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Literature summary for 4.1.3.4 extracted from
- Influence of multiple cysteines on human 3-hydroxy-3-methylglutaryl-CoA lyase activity and formation of inter-subunit adducts (2011), Arch. Biochem. Biophys., 511, 48-55.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| His-tagged version expressed in Escherichia coli BL21(DE3) | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C141S | strong inter-subunit dimer formation (SDS-PAGE, non-reducing conditions) | Homo sapiens |
| C170S | inter-subunit dimer formation not observed (SDS-PAGE, non-reducing conditions) | Homo sapiens |
| C170S/C174S | moderate inter-subunit dimer formation (SDS-PAGE, non-reducing conditions) | Homo sapiens |
| C170S/C266S | inter-subunit dimer formation not observed (SDS-PAGE, non-reducing conditions) | Homo sapiens |
| C170S/C323S | inter-subunit dimer formation not observed (SDS-PAGE, non-reducing conditions) | Homo sapiens |
| C174S | moderate inter-subunit dimer formation (SDS-PAGE, non-reducing conditions) | Homo sapiens |
| C197S | weak inter-subunit dimer formation (SDS-PAGE, non-reducing conditions) | Homo sapiens |
| C234S | weak inter-subunit dimer formation (SDS-PAGE, non-reducing conditions) | Homo sapiens |
| C266S | inter-subunit dimer formation not observed (SDS-PAGE, non-reducing conditions) | Homo sapiens |
| C307S | moderate inter-subunit dimer formation (SDS-PAGE, non-reducing conditions) | Homo sapiens |
| C323S | inter-subunit dimer formation not observed (SDS-PAGE, non-reducing conditions) | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.026 | - |
3-hydroxy-3-methylglutaryl-CoA | pH not specified in the publication, temperature not specified in the publication | Homo sapiens |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Homo sapiens | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P35914 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| immobilized metal ion affinity chromatography (Ni2+) | Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.123 | - |
C170S/C266S mutant protein, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
| 0.224 | - |
C266S mutant protein, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
| 1.06 | - |
C170S/C174S mutant protein, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
| 18.5 | - |
C170S/C323S mutant protein, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
| 21.2 | - |
C197S mutant protein, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
| 42 | - |
C141S mutant protein, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
| 42.1 | - |
C174S mutant protein, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
| 53.1 | - |
C170S mutant protein, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
| 123 | - |
wild type protein, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
| 128 | - |
C234S mutant protein, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
| 132 | - |
C307S mutant protein, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
| 132 | - |
C323S mutant protein, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-hydroxy-3-methylglutaryl-CoA | - |
Homo sapiens | acetoacetate + acetyl-CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | SDS-PAGE (reducing and non-reducing conditions) | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3-Hydroxy-3-methylglutaryl-CoA lyase | - |
Homo sapiens |
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