BRENDA - Enzyme Database
show all sequences of 4.1.3.16

Extended substrate range of thiamine diphosphate-dependent MenD enzyme by coupling of two C-C-bonding reactions

Schapfl, M.; Baier, S.; Fries, A.; Ferlaino, S.; Waltzer, S.; Mueller, M.; Sprenger, G.A.; Appl. Microbiol. Biotechnol. 102, 8359-8372 (2018)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli BL21(DE3) cells
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
4-Hydroxy-2-oxoglutarate
Escherichia coli
-
Pyruvate + glyoxylate
-
-
r
Organism
Organism
UniProt
Commentary
Textmining
Escherichia coli
P0A955
-
-
Purification (Commentary)
Purification (Commentary)
Organism
Ni-NTA column chromatography
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
4-Hydroxy-2-oxoglutarate
-
746867
Escherichia coli
Pyruvate + glyoxylate
-
-
-
r
Synonyms
Synonyms
Commentary
Organism
2-oxo-3-deoxy-6-phosphogluconate aldolase
-
Escherichia coli
eda
-
Escherichia coli
Entner-Doudoroff aldolase
-
Escherichia coli
KDPGlc aldolase
-
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
4-Hydroxy-2-oxoglutarate
Escherichia coli
-
Pyruvate + glyoxylate
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA column chromatography
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
4-Hydroxy-2-oxoglutarate
-
746867
Escherichia coli
Pyruvate + glyoxylate
-
-
-
r
Other publictions for EC 4.1.3.16
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746867
Schapfl
Extended substrate range of t ...
Escherichia coli
Appl. Microbiol. Biotechnol.
102
8359-8372
2018
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4
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748294
Mdimegh
HOGA1 gene mutations of prima ...
Homo sapiens
J. Clin. Lab. Anal.
31
e22053
2017
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2
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1
1
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747772
MacDonald
Cellular degradation of 4-hyd ...
Homo sapiens
FEBS Lett.
590
1467-1476
2016
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1
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1
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1
1
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746641
Huang
Use of a novel microtitration ...
Bos taurus
Acta Crystallogr. Sect. F
70
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2014
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1
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4
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1
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1
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1
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1
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721727
Riedel
4-Hydroxy-2-oxoglutarate aldol ...
Homo sapiens
Biochim. Biophys. Acta
1822
1544-1552
2012
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9
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1
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1
1
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9
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2
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1
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1
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Williams
The enzyme 4-hydroxy-2-oxoglut ...
Homo sapiens
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27
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2012
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1
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723542
Riedel
Structural and biochemical stu ...
Homo sapiens
PLoS ONE
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2011
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2
2
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10
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1
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1
11
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10
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2
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2
2
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10
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Mutations in DHDPSL are respon ...
Homo sapiens
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Dekker
2-keto-4-Hydroxyglutarate aldo ...
Bos taurus
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1
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5
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2
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1
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7
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2
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1
6
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1
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5
1
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Rattus norvegicus
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1
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10
3
1
2
1
1
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1
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2
2
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1
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5252
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Rattus norvegicus
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5250
Grady
Steady-state kinetics and inhi ...
Bos taurus
Biochemistry
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1981
2
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13
1
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1
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3
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13
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2
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1
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5247
Lane
Sulfhydryl groups in relation ...
Bos taurus
Biochim. Biophys. Acta
481
212-221
1977
-
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2
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1
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2
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2
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1
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5246
Hansen
Inactivation of bovine liver 2 ...
Bos taurus
Biochemistry
15
2912-2917
1976
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1
1
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1
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3
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5243
Dekker
2-keto-4-Hydroxyglutarate aldo ...
Bos taurus
Methods Enzymol.
42C
280-285
1975
-
-
-
-
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-
1
3
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1
1
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2
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1
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2
1
1
2
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1
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1
1
2
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2159
Adams
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Enzymes and intermediates of h ...
Rattus norvegicus
Methods Enzymol.
17B
266-306
1971
-
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2
2
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1
1
1
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1
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1
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4
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1
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1
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4
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1
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5238
Lane
2-keto-4-Hydroxybutyrate aldol ...
Bos taurus, Mammalia
Biochemistry
10
1353-1364
1971
-
-
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4
2
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2
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2
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2
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1
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1
2
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6
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1
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1
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2
2
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4
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2
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2
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2
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1
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1
2
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6
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1
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1
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2
2
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5241
Kobes
Variant properties of bovine l ...
Bos taurus
Biochim. Biophys. Acta
251
238-250
1971
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1
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7
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1
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5237
Kuratomi
The metabolism of gamma-hydrox ...
Rattus norvegicus
Biochim. Biophys. Acta
78
617-628
1963
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7
3
1
1
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