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Literature summary for 4.1.3.39 extracted from

  • Carere, J.; McKenna, S.E.; Kimber, M.S.; Seah, S.Y.
    Characterization of an aldolase-dehydrogenase complex from the cholesterol degradation pathway of Mycobacterium tuberculosis (2013), Biochemistry, 52, 3502-3511.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
HsaG the enzyme is active only in complex with the dehydrogenase HsaG Mycobacterium tuberculosis

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with HsaG, sitting drop vapor diffusion method, using 18% (w/v) PEG 4000, 0.2 M MgCl2, 0.1 M Tris pH 8.5, 10% glycerol, and 10 mM sodium oxalate Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0044
-
(S)-4-hydroxy-2-oxopentanoate in 100 mM HEPES (pH 8.0), 0.4 mM NADH, 1 mM MgCl2, at 25°C Mycobacterium tuberculosis
0.0048
-
(S)-4-hydroxy-2-oxohexanoate in 100 mM HEPES (pH 8.0), 0.4 mM NADH, 1 mM MgCl2, at 25°C Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ the specific activity is highest with Mn2+ (100%) Mycobacterium tuberculosis
additional information not stimulated by Ca2+, Cd2+, Zn2+, and Cu2+ Mycobacterium tuberculosis
Ni2+ 15.4% activity with 1 mM Ni2+ compared to Mn2+ Mycobacterium tuberculosis
Ni2+ 35.1% activity with 1 mM Mg2+ compared to Mn2+ Mycobacterium tuberculosis
Ni2+ 59.5% activity with 1 mM Co2+ compared to Mn2+ Mycobacterium tuberculosis
Ni2+ 76% activity with 1 mM Ni2+ compared to Mn2+ Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
2 * 38000, SDS-PAGE Mycobacterium tuberculosis
67000
-
gel filtration Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-4-hydroxy-2-oxohexanoate Mycobacterium tuberculosis
-
propanal + pyruvate
-
?
(S)-4-hydroxy-2-oxohexanoate Mycobacterium tuberculosis H37Rv
-
propanal + pyruvate
-
?
(S)-4-hydroxy-2-oxopentanoate Mycobacterium tuberculosis
-
ethanal + pyruvate
-
?
(S)-4-hydroxy-2-oxopentanoate Mycobacterium tuberculosis H37Rv
-
ethanal + pyruvate
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WMK5
-
-
Mycobacterium tuberculosis H37Rv P9WMK5
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-4-hydroxy-2-oxohexanoate
-
Mycobacterium tuberculosis propanal + pyruvate
-
?
(S)-4-hydroxy-2-oxohexanoate
-
Mycobacterium tuberculosis H37Rv propanal + pyruvate
-
?
(S)-4-hydroxy-2-oxopentanoate
-
Mycobacterium tuberculosis ethanal + pyruvate
-
?
(S)-4-hydroxy-2-oxopentanoate
-
Mycobacterium tuberculosis H37Rv ethanal + pyruvate
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 38000, SDS-PAGE Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
HsaF
-
Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.38
-
(S)-4-hydroxy-2-oxohexanoate in 100 mM HEPES (pH 8.0), 1 mM MgCl2, at 25°C Mycobacterium tuberculosis
0.41
-
(S)-4-hydroxy-2-oxopentanoate in 100 mM HEPES (pH 8.0), 1 mM MgCl2, at 25°C Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
79.1
-
(S)-4-hydroxy-2-oxohexanoate in 100 mM HEPES (pH 8.0), 0.4 mM NADH, 1 mM MgCl2, at 25°C Mycobacterium tuberculosis
93.1
-
(S)-4-hydroxy-2-oxopentanoate in 100 mM HEPES (pH 8.0), 0.4 mM NADH, 1 mM MgCl2, at 25°C Mycobacterium tuberculosis