Application | Comment | Organism |
---|---|---|
synthesis | preparation of (4S)-4-hydroxy-2-oxoglutarate at more than 95% enantiomeric excess by stereospecific synthesis from glyoxylate and pyruvate. Preparation of (4R)-4-hydroxy-2-oxoglutarate at 60% enantiomeric excess by selective cleavage of the (4S)-isomer of racemic 4-hydroxy-2-oxoglutarate leaving the (4R)-isomer in solution | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D,L-4-hydroxy-2-oxoglutarate | - |
Escherichia coli | pyruvate + glyoxylate + D-4-hydroxy-2-oxoglutarate | preferential cleavage of the L-isomer of racemic 4-hydroxy-2-oxoglutarate leaving the D-isomer in solution | r | |
additional information | enzyme shows high specificity for both glyoxylate and pyruvate | Escherichia coli | ? | - |
? | |
pyruvate + glyoxylate | - |
Escherichia coli | (4S)-4-hydroxy-2-oxoglutarate | i.e. L-4-hydroxy-2-oxoglutarate. Condensation of pyruvate and glyoxylate carried out at a relatively high concentration leads to a quantitative conversion of pyruvate and glyoxylate to (4S)-4-hydroxy-2-oxoglutarate | r |