecular mass calculated from amino acid sequences
Thermus thermophilus
72000
-
native molecular mass of TTHB246, determined by gel filtration
Thermus thermophilus
78000
-
native molecular mass of TTHB246, determined by static light scattering
Thermus thermophilus
142000
-
native molecular mass of TTHB246-TTHB247 complex, determined by gel filtration
Thermus thermophilus
144000
-
molecular mass of TTHB246-BphJ chimeric complex, determined by gel filtration
Thermus thermophilus
145000
-
molecular mass of TTHB246-BphJ chimeric complex, determined by static light scattering
Thermus thermophilus
152000
-
native molecular mass of TTHB246-TTHB247 complex, determined by static light scattering
Thermus thermophilus
Purification (Commentary) (protein specific)
Commentary
Organism
purified to homogeneity using Ni2+-NTA chromatography
Thermus thermophilus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(4S)-4-hydroxy-2-oxopentanoate
-
721659
Thermus thermophilus
acetaldehyde + pyruvate
-
-
-
?
4-hydroxy-2-oxohexanoate
-
721659
Thermus thermophilus
propanal + pyruvate
-
-
-
?
4-hydroxy-2-oxopentanoate
-
721659
Thermus thermophilus
acetaldehyde + pyruvate
-
-
-
?
additional information
no activity with (4R)-4-hydroxy-2-oxopentanoate
721659
Thermus thermophilus
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
enzyme can form a stable heterotetrameric complex with TTHB247 in vitro, consisting of two aldolase and two dehydrogenase subunits
Thermus thermophilus
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
half-life in complex with TTHB247 is 10 h; half-life of chimeric TTHB246-BphJ complex is 2.66 h for TTHB246 activity and 1.62 for BphJ activity; half-life of single enzyme is 42 h
Thermus thermophilus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
with 4-hydroxy-2-oxopentanoate at 50°C turnover number is increased approximately 4fold
Thermus thermophilus
0.08
-
4-hydroxy-2-oxopentanoate
with Ca2+ chloride salt, pH 8.0, 25°C
Thermus thermophilus
0.13
-
4-hydroxy-2-oxopentanoate
with Mg2+ chloride salt, pH 8.0, 25°C
Thermus thermophilus
0.5
-
4-hydroxy-2-oxohexanoate
TTHB246-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
0.57
-
4-hydroxy-2-oxopentanoate
with Mn2+ chloride salt, pH 8.0, 25°C
Thermus thermophilus
0.6
-
4-hydroxy-2-oxopentanoate
TTHB246-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
0.79
-
4-hydroxy-2-oxopentanoate
with Ni2+ chloride salt, pH 8.0, 25°C
Thermus thermophilus
1
-
4-hydroxy-2-oxopentanoate
single TTHB246, 1 mM MnCl2, pH 8.0, 25°C; with Co2+ chloride salt, pH 8.0, 25°C
Thermus thermophilus
1.1
-
4-hydroxy-2-oxohexanoate
single TTHB246, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
2.1
-
4-hydroxy-2-oxohexanoate
BphI-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
2.2
-
4-hydroxy-2-oxohexanoate
TTHB246-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
2.3
-
4-hydroxy-2-oxopentanoate
BphI-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
2.4
-
4-hydroxy-2-oxopentanoate
TTHB246-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
3.9
-
4-hydroxy-2-oxohexanoate
BphI-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
4.1
-
4-hydroxy-2-oxopentanoate
BphI-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
General Information
General Information
Commentary
Organism
metabolism
together with TTHB247, EC 1.2.1.87, enzyme forms a bacterial aldolase-dehydrogenase complex catalyzing the last steps in the meta cleavage pathway of aromatic hydrocarbon degradation
Thermus thermophilus
General Information (protein specific)
General Information
Commentary
Organism
metabolism
together with TTHB247, EC 1.2.1.87, enzyme forms a bacterial aldolase-dehydrogenase complex catalyzing the last steps in the meta cleavage pathway of aromatic hydrocarbon degradation
Thermus thermophilus
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.8
-
4-hydroxy-2-oxopentanoate
with app. Km-value, with Ca2+ chloride salt, pH 8.0, 25°C
Thermus thermophilus
1.6
-
4-hydroxy-2-oxopentanoate
with app. Km-value, with Mg2+ chloride salt, pH 8.0, 25°C
Thermus thermophilus
4.9
-
4-hydroxy-2-oxopentanoate
single TTHB246, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
5.2
-
4-hydroxy-2-oxohexanoate
single TTHB246, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
7.8
-
4-hydroxy-2-oxopentanoate
BphI-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
9.3
-
4-hydroxy-2-oxopentanoate
TTHB246-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
10.1
-
4-hydroxy-2-oxohexanoate
TTHB246-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
10.6
-
4-hydroxy-2-oxohexanoate
BphI-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
11
-
4-hydroxy-2-oxopentanoate
with app. Km-value, with Ni2+ chloride salt, pH 8.0, 25°C
Thermus thermophilus
13
-
4-hydroxy-2-oxopentanoate
with app. Km-value, with Mn2+ chloride salt, pH 8.0, 25°C
Thermus thermophilus
13.5
-
4-hydroxy-2-oxohexanoate
TTHB246-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
14.6
-
4-hydroxy-2-oxopentanoate
TTHB246-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
19
-
4-hydroxy-2-oxopentanoate
with app. Km-value, with Co2+ chloride salt, pH 8.0, 25°C
Thermus thermophilus
33.3
-
4-hydroxy-2-oxohexanoate
BphI-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
46.1
-
4-hydroxy-2-oxopentanoate
BphI-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.8
-
4-hydroxy-2-oxopentanoate
with app. Km-value, with Ca2+ chloride salt, pH 8.0, 25°C
Thermus thermophilus
1.6
-
4-hydroxy-2-oxopentanoate
with app. Km-value, with Mg2+ chloride salt, pH 8.0, 25°C
Thermus thermophilus
4.9
-
4-hydroxy-2-oxopentanoate
single TTHB246, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
5.2
-
4-hydroxy-2-oxohexanoate
single TTHB246, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
7.8
-
4-hydroxy-2-oxopentanoate
BphI-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
9.3
-
4-hydroxy-2-oxopentanoate
TTHB246-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
10.1
-
4-hydroxy-2-oxohexanoate
TTHB246-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
10.6
-
4-hydroxy-2-oxohexanoate
BphI-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
11
-
4-hydroxy-2-oxopentanoate
with app. Km-value, with Ni2+ chloride salt, pH 8.0, 25°C
Thermus thermophilus
13
-
4-hydroxy-2-oxopentanoate
with app. Km-value, with Mn2+ chloride salt, pH 8.0, 25°C
Thermus thermophilus
13.5
-
4-hydroxy-2-oxohexanoate
TTHB246-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
14.6
-
4-hydroxy-2-oxopentanoate
TTHB246-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
19
-
4-hydroxy-2-oxopentanoate
with app. Km-value, with Co2+ chloride salt, pH 8.0, 25°C
Thermus thermophilus
33.3
-
4-hydroxy-2-oxohexanoate
BphI-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
46.1
-
4-hydroxy-2-oxopentanoate
BphI-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C
Thermus thermophilus
Other publictions for EC 4.1.3.43
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721659
Baker
Protein-protein interactions a ...
Thermus thermophilus
Biochemistry
51
1942-1952
2012
-
-
1
-
1
-
-
16
-
-
7
-
-
4
-
-
1
-
-
-
-
-
4
1
1
-
-
1
15
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
16
-
-
7
-
-
-
-
1
-
-
-
-
4
1
-
-
1
15
-
-
-
-
-
1
1
-
15
15
722458
Baker
Rational design of stereoselec ...
Paraburkholderia xenovorans
J. Am. Chem. Soc.
134
507-513
2012
-
-
1
-
6
-
-
24
-
-
-
-
-
1
-
-
-
-
-
-
-
-
10
-
1
-
-
-
24
-
-
-
-
-
-
-
-
-
1
-
-
6
-
-
-
-
24
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
-
24
-
-
-
-
-
-
-
-
31
31
714244
Baker
Probing the molecular basis of ...
Paraburkholderia xenovorans
Biochemistry
50
3559-3569
2011
-
-
1
-
7
-
-
20
-
-
-
-
-
1
-
-
-
-
-
-
-
-
9
-
1
-
-
-
21
-
-
-
-
-
-
-
-
-
1
-
-
7
-
-
-
-
20
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
21
-
-
-
-
-
1
1
-
31
31
721651
Carere
Investigating the molecular de ...
Paraburkholderia xenovorans
Biochemistry
50
8407-8416
2011
-
-
1
-
10
-
-
19
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
1
-
-
-
19
-
-
-
-
-
-
-
-
-
1
-
-
10
-
-
-
-
19
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
19
-
-
-
-
-
1
1
-
-
-
702377
Wang
Comparison of two metal-depend ...
Paraburkholderia xenovorans
Biochemistry
49
3774-3782
2010
-
-
-
-
-
-
5
5
-
1
-
-
-
1
-
-
-
-
-
-
-
-
6
-
1
-
-
-
4
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
5
6
5
-
1
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
4
-
-
-
-
-
-
-
-
6
6
721615
Baker
Characterization of an aldolas ...
Paraburkholderia xenovorans
Biochemistry
48
6551-6558
2009
1
-
1
-
-
-
6
5
-
-
3
1
-
1
-
-
1
-
-
-
-
1
5
1
1
-
-
-
5
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
6
1
5
-
-
3
1
-
-
-
1
-
-
-
1
5
1
-
-
-
5
-
-
-
-
-
1
1
-
5
5