Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Pseudomonas mevalonii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-butynoyl-CoA | active-site inhibition, only recombinant wild-type, not C237 mutants, indication of active-site directed mutagenesis | Pseudomonas mevalonii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
hydroxymethylglutaryl-CoA | wild-type | Pseudomonas mevalonii | |
0.053 | - |
hydroxymethylglutaryl-CoA | C237A mutant | Pseudomonas mevalonii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas mevalonii | - |
recombinant wild-type enzyme and C237 mutants | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas mevalonii |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(S)-3-Hydroxy-3-methylglutaryl-CoA = acetyl-CoA + acetoacetate | C237 is crucial for catalysis | Pseudomonas mevalonii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0018 | 0.11 | C237 mutants | Pseudomonas mevalonii |
80 | - |
wild-type | Pseudomonas mevalonii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxy-3-methylglutaryl-CoA | - |
Pseudomonas mevalonii | acetoacetate + acetyl-CoA | - |
? |