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Literature summary for 4.1.3.36 extracted from
- Stabilization of the second oxyanion intermediate by 1,4-dihydroxy-2-naphthoyl-coenzyme a synthase of the menaquinone pathway: Spectroscopic evidence of the involvement of a conserved aspartic acid (2011), Biochemistry, 50, 5893-5904.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D163A | loss of the ligand binding ability | Escherichia coli |
| D163E | 14% 1,4-dihydroxy-2-naphthoyl-CoA synthetic activity | Escherichia coli |
| D163N | no 1,4-dihydroxy-2-naphthoyl-CoA synthetic activity | Escherichia coli |
| R91A | no 1,4-dihydroxy-2-naphthoyl-CoA synthetic activity | Escherichia coli |
| S161A | expressed in inclusion bodies and unavailable for the binding test | Escherichia coli |
| Y258F | no 1,4-dihydroxy-2-naphthoyl-CoA synthetic activity | Escherichia coli |
| Y94F | wild type level activity | Escherichia coli |
| Y97F | no 1,4-dihydroxy-2-naphthoyl-CoA synthetic activity | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-hydroxy-2-naphthoyl-CoA | - |
Escherichia coli |  |
| 2,3-dihydroxybenzoyl-CoA | - |
Escherichia coli | |
| 2,4-dihydroxybenzoyl-CoA | - |
Escherichia coli | |
| salicylyl-CoA | - |
Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, ion exchange chromatography (DEAE), gel filtration | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| o-succinylbenzoyl-CoA | - |
Escherichia coli | 1,4-dihydroxy-2-naphthoyl-CoA + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 1,4-dihydroxy-2-naphthoyl-coenzyme synthase | - |
Escherichia coli |
| MenB | - |
Escherichia coli |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0013 | - |
1-hydroxy-2-naphthoyl-CoA | pH 7,0, 23°C | Escherichia coli | |
| 0.0022 | - |
2,4-dihydroxybenzoyl-CoA | pH 7,0, 23°C | Escherichia coli | |
| 0.0031 | - |
2,3-dihydroxybenzoyl-CoA | pH 7,0, 23°C | Escherichia coli | |
| 0.006 | - |
salicylyl-CoA | pH 7,0, 23°C | Escherichia coli | |
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Release 2023.1 (January 2023)
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