Cloned (Comment) | Organism |
---|---|
gene trpED | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of an L-tryptophan overproducing Escherichia coli strain from strain JM109 by defined genetic modification methodology via elimination of feedback inhibitions of 3-deoxy-D-arabinoheptulosonate 7-phosphate synthase (AroF) and anthranilate synthase by site-directed mutagenesis. Expression of deregulated AroF and TrpED is achieved by using a temperature-inducible expression plasmid pSV. Transcriptional regulation of trp repressor is removed by deleting trpR. The pathway for L-Trp degradation is removed by deleting tnaA. L-Phenylalanine and L-tyrosine biosynthesis pathways that compete with L-tryptophan biosynthesis are blocked by deleting their critical genes, pheA and tyrA. L-Phe, L-Tyr, and L-Trp accumulate in the culture medium, phenotype, overview | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene trpED | - |
Escherichia coli W3110 / ATCC 27325 | - |
gene trpED | - |
Synonyms | Comment | Organism |
---|---|---|
ANTA synthase | - |
Escherichia coli |
TrpED | - |
Escherichia coli |