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Literature summary for 4.1.3.16 extracted from

  • Riedel, T.J.; Johnson, L.C.; Knight, J.; Hantgan, R.R.; Holmes, R.P.; Lowther, W.T.
    Structural and biochemical studies of human 4-hydroxy-2-oxoglutarate aldolase: implications for hydroxyproline metabolism in primary hyperoxaluria (2011), PLoS ONE, 6, e26021.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
to 1.97 A resolution, crystal structure of enzyme bound to pyruvate. Modeling of the 4-hydroxy-2-oxoglutarate-Schiff base intermediate and kinetic analyses of site-directed mutants support the importance of Lys196 as the nucleophile, Tyr168 and Ser77 as components of a proton relay, and Asn78 and Ser198 as unique residues that facilitate substrate binding Homo sapiens

Protein Variants

Protein Variants Comment Organism
K196A complete loss of activity Homo sapiens
N78A 20% of wild-type activity Homo sapiens
N78Q 4% of wild-type activity Homo sapiens
N78T 45% of wild-type activity Homo sapiens
S198A 9% of wild-type activity Homo sapiens
S198T 18% of wild-type activity Homo sapiens
S77A 5% of wild-type activity Homo sapiens
S77T 2% of wild-type activity Homo sapiens
S77V 2% of wild-type activity Homo sapiens
Y140F 122% of wild-type activity Homo sapiens
Y168F complete loss of activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
4-hydroxy-2-oxoglutarate mutant Y140F, pH 8.5, 37°C Homo sapiens
0.011
-
4-hydroxy-2-oxoglutarate wild-type, pH 8.5, 37°C Homo sapiens
0.016
-
4-hydroxy-2-oxoglutarate mutant N78T, pH 8.5, 37°C Homo sapiens
0.044
-
4-hydroxy-2-oxoglutarate mutant S198A, pH 8.5, 37°C Homo sapiens
0.058
-
4-hydroxy-2-oxoglutarate mutant S77T, pH 8.5, 37°C Homo sapiens
0.066
-
4-hydroxy-2-oxoglutarate mutant N78A, pH 8.5, 37°C Homo sapiens
0.077
-
4-hydroxy-2-oxoglutarate mutant S198T, pH 8.5, 37°C Homo sapiens
0.082
-
4-hydroxy-2-oxoglutarate mutant S77V, pH 8.5, 37°C Homo sapiens
0.084
-
4-hydroxy-2-oxoglutarate mutant S77A, pH 8.5, 37°C Homo sapiens
0.281
-
4-hydroxy-2-oxoglutarate mutant N78Q, pH 8.5, 37°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35249
-
and dimer, in equilibrium, 4 * 35249, calculated Homo sapiens
35249
-
and tetramer, in equilibrium, 2 * 35249, calculated Homo sapiens
97200
-
sedimentation equilibrium centrifugation Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q86XE5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-Hydroxy-2-oxoglutarate
-
Homo sapiens Pyruvate + glyoxylate
-
r
additional information enzyme performs a retro-aldol cleavage reaction reminiscent of the trimeric 2-keto-3-deoxy-6-phosphogluconate aldolases Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
dimer and tetramer, in equilibrium, 2 * 35249, calculated Homo sapiens
tetramer and dimer, in equilibrium, 4 * 35249, calculated Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.8
-
4-hydroxy-2-oxoglutarate mutant S77T, pH 8.5, 37°C Homo sapiens
1.2
-
4-hydroxy-2-oxoglutarate mutant S77V, pH 8.5, 37°C Homo sapiens
2.4
-
4-hydroxy-2-oxoglutarate mutant S198A, pH 8.5, 37°C Homo sapiens
2.5
-
4-hydroxy-2-oxoglutarate mutant S77A, pH 8.5, 37°C Homo sapiens
4
-
4-hydroxy-2-oxoglutarate mutant N78T, pH 8.5, 37°C Homo sapiens
5.2
-
4-hydroxy-2-oxoglutarate mutant Y140F, pH 8.5, 37°C Homo sapiens
6.1
-
4-hydroxy-2-oxoglutarate wild-type, pH 8.5, 37°C Homo sapiens
6.2
-
4-hydroxy-2-oxoglutarate mutant N78Q, pH 8.5, 37°C Homo sapiens
7.6
-
4-hydroxy-2-oxoglutarate mutant N78A, pH 8.5, 37°C Homo sapiens
7.8
-
4-hydroxy-2-oxoglutarate mutant S198T, pH 8.5, 37°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
14
-
4-hydroxy-2-oxoglutarate mutant S77T, pH 8.5, 37°C Homo sapiens
15
-
4-hydroxy-2-oxoglutarate mutant S77V, pH 8.5, 37°C Homo sapiens
22
-
4-hydroxy-2-oxoglutarate mutant N78Q, pH 8.5, 37°C Homo sapiens
30
-
4-hydroxy-2-oxoglutarate mutant S77A, pH 8.5, 37°C Homo sapiens
55
-
4-hydroxy-2-oxoglutarate mutant S198A, pH 8.5, 37°C Homo sapiens
101
-
4-hydroxy-2-oxoglutarate mutant S198T, pH 8.5, 37°C Homo sapiens
115
-
4-hydroxy-2-oxoglutarate mutant N78A, pH 8.5, 37°C Homo sapiens
250
-
4-hydroxy-2-oxoglutarate mutant N78T, pH 8.5, 37°C Homo sapiens
555
-
4-hydroxy-2-oxoglutarate wild-type, pH 8.5, 37°C Homo sapiens
650
-
4-hydroxy-2-oxoglutarate mutant Y140F, pH 8.5, 37°C Homo sapiens