Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.3.27 extracted from

  • Bode, R.; Birnbaum, D.
    Die Enzyme der Biosynthese aromatischer Aminosäuren bei Hansenula henricii: Charakterisierung der Anthranilat-synthase (1978), Biochem. Physiol. Pflanz., 172, 245-253.
No PubMed abstract available
Search for more literature for 4.1.3.27

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.023
-
 chorismate Gln-dependent enzyme that is composed of 2 NH4+-dependent subunits and of 2 amidotransferase subunits (I2II2) Ogataea henricii
0.094
-
 Gln Gln-dependent enzyme that is composed of 2 NH4+-dependent subunits and of 2 amidotransferase subunits (I2II2) Ogataea henricii
1.2
-
 NH4+ NH4+-dependent enzyme, component I Ogataea henricii
3.1
-
 chorismate NH4+-dependent enzyme, component I Ogataea henricii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Ogataea henricii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
 2 * 20000 + 2 * 50000, Gln-dependent enzyme, composed of 2 NH4+-dependent subunits and of 2 amidotransferase subunits, I2II2, gel filtration Ogataea henricii
50000
-
 NH4-dependent enzyme, i.e. component I, gel filtration Ogataea henricii
160000
-
 Gln-dependent enzyme composed of 2 NH4+-dependent subunits and of 2 amidotransferase subunits (I2II2), gel filtration Ogataea henricii

Organism

Organism UniProt Comment Textmining
Ogataea henricii
-
 CCY 38-10-2, the organism contains an NH4-dependent enzyme (component I) and a Gln-dependent enzyme that is composed of 2 NH4+-dependent subunits and of 2 amidotransferase subunits (I2II2)
-

Purification (Commentary)

Purification (Comment) Organism
-
 Ogataea henricii

Reaction

Reaction Comment Organism Reaction ID
chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate sequential mechanism Ogataea henricii
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
43
-
-
 Ogataea henricii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chorismate + L-Gln
-
 Ogataea henricii anthranilate + pyruvate + L-glutamate
-
 ?
chorismate + NH4+
-
 Ogataea henricii ?
-
 ?

Subunits

Subunits Comment Organism
More the organism contains an NH4-dependent enzyme, i.e. component I, and a Gln-dependent enzyme that is composed of 2 NH4+-dependent subunits and of 2 amidotransferase subunits, i.e. I2II2 Ogataea henricii
tetramer 2 * 20000 + 2 * 50000, Gln-dependent enzyme, composed of 2 NH4+-dependent subunits and of 2 amidotransferase subunits, I2II2, gel filtration Ogataea henricii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7
-
 Gln-dependent reaction Ogataea henricii
7.3
-
 NH4+-dependent enzyme Ogataea henricii