BRENDA - Enzyme Database show
show all sequences of 4.1.3.34

Conversion of citrate synthase into citryl-CoA lyase as a result of mutation of the active-site aspartic acid residue to glutamic acid

Man, W.J.; Li, Y.; O'Connor, C.D.; Wilton, D.C.; Biochem. J. 280, 521-526 (1991)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
D362E
conversion of citrate synthase, EC 4.1.3.7, into citryl-CoA lyase, EC 4.1.3.34
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0367
-
oxaloacetate
wild-type enzyme
Escherichia coli
0.0565
-
oxaloacetate
mutant enzyme D362E
Escherichia coli
0.0718
-
acetyl-CoA
mutant enzyme D362E
Escherichia coli
0.345
-
acetyl-CoA
wild-type enzyme
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
mutant D362E, conversion of citrate synthase, EC 4.1.3.7, into citryl-CoA lyase, EC 4.1.3.34, as a result of mutation of the active-site Asp to Glu
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(3S)-citryl-CoA
-
33348
Escherichia coli
acetyl-CoA + oxaloacetate
-
33348
Escherichia coli
-
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
D362E
conversion of citrate synthase, EC 4.1.3.7, into citryl-CoA lyase, EC 4.1.3.34
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0367
-
oxaloacetate
wild-type enzyme
Escherichia coli
0.0565
-
oxaloacetate
mutant enzyme D362E
Escherichia coli
0.0718
-
acetyl-CoA
mutant enzyme D362E
Escherichia coli
0.345
-
acetyl-CoA
wild-type enzyme
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(3S)-citryl-CoA
-
33348
Escherichia coli
acetyl-CoA + oxaloacetate
-
33348
Escherichia coli
-
Other publictions for EC 4.1.3.34
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747993
Becerra
A phylogenetic approach to th ...
Hydrogenobacter thermophilus, Hydrogenobacter thermophilus DSM 6534
Int. Microbiol.
17
91-97
2014
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
728112
Wang
Functional characterization of ...
Streptomyces griseochromogenes
J. Microbiol.
50
770-776
2012
-
-
1
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
673369
Hugler
Autotrophic CO2 fixation via t ...
Hydrogenobacter hydrogenophilus, Sulfurihydrogenibium subterraneum, Thermocrinis ruber
Environ. Microbiol.
9
81-92
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
653277
Aoshima
A novel enzyme, citryl-CoA lya ...
Hydrogenobacter thermophilus, Hydrogenobacter thermophilus TK-6 / IAM 12695
Mol. Microbiol.
52
763-770
2004
-
-
1
-
-
-
3
3
-
-
2
2
-
16
-
-
1
-
-
-
3
-
6
1
-
-
1
1
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
3
-
-
2
2
-
-
-
1
-
-
3
-
6
1
-
-
1
1
1
1
-
-
-
-
-
-
-
-
33346
Bekal
Purification of Leuconostoc me ...
Leuconostoc mesenteroides
J. Bacteriol.
180
647-654
1998
-
-
1
-
-
-
-
-
-
-
1
1
-
1
-
-
1
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
33349
Bekal
-
Citrate lyases of lactic acid ...
Clostridium sphenoides, Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae, Lactococcus lactis subsp. lactis, Leuconostoc mesenteroides, Rubrivivax gelatinosus
Lait
78
3-10
1998
-
-
-
-
-
-
-
-
-
35
17
-
-
7
-
-
-
-
-
-
-
-
-
14
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
35
17
-
-
-
-
-
-
-
-
-
-
14
-
-
-
-
-
-
-
-
-
-
-
-
-
-
33348
Man
Conversion of citrate synthase ...
Escherichia coli
Biochem. J.
280
521-526
1991
-
-
-
-
1
-
-
4
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
33347
Hupperich
Citrate lyase from Klebsiella ...
Klebsiella pneumoniae, Klebsiella pneumoniae ATCC 13882
Eur. J. Biochem.
192
161-166
1990
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-