Literature summary for 4.1.3.30 extracted from

Liu, S.; Lu, Z.; Han, Y.; Melamud, E.; Dunaway-Mariano, D.; Herzberg, O.
Crystal structures of 2-methylisocitrate lyase in complex with product and with isocitrate inhibitor provide insight into lyase substrate specificity, catalysis and evolution (2005), Biochemistry, 44, 2949-2962.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystals of the inactive mutant C123S MICL in complex with isocitrate are obtained at room temperature by vapor diffusion in hanging drops. A crystal of C123S MICL in complex with pyruvate and succinate was obtained by overnight incubation of a C123S MICL/isocitrate complex crystal in a solution containing 35% PEG3350, 100 mM pyruvate and 100 mM succinate. This crystal also belongs to space group C2	Escherichia coli

Crystallization (Comment)

Organism

crystals of the inactive mutant C123S MICL in complex with isocitrate are obtained at room temperature by vapor diffusion in hanging drops. A crystal of C123S MICL in complex with pyruvate and succinate was obtained by overnight incubation of a C123S MICL/isocitrate complex crystal in a solution containing 35% PEG3350, 100 mM pyruvate and 100 mM succinate. This crystal also belongs to space group C2

Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.024	-	(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate	-	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P77541	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate	a catalytic mechanism is proposed that involves an R-carboxy-carbanion intermediate/transition state, which is consistent with previous stereochemical experiments showing inversion of configuration at the C(3) of 2-methyl-isocitrate	Escherichia coli	Pyruvate + succinate	-	?

Synonyms

Synonyms	Comment	Organism
MICL	-	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
19	-	(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate	-	Escherichia coli