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Literature summary for 4.1.3.17 extracted from
- 4-Hydroxy-4-methyl-2-ketoglutarate aldolase from Pseudomonas putida (1982), Methods Enzymol., 90, 272-276.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.067 | - |
4-hydroxy-4-methyl-2-oxoglutarate | - |
Pseudomonas putida |  |
| 0.29 | - |
4-carboxy-4-hydroxy-2-oxoadipate | - |
Pseudomonas putida | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activates | Pseudomonas putida | |
| Mg2+ | Mn2+ or Mg2+ required | Pseudomonas putida | |
| Mg2+ | Km: 0.17 mM MgCl2 | Pseudomonas putida | |
| Mn2+ | Mn2+ or Mg2+ required | Pseudomonas putida | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 150000 | - |
equilibrium sedimentation | Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas putida |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 67 | - |
- |
Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-carboxy-4-hydroxy-2-oxoadipate | - |
Pseudomonas putida | pyruvate + oxaloacetate | - |
? | |
| 4-hydroxy-4-methyl-2-oxoglutarate | - |
Pseudomonas putida | pyruvate + pyruvate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | - |
L-4-carboxy-4-hydroxy-2-oxoadipate, without phosphate | Pseudomonas putida |
| 8 | - |
D-4-carboxy-4-hydroxy-2-oxoadipate, without phosphate | Pseudomonas putida |
| 8.2 | - |
L-4-carboxy-4-hydroxy-2-oxoadipate, with 3 mM phosphate | Pseudomonas putida |
| 8.3 | - |
DL-4-hydroxy-4-methyl-2-oxoglutarate, without phosphate | Pseudomonas putida |
| 8.6 | - |
D-4-carboxy-4-hydroxy-2-oxoadipate, with 3 mM phosphate | Pseudomonas putida |
| 8.8 | - |
oxaloacetate, without phosphate | Pseudomonas putida |
| 8.9 | - |
DL-4-hydroxy-4-methyl-2-oxoglutarate or oxaloacetate, with 3 mM phosphate | Pseudomonas putida |
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