Activating Compound | Comment | Organism | Structure |
---|---|---|---|
HsaG | the enzyme is active only in complex with the dehydrogenase HsaG | Mycobacterium tuberculosis |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
in complex with HsaG, sitting drop vapor diffusion method, using 18% (w/v) PEG 4000, 0.2 M MgCl2, 0.1 M Tris pH 8.5, 10% glycerol, and 10 mM sodium oxalate | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0044 | - |
(S)-4-hydroxy-2-oxopentanoate | in 100 mM HEPES (pH 8.0), 0.4 mM NADH, 1 mM MgCl2, at 25°C | Mycobacterium tuberculosis | |
0.0048 | - |
(S)-4-hydroxy-2-oxohexanoate | in 100 mM HEPES (pH 8.0), 0.4 mM NADH, 1 mM MgCl2, at 25°C | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | the specific activity is highest with Mn2+ (100%) | Mycobacterium tuberculosis | |
additional information | not stimulated by Ca2+, Cd2+, Zn2+, and Cu2+ | Mycobacterium tuberculosis | |
Ni2+ | 15.4% activity with 1 mM Ni2+ compared to Mn2+ | Mycobacterium tuberculosis | |
Ni2+ | 35.1% activity with 1 mM Mg2+ compared to Mn2+ | Mycobacterium tuberculosis | |
Ni2+ | 59.5% activity with 1 mM Co2+ compared to Mn2+ | Mycobacterium tuberculosis | |
Ni2+ | 76% activity with 1 mM Ni2+ compared to Mn2+ | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
2 * 38000, SDS-PAGE | Mycobacterium tuberculosis |
67000 | - |
gel filtration | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-4-hydroxy-2-oxohexanoate | Mycobacterium tuberculosis | - |
propanal + pyruvate | - |
? | |
(S)-4-hydroxy-2-oxohexanoate | Mycobacterium tuberculosis H37Rv | - |
propanal + pyruvate | - |
? | |
(S)-4-hydroxy-2-oxopentanoate | Mycobacterium tuberculosis | - |
ethanal + pyruvate | - |
? | |
(S)-4-hydroxy-2-oxopentanoate | Mycobacterium tuberculosis H37Rv | - |
ethanal + pyruvate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WMK5 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WMK5 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA column chromatography | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-4-hydroxy-2-oxohexanoate | - |
Mycobacterium tuberculosis | propanal + pyruvate | - |
? | |
(S)-4-hydroxy-2-oxohexanoate | - |
Mycobacterium tuberculosis H37Rv | propanal + pyruvate | - |
? | |
(S)-4-hydroxy-2-oxopentanoate | - |
Mycobacterium tuberculosis | ethanal + pyruvate | - |
? | |
(S)-4-hydroxy-2-oxopentanoate | - |
Mycobacterium tuberculosis H37Rv | ethanal + pyruvate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 38000, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
HsaF | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.38 | - |
(S)-4-hydroxy-2-oxohexanoate | in 100 mM HEPES (pH 8.0), 1 mM MgCl2, at 25°C | Mycobacterium tuberculosis | |
0.41 | - |
(S)-4-hydroxy-2-oxopentanoate | in 100 mM HEPES (pH 8.0), 1 mM MgCl2, at 25°C | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
79.1 | - |
(S)-4-hydroxy-2-oxohexanoate | in 100 mM HEPES (pH 8.0), 0.4 mM NADH, 1 mM MgCl2, at 25°C | Mycobacterium tuberculosis | |
93.1 | - |
(S)-4-hydroxy-2-oxopentanoate | in 100 mM HEPES (pH 8.0), 0.4 mM NADH, 1 mM MgCl2, at 25°C | Mycobacterium tuberculosis |