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Literature summary for 4.1.3.27 extracted from
- The fused anthranilate synthase from Streptomyces venezuelae functions as a monomer (2015), Mol. Cell. Biochem., 400, 9-15 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Streptomyces venezuelae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| tryptophan | the enzyme is competitively but non-cooperatively inhibited by tryptophan | Streptomyces venezuelae |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00084 | - |
L-glutamine | at pH 7.5 and 30°C | Streptomyces venezuelae | |
| 0.0082 | - |
chorismate | at pH 7.5 and 30°C | Streptomyces venezuelae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | - |
gel filtration | Streptomyces venezuelae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chorismate + L-glutamine | Streptomyces venezuelae | - |
anthranilate + pyruvate + L-glutamate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces venezuelae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Streptomyces venezuelae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chorismate + L-glutamine | - |
Streptomyces venezuelae | anthranilate + pyruvate + L-glutamate | - |
? | |
| additional information | the enzyme does not utilize ammonia as a substrate | Streptomyces venezuelae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 67000, SDS-PAGE | Streptomyces venezuelae |
| monomer | 1 * 66600, laser light scattering | Streptomyces venezuelae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TrpE | - |
Streptomyces venezuelae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Streptomyces venezuelae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.57 | - |
L-glutamine | at pH 7.5 and 30°C | Streptomyces venezuelae | |
| 0.57 | - |
chorismate | at pH 7.5 and 30°C | Streptomyces venezuelae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Streptomyces venezuelae |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0111 | - |
tryptophan | at pH 7.5 and 30°C | Streptomyces venezuelae | |
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