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Literature summary for 4.1.3.24 extracted from

  • Gonzalez, J.M.; Marti-Arbona, R.; Chen, J.C.; Unkefer, C.J.
    Structure of Methylobacterium extorquens malyl-CoA lyase CoA-substrate binding correlates with domain shift (2017), Acta Crystallogr. Sect. F, 73, 79-85 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.3.24

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Arctic Express (DE3) cells Methylorubrum extorquens

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 0.1 M HEPES pH 7.5, 30%(w/v) PEG 400, 0.2 M MgCl2 Methylorubrum extorquens

Inhibitors

Inhibitors Comment Organism Structure
(2R)-4-malyl-CoA competitive inhibitor Methylorubrum extorquens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on Methylorubrum extorquens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2S)-4-malyl-CoA Methylorubrum extorquens
-
 acetyl-CoA + glyoxylate
-
 r
acetyl-CoA + glyoxylate Methylorubrum extorquens
-
 (2S)-4-malyl-CoA
-
 r
propionyl-CoA + glyoxylate Methylorubrum extorquens
-
 (2S)-4-(beta-methyl)-malyl-CoA
-
 r

Organism

Organism UniProt Comment Textmining
Methylorubrum extorquens C5B113
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Superdex 200 gel filtration Methylorubrum extorquens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S)-4-malyl-CoA
-
 Methylorubrum extorquens acetyl-CoA + glyoxylate
-
 r
acetyl-CoA + glyoxylate
-
 Methylorubrum extorquens (2S)-4-malyl-CoA
-
 r
propionyl-CoA + glyoxylate
-
 Methylorubrum extorquens (2S)-4-(beta-methyl)-malyl-CoA
-
 r

Subunits

Subunits Comment Organism
homohexamer 6 * 34000, calculated from amino acid sequence Methylorubrum extorquens

Synonyms

Synonyms Comment Organism
MCL
-
 Methylorubrum extorquens