BRENDA - Enzyme Database
show all sequences of 4.1.3.26

The bifunctional role of LiuE from Pseudomonas aeruginosa, displays additionally HIHG-CoA lyase enzymatic activity

Chavez-Aviles, M.; Diaz-Perez, A.L.; Campos-Garcia, J.; Mol. Biol. Rep. 37, 1787-1791 (2010)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
His-tagged protein expressed in Escherichia coli JM101
Pseudomonas aeruginosa PAO1
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33000
-
x * 33000, SDS-PAGE
Pseudomonas aeruginosa PAO1
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas aeruginosa PAO1
Q9I2A0
-
-
Purification (Commentary)
Purification (Commentary)
Organism
by affinity chromatography, more than 98% purified
Pseudomonas aeruginosa PAO1
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3-hydroxy-3-isohexenylglutaryl-CoA
-
705632
Pseudomonas aeruginosa PAO1
7-methyl-3-oxo-6-octenyl-CoA + acetate
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 33000, SDS-PAGE
Pseudomonas aeruginosa PAO1
Synonyms
Synonyms
Commentary
Organism
3-hydroxy-3-isohexenylglutaryl-CoA lyase
-
Pseudomonas aeruginosa PAO1
HIHG-CoA lyase
-
Pseudomonas aeruginosa PAO1
LiuE
-
Pseudomonas aeruginosa PAO1
Cloned(Commentary) (protein specific)
Commentary
Organism
His-tagged protein expressed in Escherichia coli JM101
Pseudomonas aeruginosa PAO1
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33000
-
x * 33000, SDS-PAGE
Pseudomonas aeruginosa PAO1
Purification (Commentary) (protein specific)
Commentary
Organism
by affinity chromatography, more than 98% purified
Pseudomonas aeruginosa PAO1
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3-hydroxy-3-isohexenylglutaryl-CoA
-
705632
Pseudomonas aeruginosa PAO1
7-methyl-3-oxo-6-octenyl-CoA + acetate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 33000, SDS-PAGE
Pseudomonas aeruginosa PAO1
General Information
General Information
Commentary
Organism
metabolism
LiuE displays a bifunctional role in both the leucine/isovalerate and acyclic terpenes catabolic pathways, LiuE shows enzymatic activity of HMG-CoA lyase and also HIHG-CoA lyase activity
Pseudomonas aeruginosa PAO1
General Information (protein specific)
General Information
Commentary
Organism
metabolism
LiuE displays a bifunctional role in both the leucine/isovalerate and acyclic terpenes catabolic pathways, LiuE shows enzymatic activity of HMG-CoA lyase and also HIHG-CoA lyase activity
Pseudomonas aeruginosa PAO1
Other publictions for EC 4.1.3.26
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
705632
Chavez-Aviles
The bifunctional role of LiuE ...
Pseudomonas aeruginosa PAO1
Mol. Biol. Rep.
37
1787-1791
2010
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5337
Seubert
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Untersuchungen ber den bakter ...
Pseudomonas citronellolis
Biochem. Z.
341
23-34
1964
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