Cloned (Comment) | Organism |
---|---|
His-tagged version expressed in Escherichia coli BL21(DE3)Rosetta2 | Aspergillus fumigatus |
Protein Variants | Comment | Organism |
---|---|---|
L521F | L521 contributes to substrate specificity | Aspergillus fumigatus |
L521F/S523T | L521 and S523 contribute to substrate specificity | Aspergillus fumigatus |
S523T | S523 contributes to substrate specificity | Aspergillus fumigatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
isocitrate | - |
Aspergillus fumigatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0181 | - |
methylisocitrate | wild type protein, pH not specified in the publication, temperature not specified in the publication | Aspergillus fumigatus | |
0.0485 | - |
methylisocitrate | L521F/S523T mutant protein, pH not specified in the publication, temperature not specified in the publication | Aspergillus fumigatus | |
0.0674 | - |
methylisocitrate | S523T mutant protein, pH not specified in the publication, temperature not specified in the publication | Aspergillus fumigatus | |
0.0806 | - |
methylisocitrate | L521F mutant protein, pH not specified in the publication, temperature not specified in the publication | Aspergillus fumigatus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Aspergillus fumigatus | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus fumigatus | - |
- |
- |
Aspergillus fumigatus Af293 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography (Ni2+) | Aspergillus fumigatus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity not detectable for substrate isocitrate and wild type protein, pH not specified in the publication, temperature not specified in the publication | Aspergillus fumigatus |
0.00055 | - |
substrate isocitrate, S523T mutant protein, pH not specified in the publication, temperature not specified in the publication | Aspergillus fumigatus |
0.00105 | - |
substrate isocitrate, L521F mutant protein, pH not specified in the publication, temperature not specified in the publication | Aspergillus fumigatus |
0.0033 | - |
substrate isocitrate, L521F/S523T mutant protein, pH not specified in the publication, temperature not specified in the publication | Aspergillus fumigatus |
4.33 | - |
L521F/S523T mutant protein, pH not specified in the publication, temperature not specified in the publication | Aspergillus fumigatus |
7.52 | - |
S523T mutant protein, pH not specified in the publication, temperature not specified in the publication | Aspergillus fumigatus |
27.43 | - |
L521F mutant protein, pH not specified in the publication, temperature not specified in the publication | Aspergillus fumigatus |
32.33 | - |
wild type protein, pH not specified in the publication, temperature not specified in the publication | Aspergillus fumigatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methylisocitrate | - |
Aspergillus fumigatus | succinate + pyruvate | - |
? | |
methylisocitrate | - |
Aspergillus fumigatus Af293 | succinate + pyruvate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
methylisocitrate lyase | - |
Aspergillus fumigatus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | 0.258 mM isocitrate required to double the KM-value with methylisocitrate (wild type protein), 0.0598 mM isocitrate required to double the KM-value with methylisocitrate (L521F mutant protein), 0.0606 mM isocitrate required to double the KM-value with methylisocitrate (S523T mutant protein), 0.0119 mM isocitrate required to double the KM-value with methylisocitrate (L521F/L521F mutant protein) | Aspergillus fumigatus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
92.8 | - |
methylisocitrate | L521F/S523T mutant protein, pH not specified in the publication, temperature not specified in the publication | Aspergillus fumigatus | |
116 | - |
methylisocitrate | S523T mutant protein, pH not specified in the publication, temperature not specified in the publication | Aspergillus fumigatus | |
353 | - |
methylisocitrate | L521F mutant protein, pH not specified in the publication, temperature not specified in the publication | Aspergillus fumigatus | |
1850 | - |
methylisocitrate | wild type protein, pH not specified in the publication, temperature not specified in the publication | Aspergillus fumigatus |