BRENDA - Enzyme Database
show all sequences of 4.1.3.30

Gene acquisition, duplication and metabolic specification: the evolution of fungal methylisocitrate lyases

Mueller, S.; Fleck, C.B.; Wilson, D.; Hummert, C.; Hube, B.; Brock, M.; Environ. Microbiol. 13, 1534-1548 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
His-tagged version expressed in Escherichia coli BL21(DE3)Rosetta2
Aspergillus fumigatus
Engineering
Amino acid exchange
Commentary
Organism
L521F
L521 contributes to substrate specificity
Aspergillus fumigatus
L521F/S523T
L521 and S523 contribute to substrate specificity
Aspergillus fumigatus
S523T
S523 contributes to substrate specificity
Aspergillus fumigatus
Inhibitors
Inhibitors
Commentary
Organism
Structure
isocitrate
-
Aspergillus fumigatus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0181
-
methylisocitrate
wild type protein, pH not specified in the publication, temperature not specified in the publication
Aspergillus fumigatus
0.0485
-
methylisocitrate
L521F/S523T mutant protein, pH not specified in the publication, temperature not specified in the publication
Aspergillus fumigatus
0.0674
-
methylisocitrate
S523T mutant protein, pH not specified in the publication, temperature not specified in the publication
Aspergillus fumigatus
0.0806
-
methylisocitrate
L521F mutant protein, pH not specified in the publication, temperature not specified in the publication
Aspergillus fumigatus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Aspergillus fumigatus
5739
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aspergillus fumigatus
-
-
-
Aspergillus fumigatus Af293
-
-
-
Purification (Commentary)
Commentary
Organism
immobilized metal ion affinity chromatography (Ni2+)
Aspergillus fumigatus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
activity not detectable for substrate isocitrate and wild type protein, pH not specified in the publication, temperature not specified in the publication
Aspergillus fumigatus
0.00055
-
substrate isocitrate, S523T mutant protein, pH not specified in the publication, temperature not specified in the publication
Aspergillus fumigatus
0.00105
-
substrate isocitrate, L521F mutant protein, pH not specified in the publication, temperature not specified in the publication
Aspergillus fumigatus
0.0033
-
substrate isocitrate, L521F/S523T mutant protein, pH not specified in the publication, temperature not specified in the publication
Aspergillus fumigatus
4.33
-
L521F/S523T mutant protein, pH not specified in the publication, temperature not specified in the publication
Aspergillus fumigatus
7.52
-
S523T mutant protein, pH not specified in the publication, temperature not specified in the publication
Aspergillus fumigatus
27.43
-
L521F mutant protein, pH not specified in the publication, temperature not specified in the publication
Aspergillus fumigatus
32.33
-
wild type protein, pH not specified in the publication, temperature not specified in the publication
Aspergillus fumigatus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
methylisocitrate
-
714846
Aspergillus fumigatus
succinate + pyruvate
-
-
-
?
methylisocitrate
-
714846
Aspergillus fumigatus Af293
succinate + pyruvate
-
-
-
?
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
additional information
-
additional information
0.258 mM isocitrate required to double the KM-value with methylisocitrate (wild type protein), 0.0598 mM isocitrate required to double the KM-value with methylisocitrate (L521F mutant protein), 0.0606 mM isocitrate required to double the KM-value with methylisocitrate (S523T mutant protein), 0.0119 mM isocitrate required to double the KM-value with methylisocitrate (L521F/L521F mutant protein)
Aspergillus fumigatus
Cloned(Commentary) (protein specific)
Commentary
Organism
His-tagged version expressed in Escherichia coli BL21(DE3)Rosetta2
Aspergillus fumigatus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
L521F
L521 contributes to substrate specificity
Aspergillus fumigatus
L521F/S523T
L521 and S523 contribute to substrate specificity
Aspergillus fumigatus
S523T
S523 contributes to substrate specificity
Aspergillus fumigatus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
isocitrate
-
Aspergillus fumigatus
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
additional information
-
additional information
0.258 mM isocitrate required to double the KM-value with methylisocitrate (wild type protein), 0.0598 mM isocitrate required to double the KM-value with methylisocitrate (L521F mutant protein), 0.0606 mM isocitrate required to double the KM-value with methylisocitrate (S523T mutant protein), 0.0119 mM isocitrate required to double the KM-value with methylisocitrate (L521F/L521F mutant protein)
Aspergillus fumigatus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0181
-
methylisocitrate
wild type protein, pH not specified in the publication, temperature not specified in the publication
Aspergillus fumigatus
0.0485
-
methylisocitrate
L521F/S523T mutant protein, pH not specified in the publication, temperature not specified in the publication
Aspergillus fumigatus
0.0674
-
methylisocitrate
S523T mutant protein, pH not specified in the publication, temperature not specified in the publication
Aspergillus fumigatus
0.0806
-
methylisocitrate
L521F mutant protein, pH not specified in the publication, temperature not specified in the publication
Aspergillus fumigatus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Aspergillus fumigatus
5739
-
Purification (Commentary) (protein specific)
Commentary
Organism
immobilized metal ion affinity chromatography (Ni2+)
Aspergillus fumigatus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
activity not detectable for substrate isocitrate and wild type protein, pH not specified in the publication, temperature not specified in the publication
Aspergillus fumigatus
0.00055
-
substrate isocitrate, S523T mutant protein, pH not specified in the publication, temperature not specified in the publication
Aspergillus fumigatus
0.00105
-
substrate isocitrate, L521F mutant protein, pH not specified in the publication, temperature not specified in the publication
Aspergillus fumigatus
0.0033
-
substrate isocitrate, L521F/S523T mutant protein, pH not specified in the publication, temperature not specified in the publication
Aspergillus fumigatus
4.33
-
L521F/S523T mutant protein, pH not specified in the publication, temperature not specified in the publication
Aspergillus fumigatus
7.52
-
S523T mutant protein, pH not specified in the publication, temperature not specified in the publication
Aspergillus fumigatus
27.43
-
L521F mutant protein, pH not specified in the publication, temperature not specified in the publication
Aspergillus fumigatus
32.33
-
wild type protein, pH not specified in the publication, temperature not specified in the publication
Aspergillus fumigatus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
methylisocitrate
-
714846
Aspergillus fumigatus
succinate + pyruvate
-
-
-
?
methylisocitrate
-
714846
Aspergillus fumigatus Af293
succinate + pyruvate
-
-
-
?
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
92.8
-
methylisocitrate
L521F/S523T mutant protein, pH not specified in the publication, temperature not specified in the publication
Aspergillus fumigatus
116
-
methylisocitrate
S523T mutant protein, pH not specified in the publication, temperature not specified in the publication
Aspergillus fumigatus
353
-
methylisocitrate
L521F mutant protein, pH not specified in the publication, temperature not specified in the publication
Aspergillus fumigatus
1850
-
methylisocitrate
wild type protein, pH not specified in the publication, temperature not specified in the publication
Aspergillus fumigatus
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
92.8
-
methylisocitrate
L521F/S523T mutant protein, pH not specified in the publication, temperature not specified in the publication
Aspergillus fumigatus
116
-
methylisocitrate
S523T mutant protein, pH not specified in the publication, temperature not specified in the publication
Aspergillus fumigatus
353
-
methylisocitrate
L521F mutant protein, pH not specified in the publication, temperature not specified in the publication
Aspergillus fumigatus
1850
-
methylisocitrate
wild type protein, pH not specified in the publication, temperature not specified in the publication
Aspergillus fumigatus
Other publictions for EC 4.1.3.30
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
749369
Lee
Glutamate mediated metabolic ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis BCG
Sci. Rep.
8
8506
2018
-
-
-
-
-
-
-
-
-
-
-
2
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
747131
Reddick
First biochemical characteriz ...
Bacillus subtilis 168, Bacillus subtilis
Biochemistry
56
5698-5711
2017
-
-
1
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
748248
Pulaganti
Design, synthesis, and evalua ...
Pseudomonas aeruginosa
J. Biomol. Struct. Dyn.
35
2509-2529
2017
-
-
-
-
-
-
14
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
14
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
748460
Jongkon
Probing the catalytic mechani ...
Mycobacterium tuberculosis
J. Phys. Chem. B
119
11473-11484
2015
-
-
-
-
1
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
728701
Eoh
Methylcitrate cycle defines th ...
Mycobacterium tuberculosis
Proc. Natl. Acad. Sci. USA
111
4976-4981
2014
-
-
-
-
-
-
1
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
728261
Dubey
Role of the methylcitrate cycl ...
Trichoderma atroviride, Trichoderma atroviride IMI206040
Microbiology
159
2492-2500
2013
-
-
1
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
3
3
1
-
-
728336
Limenitakis
The 2-methylcitrate cycle is i ...
Toxoplasma gondii
Mol. Microbiol.
87
894-908
2013
-
-
1
-
1
-
-
-
1
-
1
-
-
6
-
1
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
1
-
-
-
1
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
3
3
-
-
-
714846
Mueller
Gene acquisition, duplication ...
Aspergillus fumigatus, Aspergillus fumigatus Af293
Environ. Microbiol.
13
1534-1548
2011
-
-
1
-
3
-
1
4
1
-
-
-
-
15
-
-
1
-
-
-
8
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
3
-
-
1
1
4
1
-
-
-
-
-
-
1
-
-
8
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
4
4
703509
Lee
Roles of the glyoxylate and me ...
Fusarium graminearum, Fusarium graminearum Z03643
Eukaryot. Cell
8
1155-1164
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
681801
Upton
Role of the methylcitrate cycl ...
Mycolicibacterium smegmatis, Mycolicibacterium smegmatis mc(2)155 / ATCC 700084
Microbiology
153
3973-3982
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
23
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
681989
Munoz-Elias
Role of the methylcitrate cycl ...
Mycobacterium tuberculosis
Mol. Microbiol.
60
1109-1122
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
681995
Gould
Dual role of isocitrate lyase ...
Mycobacterium tuberculosis
Mol. Microbiol.
61
940-947
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
663709
Brock
Generation and phenotypic char ...
Aspergillus nidulans
Appl. Environ. Microbiol.
71
5465-5475
2005
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
664091
Liu
Crystal structures of 2-methyl ...
Escherichia coli
Biochemistry
44
2949-2962
2005
-
-
1
1
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
649628
Simanshu
Crystal structure of Salmonell ...
Salmonella enterica subsp. enterica serovar Typhimurium
Biochem. Biophys. Res. Commun.
311
193-201
2003
-
-
-
1
-
-
-
-
-
-
-
1
-
3
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
651720
Grimek
Residues C123 and D58 of the 2 ...
Salmonella enterica
J. Bacteriol.
185
4837-4843
2003
2
-
1
-
5
-
-
4
-
1
1
1
-
3
-
-
-
1
-
-
-
-
3
1
1
-
2
5
1
1
-
-
-
-
-
2
-
1
-
-
5
-
-
-
-
4
-
1
1
1
-
-
-
-
-
-
-
-
3
1
1
-
2
5
1
1
-
-
-
-
-
-
-
-
652912
Grimm
Crystal structure of 2-methyli ...
Escherichia coli
J. Mol. Biol.
328
609-621
2003
-
-
-
1
-
-
-
-
-
1
-
1
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
651100
Brock
2-Methylisocitrate lyases from ...
Aspergillus nidulans
Eur. J. Biochem.
268
3577-3586
2001
-
-
-
-
-
1
2
2
-
1
4
1
-
5
1
-
1
-
-
-
2
-
3
1
-
-
3
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
2
-
1
4
1
-
1
-
1
-
-
2
-
3
1
-
-
3
2
-
-
-
-
-
-
-
-
-
-
651674
Luttik
The Saccharomyces cerevisiae I ...
Saccharomyces cerevisiae
J. Bacteriol.
182
7007-7013
2000
-
-
1
-
-
-
-
-
2
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5348
Miyakoshi
-
Distribution of the methylcitr ...
Aspergillus niger, Diutina rugosa, Diutina rugosa IFO 0750, Mucor rouxianus, Mucor rouxianus IFO 5773, Neurospora crassa, Neurospora crassa IFO 6067, Rhizopus microsporus var. chinensis, Rhizopus microsporus var. chinensis IAM 6003, Ustilago crus-galli, Ustilago crus-galli F-B-6, Ustilago utriculosa, Ustilago utriculosa F-B-5, Wickerhamiella pararugosa, Wickerhamiella pararugosa IFO 0966
Agric. Biol. Chem.
51
2381-2387
1987
-
-
-
-
-
-
-
-
-
-
-
15
-
15
-
-
-
-
-
-
-
-
15
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
15
-
-
-
-
-
-
-
-
15
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5344
Uchiyama
Subcellular localization of th ...
Yarrowia lipolytica
Eur. J. Biochem.
125
523-527
1982
-
-
-
-
-
-
-
-
2
-
-
1
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5345
Tabuchi
-
Regulation of enzyme synthesis ...
Yarrowia lipolytica
Agric. Biol. Chem.
42
2381-2386
1978
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5346
Tabuchi
-
Purification and properties of ...
Yarrowia lipolytica
Agric. Biol. Chem.
41
169-174
1977
1
-
-
-
-
-
10
1
-
3
1
2
-
1
-
-
1
-
-
-
1
-
5
-
1
1
2
-
1
1
1
1
-
-
-
1
-
-
1
-
-
-
-
10
-
1
-
3
1
2
-
-
-
1
-
-
1
-
5
-
1
1
2
-
1
1
1
-
-
-
-
-
-
-
5347
Tabuchi
-
Distinction between isocitrate ...
Yarrowia lipolytica
Agric. Biol. Chem.
40
1863-1869
1976
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-