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Literature summary for 4.1.3.27 extracted from

  • Zhao, Z.J.; Zou, C.; Zhu, Y.X.; Dai, J.; Chen, S.; Wu, D.; Wu, J.; Chen, J.
    Development of L-tryptophan production strains by defined genetic modification in Escherichia coli (2011), J. Ind. Microbiol. Biotechnol., 38, 1921-1929.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene trpED Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information construction of an L-tryptophan overproducing Escherichia coli strain from strain JM109 by defined genetic modification methodology via elimination of feedback inhibitions of 3-deoxy-D-arabinoheptulosonate 7-phosphate synthase (AroF) and anthranilate synthase by site-directed mutagenesis. Expression of deregulated AroF and TrpED is achieved by using a temperature-inducible expression plasmid pSV. Transcriptional regulation of trp repressor is removed by deleting trpR. The pathway for L-Trp degradation is removed by deleting tnaA. L-Phenylalanine and L-tyrosine biosynthesis pathways that compete with L-tryptophan biosynthesis are blocked by deleting their critical genes, pheA and tyrA. L-Phe, L-Tyr, and L-Trp accumulate in the culture medium, phenotype, overview Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
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gene trpED
-
Escherichia coli W3110 / ATCC 27325
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gene trpED
-

Synonyms

Synonyms Comment Organism
ANTA synthase
-
Escherichia coli
TrpED
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Escherichia coli